##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062988_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	681124
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06993293438493	32.0	32.0	32.0	32.0	32.0
2	31.099501706003604	32.0	32.0	32.0	32.0	32.0
3	31.26629512394219	32.0	32.0	32.0	32.0	32.0
4	31.402436267111423	32.0	32.0	32.0	32.0	32.0
5	31.262542503274	32.0	32.0	32.0	32.0	32.0
6	34.80570204544253	36.0	36.0	36.0	32.0	36.0
7	34.85541692848879	36.0	36.0	36.0	32.0	36.0
8	34.762317874572034	36.0	36.0	36.0	32.0	36.0
9	34.926787192934036	36.0	36.0	36.0	32.0	36.0
10	34.72480928582755	36.0	36.0	36.0	32.0	36.0
11	34.941468807441815	36.0	36.0	36.0	36.0	36.0
12	34.82702709051509	36.0	36.0	36.0	32.0	36.0
13	34.894663820391	36.0	36.0	36.0	32.0	36.0
14	34.837747605428675	36.0	36.0	36.0	32.0	36.0
15	34.800062837310094	36.0	36.0	36.0	32.0	36.0
16	34.80512505799238	36.0	36.0	36.0	32.0	36.0
17	34.767215661171825	36.0	36.0	36.0	32.0	36.0
18	34.77469007111774	36.0	36.0	36.0	32.0	36.0
19	34.7779156218251	36.0	36.0	36.0	32.0	36.0
20	34.758927889782186	36.0	36.0	36.0	32.0	36.0
21	34.732464279631905	36.0	36.0	36.0	32.0	36.0
22	34.7208584633635	36.0	36.0	36.0	32.0	36.0
23	34.65311308954023	36.0	36.0	36.0	32.0	36.0
24	34.63076914042083	36.0	36.0	36.0	32.0	36.0
25	34.61102970971512	36.0	36.0	36.0	32.0	36.0
26	34.54019532419941	36.0	36.0	36.0	32.0	36.0
27	34.522045912344886	36.0	36.0	36.0	32.0	36.0
28	34.47893188318133	36.0	36.0	36.0	32.0	36.0
29	34.43057798579994	36.0	36.0	36.0	32.0	36.0
30	34.418082463692365	36.0	36.0	36.0	32.0	36.0
31	34.398917084113904	36.0	36.0	36.0	32.0	36.0
32	34.36921764612611	36.0	36.0	36.0	32.0	36.0
33	34.32748368872628	36.0	36.0	36.0	32.0	36.0
34	34.30722893335134	36.0	36.0	36.0	32.0	36.0
35	34.262585079956075	36.0	36.0	36.0	32.0	36.0
36	34.217848732389406	36.0	36.0	36.0	32.0	36.0
37	34.20707683182504	36.0	36.0	36.0	32.0	36.0
38	33.731919591733664	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	3.0
21	13.0
22	50.0
23	175.0
24	441.0
25	1082.0
26	2422.0
27	4819.0
28	8443.0
29	13661.0
30	20876.0
31	30585.0
32	45236.0
33	71816.0
34	166165.0
35	315337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.555205126059185	18.16438569366687	11.873833635064877	26.406575545209076
2	15.47148536830298	20.449874031747523	36.94231887292182	27.136321727027678
3	18.673545492450714	24.431674702403676	28.207345505370533	28.687434299775077
4	12.013689157464952	16.048202747521373	35.94798589975672	35.99012219525695
5	13.849627747117626	37.44257645094939	33.3378846405128	15.369911161420182
6	34.74541308985308	35.60795920854236	16.391753031390806	13.25487467021375
7	30.06263231921635	30.524373162946798	20.662205374105515	18.750789143731335
8	28.33085453282808	33.39861901692681	18.941314826261287	19.329211623983827
9	27.143805186301023	14.177171701154892	17.92095750709879	40.758065605445296
10	15.080588565203476	27.470144498571457	32.37842710618193	25.070839830043134
11	37.85549633675432	21.234051300121866	21.258864467251023	19.65158789587279
12	24.117888011087604	24.05284817235117	28.971651145684834	22.857612670876392
13	30.020275339402723	19.545662090400707	24.984474169863592	25.449588400332978
14	23.304420340496005	19.32540917659633	25.490512740705068	31.879657742202593
15	25.227124576435422	26.909784415172567	23.01563298312789	24.84745802526412
16	25.523840005637737	25.99820297038425	24.009871917595035	24.46808510638298
17	23.794786265055993	25.984108620456777	25.558929064311343	24.662176050175884
18	24.83116143316048	24.970489954839355	26.524685666633392	23.67366294536678
19	24.887538832870373	25.798973461513615	25.73569570298507	23.577792002630947
20	25.24063166178258	24.32112214516006	26.285522166301583	24.152724026755774
21	26.116830415607144	24.692273359916843	24.72780286702568	24.463093357450333
22	24.785574390490982	24.956028435434476	25.52097862057018	24.737418553504366
23	23.99408622831152	24.742997583399156	25.66177571712557	25.60114047116376
24	24.03057299405101	25.663755791896925	25.62661130719223	24.679059906859838
25	24.62048026497378	24.88930062661131	25.506956148953787	24.983262959461126
26	24.127765282092543	25.330776774860375	26.1389996535139	24.402458289533183
27	25.100275427088164	25.217141078570126	25.3061116624873	24.376471831854403
28	24.21233138165738	24.915287084290085	26.08981624491282	24.782565289139715
29	24.027783487294528	25.491100005285382	26.216665394260076	24.264451113160014
30	24.493337483336365	25.198935876580475	26.271868264809346	24.03585837527381
31	24.339914611730023	25.272931213699707	25.447201977907106	24.939952196663164
32	24.05993622306658	25.32314233531633	25.533383055067798	25.083538386549293
33	23.818717296703685	25.031418655046657	25.913343238529258	25.236520809720403
34	24.210275955626287	25.43193309881901	26.16836288252947	24.189428063025233
35	25.093815516704744	25.099100897927546	25.82657489678825	23.980508688579466
36	24.19324528279726	25.82995166812504	25.536025745679204	24.4407773033985
37	25.180143410010515	25.37056395017647	25.501964400021144	23.947328239791872
38	24.213359094672924	25.435309870155802	25.681814177741497	24.669516857429777
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	24.0
2	35.0
3	35.0
4	185.0
5	335.0
6	335.0
7	482.5
8	630.0
9	701.5
10	773.0
11	773.0
12	976.0
13	1179.0
14	1542.5
15	1906.0
16	1906.0
17	2732.5
18	3559.0
19	3559.0
20	4167.5
21	4776.0
22	5029.5
23	5283.0
24	5283.0
25	6159.0
26	7035.0
27	7035.0
28	9325.0
29	11615.0
30	13920.0
31	16225.0
32	16225.0
33	19602.0
34	22979.0
35	22979.0
36	25943.5
37	28908.0
38	33113.5
39	37319.0
40	37319.0
41	40434.5
42	43550.0
43	49107.0
44	54664.0
45	54664.0
46	60290.5
47	65917.0
48	65917.0
49	66775.0
50	67633.0
51	65222.5
52	62812.0
53	62812.0
54	60121.5
55	57431.0
56	57431.0
57	54775.0
58	52119.0
59	46848.0
60	41577.0
61	41577.0
62	37883.0
63	34189.0
64	28412.5
65	22636.0
66	22636.0
67	18733.0
68	14830.0
69	14830.0
70	11902.0
71	8974.0
72	6887.5
73	4801.0
74	4801.0
75	3674.0
76	2547.0
77	2547.0
78	2372.0
79	2197.0
80	1708.0
81	1219.0
82	1219.0
83	1067.5
84	916.0
85	916.0
86	603.0
87	290.0
88	236.5
89	183.0
90	183.0
91	110.5
92	38.0
93	27.5
94	17.0
95	17.0
96	12.0
97	7.0
98	7.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008074887979281306
2	0.0
3	0.0
4	1.468161450778419E-4
5	1.468161450778419E-4
6	1.468161450778419E-4
7	8.808968704670515E-4
8	0.0022022421761676288
9	0.003229955191712522
10	0.001468161450778419
11	0.004991748932646626
12	4.4044843523352573E-4
13	1.468161450778419E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	2.936322901556838E-4
23	2.936322901556838E-4
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	681124.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.499390641109656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1893904769151	50.919774311661094
2	13.091410354555547	16.625931602939808
3	3.5507634496464617	6.764139460898242
4	1.285598350613413	3.265388474926694
5	0.5574146624925054	1.7697745701346943
6	0.31622007169016747	1.2047869116488186
7	0.18535848437099728	0.8239105565402582
8	0.1315926792359155	0.668484395544931
9	0.09547037246337205	0.5456079428133509
>10	0.4714286952121173	5.755827364438264
>50	0.06940822359520638	3.108895017651961
>100	0.05246217635410795	6.358097091528929
>500	0.0023213352367443324	0.9040248866519379
>1k	0.0011606676183721662	1.2853574126210499
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2333	0.3425220664666052	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1933	0.2837956084354684	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1736	0.2548728278551336	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1674	0.2457702268603074	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1044	0.15327605546126696	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	867	0.12728959778248894	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	751	0.11025892495345928	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	685	0.10056905937832171	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.468161450778419E-4
2	0.0	0.0	0.0	0.0	1.468161450778419E-4
3	0.0	0.0	0.0	0.0	1.468161450778419E-4
4	0.0	0.0	0.0	0.0	1.468161450778419E-4
5	0.0	0.0	0.0	0.0	1.468161450778419E-4
6	0.0	0.0	0.0	0.0	2.936322901556838E-4
7	0.0	0.0	0.0	0.0	2.936322901556838E-4
8	0.0	0.0	0.0	0.0	2.936322901556838E-4
9	0.0	0.0	0.0	1.468161450778419E-4	2.936322901556838E-4
10	0.0	0.0	0.0	1.468161450778419E-4	2.936322901556838E-4
11	0.0	0.0	0.0	1.468161450778419E-4	2.936322901556838E-4
12	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	4.404484352335258E-4
13	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	5.872645803113676E-4
14	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	5.872645803113676E-4
15	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	5.872645803113676E-4
16	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	5.872645803113676E-4
17	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	5.872645803113676E-4
18	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	5.872645803113676E-4
19	1.468161450778419E-4	0.0	0.0	1.468161450778419E-4	5.872645803113676E-4
20	1.468161450778419E-4	0.0	0.0	4.404484352335258E-4	5.872645803113676E-4
21	1.468161450778419E-4	0.0	0.0	7.340807253892095E-4	5.872645803113676E-4
22	2.936322901556838E-4	0.0	0.0	8.808968704670516E-4	5.872645803113676E-4
23	2.936322901556838E-4	0.0	0.0	0.0016149775958562611	5.872645803113676E-4
24	5.872645803113676E-4	0.0	0.0	0.0027895067564789964	5.872645803113676E-4
25	5.872645803113676E-4	0.0	0.0	0.0032299551917125222	5.872645803113676E-4
26	5.872645803113676E-4	0.0	0.0	0.00381721977202389	5.872645803113676E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATCCG	30	8.41062E-4	26.668135	5
AAGACGG	170	0.0	22.589478	5
CGCAAGA	170	0.0	21.648249	2
GCGCAAG	185	0.0	19.892986	1
CAAGACG	190	0.0	19.369488	4
GACGGAC	200	0.0	19.201057	7
GTCCCGG	70	1.7846745E-5	18.28672	1
AGACGGA	220	0.0	18.18282	6
ATAGACG	55	0.0013633688	17.455507	3
CAACGGA	65	2.0991158E-4	17.230453	14
AAACGCT	65	2.0991158E-4	17.230453	29
AACGCTT	65	2.0991158E-4	17.230453	30
CGCGTAA	65	2.0991158E-4	17.230453	10
ACTGTTC	75	3.229041E-5	17.067606	8
ATCATAG	75	3.229041E-5	17.067606	1
ATCGAAC	75	3.229041E-5	17.067606	8
TAGCGGC	180	0.0	16.888578	30
CGTTTAT	125	2.8467184E-9	16.639696	31
AATAACG	135	4.4201443E-10	16.593506	2
TTAGGAC	70	3.6920886E-4	16.000881	3
>>END_MODULE