FastQCFastQC Report
Thu 2 Feb 2017
SRR4062987_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062987_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908091
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40860.44995490540044997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26310.2897286725669564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15580.1715687084223938No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA13470.14833315163348168No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11990.13203522554457647No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC11620.1279607440223502No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA11440.12597856382234818No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10110.111332454566778No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA9160.10087094795565642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC2050.017.94964430
TCTAGCG2250.017.06610928
GTATAGG957.6288416E-716.854551
CTAGCGG2200.016.72672729
CGTCGTA1456.91216E-1116.55209410
GATAACG1501.1459633E-1016.00035911
CGCGGTC1001.3133194E-616.00035710
CTAACAC805.6203447E-515.9994773
CCGATAA1451.2187229E-915.4486229
CGATAAC1451.2187229E-915.44862210
GTCCTAG1153.314599E-715.3156561
GAGTAGG1153.314599E-715.3156561
ATAATAG1052.1843425E-615.2375973
CCGGTCC2750.015.1276119
GTATTAC859.369522E-515.0699491
CGTTTAT1704.7293724E-1115.05750331
CGAACGA1501.9681465E-914.93366716
ACCGTCG1501.9717845E-914.9320238
CGGCATC2050.014.82878425
CGCGCTA650.00416158914.76874724
Produced by FastQC (version 0.11.5)