##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062987_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908091
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.132971255083465	32.0	32.0	32.0	32.0	32.0
2	30.709728430300487	32.0	32.0	32.0	32.0	32.0
3	30.778866875676556	32.0	32.0	32.0	32.0	32.0
4	30.83983983983984	32.0	32.0	32.0	32.0	32.0
5	30.728193540074727	32.0	32.0	32.0	32.0	32.0
6	34.387621945377724	36.0	36.0	36.0	32.0	36.0
7	34.296754400164744	36.0	36.0	36.0	32.0	36.0
8	34.26390526940582	36.0	36.0	36.0	32.0	36.0
9	34.41139709566552	36.0	36.0	36.0	32.0	36.0
10	34.107101601051	36.0	36.0	36.0	32.0	36.0
11	34.444038097503444	36.0	36.0	36.0	32.0	36.0
12	34.2416156530568	36.0	36.0	36.0	32.0	36.0
13	34.31757059589843	36.0	36.0	36.0	32.0	36.0
14	34.22459092756122	36.0	36.0	36.0	32.0	36.0
15	34.16620580977017	36.0	36.0	36.0	32.0	36.0
16	34.17551875307651	36.0	36.0	36.0	32.0	36.0
17	34.10413273559588	36.0	36.0	36.0	32.0	36.0
18	34.13239091676935	36.0	36.0	36.0	32.0	36.0
19	34.12257361872324	36.0	36.0	36.0	32.0	36.0
20	34.07758914029541	36.0	36.0	36.0	32.0	36.0
21	34.05922534195361	36.0	36.0	36.0	32.0	36.0
22	34.036114222032815	36.0	36.0	36.0	32.0	36.0
23	33.98345980744221	36.0	36.0	36.0	32.0	36.0
24	33.966905299138524	36.0	36.0	36.0	32.0	36.0
25	33.95444839779273	36.0	36.0	36.0	32.0	36.0
26	33.892026239661	36.0	36.0	36.0	32.0	36.0
27	33.92012804884092	36.0	36.0	36.0	32.0	36.0
28	33.883216549883215	36.0	36.0	36.0	32.0	36.0
29	33.848365417122295	36.0	36.0	36.0	32.0	36.0
30	33.81511874911215	36.0	36.0	36.0	32.0	36.0
31	33.831783378538056	36.0	36.0	36.0	32.0	36.0
32	33.77463932579444	36.0	36.0	36.0	32.0	36.0
33	33.728915934636504	36.0	36.0	36.0	27.0	36.0
34	33.72678839455517	36.0	36.0	36.0	27.0	36.0
35	33.67401284673012	36.0	36.0	36.0	27.0	36.0
36	33.61791714707006	36.0	36.0	36.0	27.0	36.0
37	33.61468399092161	36.0	36.0	36.0	27.0	36.0
38	32.9753130468202	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	0.0
8	3.0
9	2.0
10	6.0
11	6.0
12	5.0
13	5.0
14	229.0
15	352.0
16	437.0
17	493.0
18	604.0
19	774.0
20	1027.0
21	1484.0
22	2108.0
23	3210.0
24	4655.0
25	7015.0
26	9965.0
27	14092.0
28	19359.0
29	26441.0
30	35136.0
31	47636.0
32	63553.0
33	94050.0
34	199524.0
35	375918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65995880907964	17.5007387022655	11.524416866226533	25.314885622428324
2	16.06007018471692	20.649952347111352	38.36481729386683	24.9251601743049
3	18.819122761804696	25.49885130387473	28.843175996105668	26.838849938214903
4	12.25965456331464	16.19724514023179	36.66498544161891	34.87811485483466
5	14.001585745906242	37.238379455780816	33.71494014910416	15.045094649208778
6	32.430997876857745	36.27368713164363	17.815784373320646	13.479530618177973
7	28.71749637426205	31.383528743264716	21.723153296310613	18.17582158616262
8	27.59024721145174	32.89082898262174	20.33115696809454	19.187766837831973
9	27.90970929060918	14.23759124931587	18.93646988345856	38.91622957661639
10	15.82590654860162	27.144425589178127	31.945617545455445	25.084050316764806
11	36.37962419486453	21.75421247317133	22.883521992077764	18.982641339886374
12	24.69272709452696	24.387133576853742	29.395012934039155	21.525126394580145
13	29.690322211159764	20.12861573433659	25.440540278597673	24.74052177590597
14	23.74979625819923	20.22836702598644	26.016396259080278	30.00544045673405
15	24.87225720608143	27.26684943698444	23.548071934696143	24.312821422237985
16	24.891519823788546	26.31079295154185	24.711894273127754	24.08579295154185
17	23.432738006729778	25.98840187023973	25.984436697672113	24.59442342535838
18	24.194232236877607	25.124360302935862	27.4257830266495	23.25562443353703
19	25.029459379095403	25.380989614880566	26.262348160303073	23.327202845720958
20	25.352844783535673	24.394415273201417	26.362813419554914	23.889926523707995
21	26.761545221675743	24.448205912087378	25.01260960444387	23.77763926179301
22	25.336908819593955	24.82712670767096	25.700441062293855	24.13552341044123
23	23.954969758108923	24.662008872129444	26.05009625423453	25.332925115527104
24	24.067667806578445	25.802646577786586	26.074793631913124	24.05489198372184
25	24.51638199544467	24.874992840999706	26.15491195531022	24.453713208245407
26	23.825127102589633	25.86372487686248	26.65376549682354	23.657382523724348
27	24.63679534111283	25.407035906381743	25.86385001343571	24.09231873906972
28	23.79641865997093	25.398000088101846	26.433967666622614	24.371613585304612
29	23.7037852839789	25.552924591680526	26.475181444729568	24.268108679611007
30	23.833294790049226	25.757155285385487	26.580109462926867	23.82944046163842
31	24.24939897955925	25.627974128864512	25.538440529536725	24.584186362039514
32	23.75792643039168	26.00783227133666	25.53692686869933	24.69731442957233
33	23.704874341974847	25.340300874430078	26.134226118367437	24.82059866522764
34	24.322380629849285	25.640009030886734	26.272942031619117	23.764668307644865
35	24.913266531123824	25.413071587327114	26.221152172907438	23.452509708641628
36	23.869469876943782	26.246229179951957	25.667899101170867	24.21640184193339
37	24.685688357914398	26.085553558527604	25.556598121619	23.672159961938995
38	23.87169315956751	26.069829369126097	25.88459564645688	24.17388182484951
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	928.0
1	706.0
2	484.0
3	484.0
4	908.0
5	1332.0
6	1332.0
7	1524.0
8	1716.0
9	1602.5
10	1489.0
11	1489.0
12	1947.5
13	2406.0
14	2827.0
15	3248.0
16	3248.0
17	4722.5
18	6197.0
19	6197.0
20	7370.0
21	8543.0
22	8624.0
23	8705.0
24	8705.0
25	10213.0
26	11721.0
27	11721.0
28	14763.5
29	17806.0
30	21656.5
31	25507.0
32	25507.0
33	30511.5
34	35516.0
35	35516.0
36	38412.0
37	41308.0
38	46692.5
39	52077.0
40	52077.0
41	55302.5
42	58528.0
43	64874.5
44	71221.0
45	71221.0
46	74726.0
47	78231.0
48	78231.0
49	81070.5
50	83910.0
51	82341.0
52	80772.0
53	80772.0
54	76411.5
55	72051.0
56	72051.0
57	69452.5
58	66854.0
59	59895.5
60	52937.0
61	52937.0
62	49535.0
63	46133.0
64	37750.0
65	29367.0
66	29367.0
67	24270.5
68	19174.0
69	19174.0
70	15345.5
71	11517.0
72	9014.0
73	6511.0
74	6511.0
75	4889.0
76	3267.0
77	3267.0
78	3119.5
79	2972.0
80	2351.0
81	1730.0
82	1730.0
83	1782.0
84	1834.0
85	1834.0
86	1239.0
87	644.0
88	566.5
89	489.0
90	489.0
91	356.5
92	224.0
93	199.0
94	174.0
95	174.0
96	150.0
97	126.0
98	126.0
99	284.0
100	442.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12058262883345391
2	0.05351886540005352
3	0.011562717833344895
4	0.0025327858111136438
5	1.1012112222233234E-4
6	3.30363366666997E-4
7	0.0
8	2.2024224444466467E-4
9	2.2024224444466467E-4
10	4.4048448888932935E-4
11	0.001541695711112653
12	0.0024226646888913113
13	0.01266392905556822
14	0.008699568655564255
15	0.023345677911134455
16	0.010021022122232243
17	0.020482528733353815
18	0.004845329377782623
19	0.006717388455562273
20	0.004735208255560291
21	0.005836419477783614
22	0.006166782844450612
23	0.008699568655564255
24	0.013875261400013876
25	0.015747320477793522
26	0.01794974292224017
27	0.006937630700006938
28	0.00561617723333895
29	0.011122233344455567
30	0.0023125435666689793
31	0.005726298355561282
32	0.005836419477783614
33	0.007818599677785596
34	0.010571627733343905
35	0.015086593744459531
36	0.01453598813334787
37	0.008259084166674925
38	0.005395934988894285
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	908091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.73895317247754
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.13138468351364	51.324446661386645
2	11.076064892332461	13.676493034460727
3	2.909387251351643	5.388675698154066
4	1.0321977446084898	2.549072328764819
5	0.48402067272333105	1.4941464823888404
6	0.28441282919733757	1.0535610206079764
7	0.18584701795187217	0.803180023700266
8	0.12251316364484466	0.6051067578624914
9	0.0941956689804259	0.5233987796609438
>10	0.5275414869517996	6.245812500072715
>50	0.07021938631172658	3.066273231058702
>100	0.07451312472198	9.103962160693294
>500	0.006269133020210678	2.602411664185477
>1k	0.0014329446903338693	1.5634596570031138
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4086	0.44995490540044997	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2631	0.2897286725669564	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1558	0.1715687084223938	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1347	0.14833315163348168	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1199	0.13203522554457647	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1162	0.1279607440223502	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1144	0.12597856382234818	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1011	0.111332454566778	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	916	0.10087094795565642	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	3.30363366666997E-4	0.0
16	0.0	0.0	0.0	3.30363366666997E-4	0.0
17	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
18	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
19	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
20	0.0	0.0	0.0	5.506056111116617E-4	1.1012112222233234E-4
21	0.0	0.0	0.0	9.91090100000991E-4	1.1012112222233234E-4
22	0.0	0.0	0.0	0.0020923013222243145	1.1012112222233234E-4
23	0.0	0.0	0.0	0.0037441181555592997	1.1012112222233234E-4
24	0.0	0.0	0.0	0.00517569274444962	1.1012112222233234E-4
25	0.0	0.0	0.0	0.005946540600005946	1.1012112222233234E-4
26	0.0	0.0	0.0	0.007488236311118599	1.1012112222233234E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGC	205	0.0	17.949644	30
TCTAGCG	225	0.0	17.066109	28
GTATAGG	95	7.6288416E-7	16.85455	1
CTAGCGG	220	0.0	16.726727	29
CGTCGTA	145	6.91216E-11	16.552094	10
GATAACG	150	1.1459633E-10	16.000359	11
CGCGGTC	100	1.3133194E-6	16.000357	10
CTAACAC	80	5.6203447E-5	15.999477	3
CCGATAA	145	1.2187229E-9	15.448622	9
CGATAAC	145	1.2187229E-9	15.448622	10
GTCCTAG	115	3.314599E-7	15.315656	1
GAGTAGG	115	3.314599E-7	15.315656	1
ATAATAG	105	2.1843425E-6	15.237597	3
CCGGTCC	275	0.0	15.127611	9
GTATTAC	85	9.369522E-5	15.069949	1
CGTTTAT	170	4.7293724E-11	15.057503	31
CGAACGA	150	1.9681465E-9	14.933667	16
ACCGTCG	150	1.9717845E-9	14.932023	8
CGGCATC	205	0.0	14.828784	25
CGCGCTA	65	0.004161589	14.768747	24
>>END_MODULE