FastQCFastQC Report
Thu 2 Feb 2017
SRR4062987_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062987_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences908091
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50160.552367549067219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35380.38960853042261184No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT21760.23962356195579518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15990.17608367443350942No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14280.15725296253349058No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC13530.14899387836681566No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG10390.11441584598900331No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9650.10626688294455072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACAC852.3935354E-718.8243393
ATTAGGC450.0088466917.7785433
AACCGCG1650.017.4552967
GTATATA1302.582965E-1017.2305621
GTATTAG2250.017.066461
TATAATG957.684557E-716.8428292
CGGTAAT1051.18841854E-716.7617021
TTAACAC600.002440674716.0006893
CGCGAGG1407.421477E-1015.99980728
CGCTTCG1551.891749E-1015.48368532
GTATTAT1357.90169E-915.4072231
TAGGACT1255.12955E-815.3606614
CGAGGCG1603.0377123E-1014.9998230
CGGTCCG1603.0377123E-1014.9998220
CCCCTAT756.243134E-414.9331541
GAACCGC2150.014.8843616
TGGAACG650.00415949414.7698675
CGTATGT650.00416101714.76905323
GTATTAC650.00416101714.7690531
TGCTAGT650.00416101714.76905327
Produced by FastQC (version 0.11.5)