##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062987_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	908091
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.06120421851995	32.0	32.0	32.0	32.0	32.0
2	31.216258062242662	32.0	32.0	32.0	32.0	32.0
3	31.322988555111767	32.0	32.0	32.0	32.0	32.0
4	31.43817745137877	32.0	32.0	32.0	32.0	32.0
5	31.337119297515336	32.0	32.0	32.0	32.0	32.0
6	34.8578798820823	36.0	36.0	36.0	32.0	36.0
7	34.90096146751812	36.0	36.0	36.0	36.0	36.0
8	34.835517585792616	36.0	36.0	36.0	32.0	36.0
9	34.954144463495396	36.0	36.0	36.0	32.0	36.0
10	34.782433698825336	36.0	36.0	36.0	32.0	36.0
11	34.98422294681921	36.0	36.0	36.0	36.0	36.0
12	34.87943388933488	36.0	36.0	36.0	32.0	36.0
13	34.93343728767271	36.0	36.0	36.0	36.0	36.0
14	34.87086095996987	36.0	36.0	36.0	32.0	36.0
15	34.84767275526352	36.0	36.0	36.0	32.0	36.0
16	34.84849536004651	36.0	36.0	36.0	32.0	36.0
17	34.821009127939824	36.0	36.0	36.0	32.0	36.0
18	34.80729133974459	36.0	36.0	36.0	32.0	36.0
19	34.80228853716203	36.0	36.0	36.0	32.0	36.0
20	34.78253170662411	36.0	36.0	36.0	32.0	36.0
21	34.77974564223189	36.0	36.0	36.0	32.0	36.0
22	34.754665556645755	36.0	36.0	36.0	32.0	36.0
23	34.700628020760035	36.0	36.0	36.0	32.0	36.0
24	34.67345453264045	36.0	36.0	36.0	32.0	36.0
25	34.64881493154321	36.0	36.0	36.0	32.0	36.0
26	34.58111907286825	36.0	36.0	36.0	32.0	36.0
27	34.57174336052224	36.0	36.0	36.0	32.0	36.0
28	34.53545184348265	36.0	36.0	36.0	32.0	36.0
29	34.493551857688274	36.0	36.0	36.0	32.0	36.0
30	34.4550810436399	36.0	36.0	36.0	32.0	36.0
31	34.44877771060389	36.0	36.0	36.0	32.0	36.0
32	34.4148053443983	36.0	36.0	36.0	32.0	36.0
33	34.35878232467891	36.0	36.0	36.0	32.0	36.0
34	34.34492578386968	36.0	36.0	36.0	32.0	36.0
35	34.302185573912745	36.0	36.0	36.0	32.0	36.0
36	34.26494811643327	36.0	36.0	36.0	32.0	36.0
37	34.242534063216134	36.0	36.0	36.0	32.0	36.0
38	33.80607450134403	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	10.0
21	25.0
22	61.0
23	259.0
24	615.0
25	1586.0
26	3462.0
27	6583.0
28	11240.0
29	18148.0
30	27319.0
31	39130.0
32	55974.0
33	88335.0
34	209908.0
35	445435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.89911894273128	18.838656387665196	12.304625550660793	25.95759911894273
2	15.098156462292875	21.746278731977302	37.57266617552646	25.58289863020336
3	17.812862367317813	26.269613948381824	29.15423674499582	26.763286939304542
4	11.855018456361059	17.06024085771423	36.654266987340435	34.43047369858428
5	13.49359644969111	37.8113402856545	33.42565164245835	15.269411622196039
6	32.53675296501448	36.784019205144865	17.29740444229096	13.381823387549693
7	28.465491818533206	31.508695733567965	21.715933129902464	18.309879317996362
8	27.469088218062964	33.08617856812485	20.20135055964972	19.243382654162463
9	27.643669553419613	14.061357449320305	18.791241949835694	39.50373104742439
10	15.459862325966563	27.41158060846593	32.07324848830563	25.05530857726187
11	36.43914365005947	21.60092066428792	22.81969957270605	19.140236112946564
12	24.490440914932346	24.449365645878324	29.538734639446133	21.521458799743197
13	29.75989111187462	20.01854445824634	25.580670595801287	24.640893834077755
14	23.61106981569028	20.206785443309094	26.03362438345937	30.14852035754126
15	24.839140570713727	27.271495918360607	23.609417998856944	24.279945512068725
16	24.741463135302517	26.19087734599286	24.784300251847004	24.283359266857616
17	23.24205393512324	25.987153269881542	26.062365996359393	24.70842679863582
18	24.101108809579657	24.865569640047088	27.7083464102166	23.32497514015666
19	24.96633046688052	25.297905165891965	26.492388978637603	23.24337538858991
20	25.32995041245866	24.22070034831311	26.53599694303765	23.913352296190578
21	26.66244535233292	24.38568864319616	25.2526731931857	23.699192811285226
22	25.211019600458545	24.76326711750254	25.92702713714815	24.098686144890767
23	23.81636181435761	24.333270931295356	26.45442632338204	25.395940930964993
24	24.07016477423518	25.656789903214545	26.31234094380409	23.960704378746183
25	24.407025287113296	24.713051885769158	26.412220801659746	24.4677020254578
26	23.798275723468244	25.51594498789218	26.95963290022696	23.726146388412612
27	24.65768811461419	25.110726910328275	26.140250415707694	24.091334559349846
28	23.740572255423743	25.13822953866958	26.824404162137938	24.29679404376874
29	23.794091120823794	25.015554608513906	26.8360770010935	24.3542772695688
30	23.756209454779313	25.470244722169916	26.981326761304757	23.792219061746014
31	24.17720250503529	25.02579587288058	26.09319991058165	24.703801711502482
32	23.863467427823863	25.47938477531437	25.962706380748184	24.694441416113584
33	23.69443150521258	24.952234963236066	26.55119365790433	24.802139873647025
34	24.167621967401946	25.25991337872526	26.707785893704482	23.86467876016831
35	25.02623635736947	25.002670437213894	26.51529417205985	23.45579903335679
36	24.087233548179643	25.650292757003427	26.145397322515034	24.117076372301895
37	24.76029384720254	25.587193353970033	26.018868153081577	23.633644645745854
38	24.06642065607962	25.42080033829209	26.264438255637373	24.248340749990916
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	430.0
1	330.5
2	231.0
3	231.0
4	737.0
5	1243.0
6	1243.0
7	1470.5
8	1698.0
9	1720.0
10	1742.0
11	1742.0
12	2124.5
13	2507.0
14	3032.0
15	3557.0
16	3557.0
17	5372.5
18	7188.0
19	7188.0
20	8176.0
21	9164.0
22	9100.5
23	9037.0
24	9037.0
25	10116.5
26	11196.0
27	11196.0
28	14501.5
29	17807.0
30	21551.0
31	25295.0
32	25295.0
33	30275.0
34	35255.0
35	35255.0
36	38402.5
37	41550.0
38	46809.5
39	52069.0
40	52069.0
41	55374.0
42	58679.0
43	64950.0
44	71221.0
45	71221.0
46	74835.5
47	78450.0
48	78450.0
49	81877.0
50	85304.0
51	82829.0
52	80354.0
53	80354.0
54	76390.5
55	72427.0
56	72427.0
57	69697.0
58	66967.0
59	59823.5
60	52680.0
61	52680.0
62	49396.5
63	46113.0
64	37642.0
65	29171.0
66	29171.0
67	24218.5
68	19266.0
69	19266.0
70	15364.5
71	11463.0
72	8826.0
73	6189.0
74	6189.0
75	4658.5
76	3128.0
77	3128.0
78	3012.0
79	2896.0
80	2224.0
81	1552.0
82	1552.0
83	1521.5
84	1491.0
85	1491.0
86	948.5
87	406.0
88	340.0
89	274.0
90	274.0
91	157.5
92	41.0
93	31.5
94	22.0
95	22.0
96	18.0
97	14.0
98	14.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010021022122232243
2	0.0
3	0.0
4	3.30363366666997E-4
5	1.1012112222233234E-4
6	1.1012112222233234E-4
7	2.2024224444466467E-4
8	0.0025327858111136438
9	0.0038542392777816317
10	4.4048448888932935E-4
11	0.00561617723333895
12	2.2024224444466467E-4
13	3.30363366666997E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.1012112222233234E-4
22	0.0
23	1.1012112222233234E-4
24	0.0
25	0.0
26	0.0
27	1.1012112222233234E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	908091.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.60822699191598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6679792733682	48.680834464980215
2	12.099099468212767	14.424117349979928
3	3.106916022153474	5.555932664800348
4	1.1185385069732026	2.6669638889142977
5	0.5406722773721291	1.6114257918917014
6	0.3145451102592022	1.1249685798916658
7	0.20293525510987082	0.8467627525876091
8	0.13252003204720847	0.6319427320996787
9	0.09811967097850982	0.5263865658053212
>10	0.5567485372060348	6.397145594483302
>50	0.07194862519376669	3.043410686273501
>100	0.08216928050004493	9.942315099029043
>500	0.006506617187953508	2.7582635527338177
>1k	0.0011154200893634583	1.233688808508521
>5k	1.8590334822724306E-4	0.5558414680210851
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5016	0.552367549067219	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3538	0.38960853042261184	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2176	0.23962356195579518	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1599	0.17608367443350942	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1428	0.15725296253349058	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1353	0.14899387836681566	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1039	0.11441584598900331	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	965	0.10626688294455072	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.1012112222233234E-4
13	0.0	0.0	0.0	0.0	1.1012112222233234E-4
14	0.0	0.0	0.0	0.0	1.1012112222233234E-4
15	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
16	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
17	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
18	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
19	0.0	0.0	0.0	3.30363366666997E-4	1.1012112222233234E-4
20	0.0	0.0	0.0	5.506056111116617E-4	1.1012112222233234E-4
21	0.0	0.0	0.0	8.809689777786587E-4	1.1012112222233234E-4
22	0.0	0.0	0.0	0.001982180200001982	1.1012112222233234E-4
23	0.0	0.0	1.1012112222233234E-4	0.0036339970333369672	1.1012112222233234E-4
24	0.0	0.0	1.1012112222233234E-4	0.0050655716222272875	1.1012112222233234E-4
25	0.0	0.0	1.1012112222233234E-4	0.005726298355561282	1.1012112222233234E-4
26	0.0	0.0	1.1012112222233234E-4	0.006937630700006938	1.1012112222233234E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTACAC	85	2.3935354E-7	18.824339	3
ATTAGGC	45	0.00884669	17.778543	3
AACCGCG	165	0.0	17.455296	7
GTATATA	130	2.582965E-10	17.230562	1
GTATTAG	225	0.0	17.06646	1
TATAATG	95	7.684557E-7	16.842829	2
CGGTAAT	105	1.18841854E-7	16.761702	1
TTAACAC	60	0.0024406747	16.000689	3
CGCGAGG	140	7.421477E-10	15.999807	28
CGCTTCG	155	1.891749E-10	15.483685	32
GTATTAT	135	7.90169E-9	15.407223	1
TAGGACT	125	5.12955E-8	15.360661	4
CGAGGCG	160	3.0377123E-10	14.99982	30
CGGTCCG	160	3.0377123E-10	14.99982	20
CCCCTAT	75	6.243134E-4	14.933154	1
GAACCGC	215	0.0	14.884361	6
TGGAACG	65	0.004159494	14.769867	5
CGTATGT	65	0.004161017	14.769053	23
GTATTAC	65	0.004161017	14.769053	1
TGCTAGT	65	0.004161017	14.769053	27
>>END_MODULE