FastQCFastQC Report
Thu 2 Feb 2017
SRR4062986_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062986_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225507
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26970.22007218237023535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT17560.1432876352399456No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14660.11962395971626437No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14570.11888956978621909No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT14210.11595201006603797No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14200.11587041118492182No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13030.10632334209433321No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13030.10632334209433321No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG12940.10558895216428793No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12740.10395697454196509No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG12700.1036305790175005No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12350.10077461817843553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT3550.018.9491731
CTATTCC3300.018.906274
CATCGTT3050.017.8348628
CGTTTAT2900.017.65397831
TCGTTTA3000.017.06551430
ATCGTTT3000.017.06551429
CCTATTC3900.016.8193763
CCCTTAT1051.1753036E-716.7792571
TACCGTC2450.016.3247627
GTATTTA1407.3123374E-1016.0165631
GTCTTAC805.5718112E-516.0165631
CGAGCCG4600.016.00022515
AGGCCCG4650.015.82817910
ACCGTCG2450.015.672418
TATTCCA4000.015.5976725
ATACCGT2700.015.4051096
GTGTAGG1052.161718E-615.253871
AACCGAC1052.1847954E-615.2383117
AAACCGA1052.1847954E-615.2383116
GTTTATA951.4185849E-515.1735871
Produced by FastQC (version 0.11.5)