##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062986_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225507
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.253503243963518	32.0	32.0	32.0	32.0	32.0
2	30.836018888509003	32.0	32.0	32.0	32.0	32.0
3	30.88700513338561	32.0	32.0	32.0	32.0	32.0
4	30.971001389628945	32.0	32.0	32.0	32.0	32.0
5	30.860140333755744	32.0	32.0	32.0	32.0	32.0
6	34.5241332770845	36.0	36.0	36.0	32.0	36.0
7	34.45101496768277	36.0	36.0	36.0	32.0	36.0
8	34.44066170164675	36.0	36.0	36.0	32.0	36.0
9	34.59387747275209	36.0	36.0	36.0	32.0	36.0
10	34.28706649574421	36.0	36.0	36.0	32.0	36.0
11	34.577749453899486	36.0	36.0	36.0	32.0	36.0
12	34.38157350386412	36.0	36.0	36.0	32.0	36.0
13	34.47602584073367	36.0	36.0	36.0	32.0	36.0
14	34.36729288367998	36.0	36.0	36.0	32.0	36.0
15	34.31180482853219	36.0	36.0	36.0	32.0	36.0
16	34.332996057958056	36.0	36.0	36.0	32.0	36.0
17	34.252462858229286	36.0	36.0	36.0	32.0	36.0
18	34.275863785355774	36.0	36.0	36.0	32.0	36.0
19	34.26603356814771	36.0	36.0	36.0	32.0	36.0
20	34.24939637227694	36.0	36.0	36.0	32.0	36.0
21	34.24033236856256	36.0	36.0	36.0	32.0	36.0
22	34.20322935731905	36.0	36.0	36.0	32.0	36.0
23	34.15146873906065	36.0	36.0	36.0	32.0	36.0
24	34.14422439039516	36.0	36.0	36.0	32.0	36.0
25	34.10930659718794	36.0	36.0	36.0	32.0	36.0
26	34.06441905268595	36.0	36.0	36.0	32.0	36.0
27	34.06660427072224	36.0	36.0	36.0	32.0	36.0
28	34.048256762303275	36.0	36.0	36.0	32.0	36.0
29	34.012396502019165	36.0	36.0	36.0	32.0	36.0
30	33.98566062862146	36.0	36.0	36.0	32.0	36.0
31	34.01459069593238	36.0	36.0	36.0	32.0	36.0
32	33.95646618093573	36.0	36.0	36.0	32.0	36.0
33	33.93473558290569	36.0	36.0	36.0	32.0	36.0
34	33.94156785722154	36.0	36.0	36.0	32.0	36.0
35	33.88510143148917	36.0	36.0	36.0	32.0	36.0
36	33.849546351020436	36.0	36.0	36.0	32.0	36.0
37	33.84920526769737	36.0	36.0	36.0	32.0	36.0
38	33.264701058419085	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	1.0
10	11.0
11	15.0
12	8.0
13	6.0
14	151.0
15	374.0
16	444.0
17	475.0
18	593.0
19	786.0
20	1057.0
21	1497.0
22	2327.0
23	3460.0
24	5326.0
25	8151.0
26	11761.0
27	17306.0
28	24342.0
29	32242.0
30	43397.0
31	58852.0
32	79936.0
33	120529.0
34	268693.0
35	543761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.88745189990278	16.763506834094493	11.174336810973767	26.174704455028962
2	17.13706270069292	19.456997319603957	36.69603427118619	26.709905708516935
3	19.061908337141737	23.395165502382973	28.312740745576807	29.23018541489848
4	12.942142259113766	14.992076405968726	35.43405138014156	36.63172995477594
5	15.046437961136053	36.03833844958736	33.26030227514186	15.654921314134732
6	34.66443193983863	34.90368044494347	16.661145128861268	13.770742486356632
7	30.710310100227904	30.154458522064747	20.613754144203174	18.521477233504175
8	28.656886192853058	32.13325467175519	19.360621720224266	19.849237415167487
9	27.776526593913044	14.080143222473001	18.295300063810377	39.848030119803575
10	16.235034728568817	25.96360354596966	31.161628195420004	26.639733530041514
11	37.88837716363494	20.826628499433696	21.93639411040862	19.348600226522745
12	25.187784328525677	23.36432811766866	28.149737856749425	23.298149697056246
13	29.96387063287193	18.79608493788148	24.843081679138248	26.396962750108337
14	24.14846962008582	19.313732370803553	24.53283955533866	32.00495845377196
15	25.706573904186065	26.406291829632195	22.04613420741621	25.841000058765534
16	26.4559034272489	25.23160616180114	23.029552228087187	25.282938182862775
17	24.651783076322882	25.342340533543577	24.729890095181197	25.275986294952347
18	25.731963373066097	24.10062696001508	25.59331895478919	24.57409071212963
19	26.197616640267402	24.421807178477785	24.970275327583437	24.410300853671373
20	26.274050898384395	23.69404172603397	24.494255856349895	25.53765151923174
21	27.445645743178126	23.706232817298048	23.940762163251684	24.907359276272143
22	26.417559424717812	23.72981909816748	24.341529733576188	25.511091743538522
23	24.803592660930793	23.446062346018035	25.129530507504978	26.6208144855462
24	25.29439272548618	24.52340982225853	24.651947778496865	25.530249673758426
25	25.483231752104807	23.7978375766756	24.77311530639227	25.945815364827325
26	25.1200758691779	24.740974824262793	25.10685426115288	25.03209504540643
27	25.88052880692019	24.206871225722214	24.221152276807572	25.691447690550024
28	25.10198435981404	23.946637637471817	25.25409385906859	25.697284143645554
29	25.01585229011527	24.492257472202386	25.074120167295728	25.417770070386613
30	25.060976953200548	24.52550587002627	25.26628710944353	25.147230067329655
31	25.680490272881578	24.39270792531662	23.971553074813944	25.955248726987858
32	25.211581037312197	24.46726298068331	24.02684147518763	26.294314506816864
33	24.875632040581166	23.958295930458398	24.821853506947956	26.344218522012476
34	25.73005731428352	24.091422117290666	24.78657100956544	25.39194955886037
35	26.366978047717527	24.025988864713284	24.777155523490688	24.829877564078497
36	25.225840623319307	24.690221502949047	24.333740576882114	25.750197296849535
37	26.102717356709988	24.515294006277227	24.290302942266166	25.09168569474662
38	25.185852522453267	24.30624578720682	24.864252175147744	25.64364951519217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	374.0
1	318.0
2	262.0
3	262.0
4	497.0
5	732.0
6	732.0
7	875.0
8	1018.0
9	964.0
10	910.0
11	910.0
12	1164.5
13	1419.0
14	1824.0
15	2229.0
16	2229.0
17	3278.0
18	4327.0
19	4327.0
20	5368.5
21	6410.0
22	6751.0
23	7092.0
24	7092.0
25	8289.0
26	9486.0
27	9486.0
28	12277.0
29	15068.0
30	18715.5
31	22363.0
32	22363.0
33	29018.5
34	35674.0
35	35674.0
36	40849.0
37	46024.0
38	53070.5
39	60117.0
40	60117.0
41	65566.5
42	71016.0
43	81563.0
44	92110.0
45	92110.0
46	97348.5
47	102587.0
48	102587.0
49	109601.0
50	116615.0
51	118301.5
52	119988.0
53	119988.0
54	115461.0
55	110934.0
56	110934.0
57	107889.0
58	104844.0
59	94876.0
60	84908.0
61	84908.0
62	79625.5
63	74343.0
64	61705.0
65	49067.0
66	49067.0
67	41121.5
68	33176.0
69	33176.0
70	26741.0
71	20306.0
72	15920.5
73	11535.0
74	11535.0
75	8768.0
76	6001.0
77	6001.0
78	5715.5
79	5430.0
80	4196.0
81	2962.0
82	2962.0
83	3011.0
84	3060.0
85	3060.0
86	2034.0
87	1008.0
88	869.5
89	731.0
90	731.0
91	540.5
92	350.0
93	287.0
94	224.0
95	224.0
96	207.0
97	190.0
98	190.0
99	403.5
100	617.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12215352503086477
2	0.05614003020790579
3	0.011995035524072894
4	0.0043247406991555335
5	8.159888111614214E-5
6	2.4479664334842643E-4
7	0.0
8	3.2639552446456856E-4
9	8.159888111614214E-5
10	2.4479664334842643E-4
11	0.0018767742656712692
12	0.0026111641957165485
13	0.015340589649834722
14	0.010199860139517767
15	0.0243980654537265
16	0.012321431048537463
17	0.021623703495777663
18	0.00571192167812995
19	0.007670294824917361
20	0.006935904894872082
21	0.006038317202594518
22	0.007425498181568934
23	0.009791865733937057
24	0.015340589649834722
25	0.01754375943997056
26	0.019583731467874115
27	0.00873108027942721
28	0.006038317202594518
29	0.010771052307330761
30	0.003590350769110254
31	0.005467125034781523
32	0.007343899300452793
33	0.009057475803891777
34	0.012892623216350459
35	0.017380561677738274
36	0.01599338069876386
37	0.010199860139517767
38	0.005956718321478376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1225507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.525647320581875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.809408910719	46.243297955925705
2	10.788135749185507	12.196127132100424
3	2.9928353042662708	5.075158586926246
4	1.3713487399071784	3.100655009020701
5	0.752154867740278	2.1258020392172927
6	0.4843407079868565	1.642660322559959
7	0.32411698001393197	1.282464547201572
8	0.230906188866391	1.044169743680103
9	0.17072054514426296	0.8685080392681874
>10	0.9168507282928615	9.224383308411877
>50	0.07207970170429649	2.885445863321416
>100	0.07536243421857505	8.914187812924826
>500	0.009130443742443525	3.415545110762351
>1k	0.002608698212126721	1.981594528679407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2697	0.22007218237023535	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1756	0.1432876352399456	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1466	0.11962395971626437	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1457	0.11888956978621909	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1421	0.11595201006603797	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1420	0.11587041118492182	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1303	0.10632334209433321	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1303	0.10632334209433321	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1294	0.10558895216428793	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1274	0.10395697454196509	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1270	0.1036305790175005	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1235	0.10077461817843553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4479664334842643E-4
2	0.0	0.0	0.0	0.0	2.4479664334842643E-4
3	0.0	0.0	0.0	0.0	2.4479664334842643E-4
4	0.0	0.0	0.0	0.0	2.4479664334842643E-4
5	0.0	0.0	0.0	0.0	3.2639552446456856E-4
6	0.0	0.0	0.0	0.0	4.079944055807107E-4
7	0.0	0.0	0.0	8.159888111614214E-5	4.079944055807107E-4
8	0.0	0.0	0.0	1.6319776223228428E-4	4.895932866968529E-4
9	0.0	8.159888111614214E-5	0.0	2.4479664334842643E-4	4.895932866968529E-4
10	0.0	8.159888111614214E-5	0.0	2.4479664334842643E-4	4.895932866968529E-4
11	0.0	8.159888111614214E-5	0.0	2.4479664334842643E-4	4.895932866968529E-4
12	0.0	8.159888111614214E-5	0.0	2.4479664334842643E-4	4.895932866968529E-4
13	0.0	8.159888111614214E-5	0.0	2.4479664334842643E-4	6.527910489291371E-4
14	0.0	8.159888111614214E-5	0.0	2.4479664334842643E-4	6.527910489291371E-4
15	8.159888111614214E-5	8.159888111614214E-5	0.0	2.4479664334842643E-4	8.159888111614214E-4
16	8.159888111614214E-5	8.159888111614214E-5	0.0	2.4479664334842643E-4	8.975876922775634E-4
17	8.159888111614214E-5	8.159888111614214E-5	0.0	2.4479664334842643E-4	8.975876922775634E-4
18	8.159888111614214E-5	8.159888111614214E-5	0.0	2.4479664334842643E-4	8.975876922775634E-4
19	8.159888111614214E-5	1.6319776223228428E-4	0.0	2.4479664334842643E-4	9.791865733937057E-4
20	8.159888111614214E-5	1.6319776223228428E-4	0.0	4.079944055807107E-4	9.791865733937057E-4
21	8.159888111614214E-5	1.6319776223228428E-4	0.0	7.343899300452792E-4	9.791865733937057E-4
22	8.159888111614214E-5	1.6319776223228428E-4	0.0	0.0011423843356259899	0.0010607854545098478
23	8.159888111614214E-5	1.6319776223228428E-4	0.0	0.0017135765034389848	0.0011423843356259899
24	8.159888111614214E-5	1.6319776223228428E-4	0.0	0.0026111641957165485	0.0011423843356259899
25	8.159888111614214E-5	1.6319776223228428E-4	0.0	0.0031007574824134013	0.001223983216742132
26	8.159888111614214E-5	1.6319776223228428E-4	0.0	0.003998345174690964	0.001223983216742132
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAT	355	0.0	18.949173	1
CTATTCC	330	0.0	18.90627	4
CATCGTT	305	0.0	17.83486	28
CGTTTAT	290	0.0	17.653978	31
TCGTTTA	300	0.0	17.065514	30
ATCGTTT	300	0.0	17.065514	29
CCTATTC	390	0.0	16.819376	3
CCCTTAT	105	1.1753036E-7	16.779257	1
TACCGTC	245	0.0	16.324762	7
GTATTTA	140	7.3123374E-10	16.016563	1
GTCTTAC	80	5.5718112E-5	16.016563	1
CGAGCCG	460	0.0	16.000225	15
AGGCCCG	465	0.0	15.828179	10
ACCGTCG	245	0.0	15.67241	8
TATTCCA	400	0.0	15.597672	5
ATACCGT	270	0.0	15.405109	6
GTGTAGG	105	2.161718E-6	15.25387	1
AACCGAC	105	2.1847954E-6	15.23831	17
AAACCGA	105	2.1847954E-6	15.23831	16
GTTTATA	95	1.4185849E-5	15.173587	1
>>END_MODULE