FastQCFastQC Report
Thu 2 Feb 2017
SRR4062986_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062986_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1225507
Sequences flagged as poor quality0
Sequence length38
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32330.2638091826484875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22610.18449507020359737No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15100.12321431048537464No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG14610.11921596531068365No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14130.11529921901710884No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13560.11064808279348873No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC13450.10975049510121117No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13410.1094240995767466No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13410.1094240995767466No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13360.10901610517116589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13310.10860811076558519No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12780.10428337006642965No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCA350.00206667922.8570254
CTAGCGG3050.022.03177629
TCTAGCG3200.021.49901428
TAGCGGC3350.019.5811930
GTAATAC1105.2023097E-1018.9089953
GAGTAAT601.1383051E-418.666571
CATCGTT3450.016.69488528
AGCGGCG4150.016.5775531
CGCGTAA1203.1059244E-816.00187710
GCGTAAC1203.1059244E-816.00187711
CGTTTAT3500.015.99926431
ATCGTTT3650.015.78009829
TCGTTTA3600.015.55484230
ACGAACG1652.910383E-1115.5144415
ATACTGC1255.1273673E-815.362436
CCGATAA1704.7293724E-1115.0612059
CGAACGA1704.7293724E-1115.05813216
CTAGATA3200.014.9999233
ATACCGT2350.014.9810926
ACCGTCG2250.014.9356958
Produced by FastQC (version 0.11.5)