##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1225507
Sequences flagged as poor quality	0
Sequence length	38
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.20226730651069	32.0	32.0	32.0	32.0	32.0
2	31.316358046098472	32.0	32.0	32.0	32.0	32.0
3	31.39201816064698	32.0	32.0	32.0	32.0	32.0
4	31.486094326674593	32.0	32.0	32.0	32.0	32.0
5	31.414421133457417	32.0	32.0	32.0	32.0	32.0
6	34.94095586561317	36.0	36.0	36.0	36.0	36.0
7	34.96882188351433	36.0	36.0	36.0	36.0	36.0
8	34.91184464878618	36.0	36.0	36.0	36.0	36.0
9	35.004606256839004	36.0	36.0	36.0	36.0	36.0
10	34.85752427362716	36.0	36.0	36.0	32.0	36.0
11	35.03725804911763	36.0	36.0	36.0	36.0	36.0
12	34.93053732047226	36.0	36.0	36.0	32.0	36.0
13	34.987231407082945	36.0	36.0	36.0	36.0	36.0
14	34.92702612061783	36.0	36.0	36.0	32.0	36.0
15	34.898540767209	36.0	36.0	36.0	32.0	36.0
16	34.909004191734525	36.0	36.0	36.0	32.0	36.0
17	34.8782095899901	36.0	36.0	36.0	32.0	36.0
18	34.87547684346152	36.0	36.0	36.0	32.0	36.0
19	34.873130875629435	36.0	36.0	36.0	32.0	36.0
20	34.858225208015945	36.0	36.0	36.0	32.0	36.0
21	34.84936030557149	36.0	36.0	36.0	32.0	36.0
22	34.830268615356744	36.0	36.0	36.0	32.0	36.0
23	34.77181852082444	36.0	36.0	36.0	32.0	36.0
24	34.750031619566435	36.0	36.0	36.0	32.0	36.0
25	34.73568490428859	36.0	36.0	36.0	32.0	36.0
26	34.66969588912997	36.0	36.0	36.0	32.0	36.0
27	34.65011460562853	36.0	36.0	36.0	32.0	36.0
28	34.61901319209111	36.0	36.0	36.0	32.0	36.0
29	34.588896677048766	36.0	36.0	36.0	32.0	36.0
30	34.5625581902021	36.0	36.0	36.0	32.0	36.0
31	34.56907059690398	36.0	36.0	36.0	32.0	36.0
32	34.5329426922898	36.0	36.0	36.0	32.0	36.0
33	34.4992252186238	36.0	36.0	36.0	32.0	36.0
34	34.49252431850655	36.0	36.0	36.0	32.0	36.0
35	34.4586501749888	36.0	36.0	36.0	32.0	36.0
36	34.43724678847204	36.0	36.0	36.0	32.0	36.0
37	34.4232950117788	36.0	36.0	36.0	32.0	36.0
38	33.99971277193847	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	1.0
20	3.0
21	27.0
22	90.0
23	301.0
24	831.0
25	2080.0
26	4179.0
27	8278.0
28	14395.0
29	22360.0
30	33984.0
31	48399.0
32	68242.0
33	109702.0
34	270748.0
35	641885.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.199120608653715	17.959136375024176	11.886880372256083	26.954862644066026
2	16.184743281550638	20.4410259925288	36.07448678341844	27.29974394250212
3	17.954773004152567	24.068161177373938	28.693593753442453	29.283472065031045
4	12.420828620994104	15.913916374134441	35.370859057401596	36.29439594746986
5	14.48014126409826	36.56530445383376	32.97568514556436	15.978869136503615
6	34.76204930861211	35.25572294219694	16.32901022108419	13.653217528106756
7	30.354818730982235	30.361591482326787	20.602872788961886	18.68071699772909
8	28.49048089610382	32.3545899861524	19.26244590908238	19.892483208661403
9	27.777428702225045	13.830736075217532	18.165438419400402	40.22639680315702
10	16.004674041119813	26.2867485059062	31.173846912097346	26.534730540876645
11	37.9596489767807	20.797978858309545	21.85791948400827	19.384452680901486
12	25.052917263022866	23.38037271378656	28.164795046585144	23.40191497660543
13	29.88417019922399	18.76916046854154	24.971093549189927	26.375575783044543
14	23.972853684230284	19.16627159208393	24.63347822574657	32.22739649793922
15	25.65917616137647	26.472472209461063	21.96078847366845	25.907563155494014
16	26.45378606568547	25.251100156914646	23.016351599786862	25.278762177613018
17	24.641066921690367	25.38435112977731	24.72609295581339	25.248488992718933
18	25.56949899102984	24.07109873709412	25.709196275500673	24.650205996375377
19	26.088712671571844	24.499656060716095	25.00850668335636	24.403124584355698
20	26.210131806672663	23.507250468581574	24.73670081035849	25.545916914387266
21	27.318533992109373	23.734134091660174	23.995250937368677	24.952080978861776
22	26.248075286391675	23.74600879472741	24.560610424909854	25.445305493971066
23	24.76774880559443	23.372732057396746	25.1502033855431	26.709315751465724
24	25.242287477754104	24.48945620057658	24.73531362937951	25.5329426922898
25	25.499223176750135	23.750799670992507	24.90632425516359	25.843652897093765
26	25.265400577394153	24.492903339518534	25.131823100009303	25.109872983078006
27	25.93012676406869	24.100840061852054	24.326950930432762	25.642082243646495
28	25.118746771744267	23.86889670968832	25.34330689257589	25.66904962599153
29	25.094675101815007	24.36101956169977	25.172765231043154	25.371540105442076
30	25.098510249227463	24.347800542958957	25.401568493692817	25.15212071412077
31	25.582554812008418	24.176279694852823	24.238539641144442	26.002625851994317
32	25.232985205306864	24.31385540841464	24.23715246016547	26.216006926113028
33	24.868890997766638	23.7500071399021	25.021888899859405	26.359212962471858
34	25.647181125852402	23.907574579337368	25.13824890433102	25.3069953904792
35	26.394382080232916	23.793172948012536	24.89989857259077	24.912546399163773
36	25.205731179014073	24.525686103792143	24.549594575959173	25.718988141234604
37	26.15023822793342	24.36501790687446	24.465955722815128	25.01878814237699
38	25.29210407138282	24.042415167624775	24.974683905818416	25.690796855173993
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	170.0
1	143.5
2	117.0
3	117.0
4	455.5
5	794.0
6	794.0
7	889.5
8	985.0
9	952.0
10	919.0
11	919.0
12	1153.0
13	1387.0
14	1823.5
15	2260.0
16	2260.0
17	3520.5
18	4781.0
19	4781.0
20	5716.5
21	6652.0
22	6955.5
23	7259.0
24	7259.0
25	8316.0
26	9373.0
27	9373.0
28	12128.0
29	14883.0
30	18498.0
31	22113.0
32	22113.0
33	29275.0
34	36437.0
35	36437.0
36	41169.5
37	45902.0
38	53104.5
39	60307.0
40	60307.0
41	65625.5
42	70944.0
43	82145.0
44	93346.0
45	93346.0
46	98286.0
47	103226.0
48	103226.0
49	110495.0
50	117764.0
51	118811.5
52	119859.0
53	119859.0
54	115381.0
55	110903.0
56	110903.0
57	107994.0
58	105085.0
59	95144.5
60	85204.0
61	85204.0
62	79962.0
63	74720.0
64	61690.5
65	48661.0
66	48661.0
67	40768.0
68	32875.0
69	32875.0
70	26379.5
71	19884.0
72	15601.5
73	11319.0
74	11319.0
75	8483.5
76	5648.0
77	5648.0
78	5400.5
79	5153.0
80	3950.0
81	2747.0
82	2747.0
83	2663.5
84	2580.0
85	2580.0
86	1615.5
87	651.0
88	561.0
89	471.0
90	471.0
91	275.5
92	80.0
93	52.0
94	24.0
95	24.0
96	16.0
97	8.0
98	8.0
99	12.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009302272447240204
2	8.159888111614214E-5
3	0.0
4	8.159888111614214E-5
5	8.159888111614214E-5
6	8.159888111614214E-5
7	6.527910489291371E-4
8	0.0019583731467874115
9	0.003916746293574823
10	0.0012239832167421322
11	0.00497753174808467
12	7.343899300452792E-4
13	1.6319776223228428E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.6319776223228428E-4
22	0.0
23	1.6319776223228428E-4
24	0.0
25	2.4479664334842643E-4
26	8.159888111614214E-5
27	1.6319776223228428E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.6319776223228428E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1225507.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.69903643780242
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.6131011675963	44.094589581306025
2	11.511229871304387	12.593063643487968
3	3.2155661757657374	5.276651142491252
4	1.4302122590925035	3.1292492989557026
5	0.8070162666339469	2.2071506087254766
6	0.49380999866237174	1.6206558666110542
7	0.34833840481844314	1.3337642568494994
8	0.24566477237322332	1.0750101068421902
9	0.18483608270453555	0.9099320060588447
>10	0.9791450699435721	9.48019238119604
>50	0.07671235519419689	2.9610527950520495
>100	0.08161520652425384	9.40976630691265
>500	0.010051867634116722	3.786752083527441
>1k	0.0027005017524492684	2.1221699219837817
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3233	0.2638091826484875	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2261	0.18449507020359737	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1510	0.12321431048537464	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1461	0.11921596531068365	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1413	0.11529921901710884	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1356	0.11064808279348873	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1345	0.10975049510121117	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1341	0.1094240995767466	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1341	0.1094240995767466	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1336	0.10901610517116589	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1331	0.10860811076558519	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1278	0.10428337006642965	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.159888111614214E-5
2	0.0	0.0	0.0	0.0	8.159888111614214E-5
3	0.0	0.0	0.0	0.0	8.159888111614214E-5
4	0.0	0.0	0.0	0.0	8.159888111614214E-5
5	0.0	0.0	0.0	0.0	1.6319776223228428E-4
6	8.159888111614214E-5	0.0	0.0	0.0	1.6319776223228428E-4
7	8.159888111614214E-5	0.0	0.0	0.0	1.6319776223228428E-4
8	8.159888111614214E-5	0.0	0.0	0.0	1.6319776223228428E-4
9	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	1.6319776223228428E-4
10	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	1.6319776223228428E-4
11	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	1.6319776223228428E-4
12	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	4.895932866968529E-4
13	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	5.711921678129949E-4
14	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	5.711921678129949E-4
15	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	7.343899300452792E-4
16	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	7.343899300452792E-4
17	8.159888111614214E-5	0.0	0.0	8.159888111614214E-5	7.343899300452792E-4
18	8.159888111614214E-5	0.0	0.0	1.6319776223228428E-4	7.343899300452792E-4
19	8.159888111614214E-5	0.0	0.0	2.4479664334842643E-4	7.343899300452792E-4
20	8.159888111614214E-5	0.0	0.0	3.2639552446456856E-4	8.975876922775634E-4
21	8.159888111614214E-5	0.0	0.0	6.527910489291371E-4	0.0011423843356259899
22	8.159888111614214E-5	0.0	0.0	0.0010607854545098478	0.0011423843356259899
23	8.159888111614214E-5	0.0	0.0	0.0016319776223228427	0.001223983216742132
24	8.159888111614214E-5	0.0	0.0	0.002529565314600406	0.001223983216742132
25	8.159888111614214E-5	0.0	0.0	0.003019158601297259	0.0013055820978582742
26	8.159888111614214E-5	0.0	0.0	0.00383514741245868	0.0013871809789744163
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACGCA	35	0.002066679	22.857025	4
CTAGCGG	305	0.0	22.031776	29
TCTAGCG	320	0.0	21.499014	28
TAGCGGC	335	0.0	19.58119	30
GTAATAC	110	5.2023097E-10	18.908995	3
GAGTAAT	60	1.1383051E-4	18.66657	1
CATCGTT	345	0.0	16.694885	28
AGCGGCG	415	0.0	16.57755	31
CGCGTAA	120	3.1059244E-8	16.001877	10
GCGTAAC	120	3.1059244E-8	16.001877	11
CGTTTAT	350	0.0	15.999264	31
ATCGTTT	365	0.0	15.780098	29
TCGTTTA	360	0.0	15.554842	30
ACGAACG	165	2.910383E-11	15.51444	15
ATACTGC	125	5.1273673E-8	15.36243	6
CCGATAA	170	4.7293724E-11	15.061205	9
CGAACGA	170	4.7293724E-11	15.058132	16
CTAGATA	320	0.0	14.999923	3
ATACCGT	235	0.0	14.981092	6
ACCGTCG	225	0.0	14.935695	8
>>END_MODULE