FastQCFastQC Report
Thu 2 Feb 2017
SRR4062985_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062985_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146429
Sequences flagged as poor quality0
Sequence length38
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39802.718040825246365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27171.8555067643704455No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA23201.5843856066762732No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC20001.3658496609278217No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA17951.22585007068272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14911.018240922221691No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12170.8311195186745794No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA9560.6528761379234987No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG9320.636485941992365No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA8100.5531691126757678No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA7470.5101448483565414No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG6690.45687671158035636No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA6050.413169522430666No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5130.35034043802798626No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA5110.34897458836705847No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4590.31346249718293506No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.29638937642133734No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC4100.27999918049020345No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3910.26702360871138914No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA3530.24107246515376052No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA3490.23834076583190486No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC3450.23560906651004923No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA3450.23560906651004923No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3170.2164871712570597No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA3130.21375547193520408No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.20419452430870932No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA2960.20214574981731762No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT2850.1946335766822146No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2840.19395065185175067No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC2700.1843897042252559No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2630.17960923041200855No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA2410.16458488414180253No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2250.15365808685437995No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2110.1440971392278852No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC2060.14068251507556562No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2060.14068251507556562No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA2050.13999959024510172No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2000.13658496609278217No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC1770.12087769499211222No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA1740.1188289205007205No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT1670.11404844668747312No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1610.10995089770468965No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA1610.10995089770468965No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA1580.10790212321329792No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA1550.10585334872190617No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1530.10448749906097836No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT1530.10448749906097836No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA1500.10243872456958664No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1490.10175579973912272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCTAT200.00372434532.032491
CCCCGTG200.003743104931.99965731
CCCCCGT200.003743104931.99965730
GCCAAGA200.003743104931.99965730
GTACGCA200.003743104931.99965728
GAACCGC302.2383969E-531.988736
AGAACCG356.408361E-527.418915
AATAACG308.3487463E-426.6846162
ATATCAA451.2220902E-524.9056422
AAAAAGT601.7245475E-724.0243681
GGGGGTA350.002058117422.85697
AGAATAC350.00206221222.849095
GGGTAGG453.4923945E-421.3549941
GATATCA453.4923945E-421.3549941
TCTAATA400.00444776520.0134622
CATGTAA400.004465408619.99978632
TGGGGGT507.171965E-419.193246
AAAAGTA802.7447968E-618.0121152
AGTACTC802.771836E-617.993665
GTCCTAA450.00876303817.7958281
Produced by FastQC (version 0.11.5)