##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062985_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146429
Sequences flagged as poor quality	0
Sequence length	38
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.500160487335158	32.0	32.0	32.0	32.0	32.0
2	30.362216500829753	32.0	32.0	32.0	21.0	32.0
3	30.579939766029952	32.0	32.0	32.0	32.0	32.0
4	30.430454349889708	32.0	32.0	32.0	21.0	32.0
5	30.647597128984014	32.0	32.0	32.0	32.0	32.0
6	34.1408737340281	36.0	36.0	36.0	32.0	36.0
7	33.775911875379876	36.0	36.0	36.0	32.0	36.0
8	33.67565168101947	36.0	36.0	36.0	27.0	36.0
9	33.60917577802211	36.0	36.0	36.0	27.0	36.0
10	33.631527907723196	36.0	36.0	36.0	27.0	36.0
11	34.01279801132289	36.0	36.0	36.0	32.0	36.0
12	33.853936037260375	36.0	36.0	36.0	32.0	36.0
13	33.898653955159155	36.0	36.0	36.0	32.0	36.0
14	33.81076152947845	36.0	36.0	36.0	32.0	36.0
15	33.907135881553515	36.0	36.0	36.0	32.0	36.0
16	33.84006583395366	36.0	36.0	36.0	32.0	36.0
17	33.74926414849517	36.0	36.0	36.0	32.0	36.0
18	33.82325905387594	36.0	36.0	36.0	32.0	36.0
19	33.73297639128861	36.0	36.0	36.0	32.0	36.0
20	33.47054203743794	36.0	36.0	36.0	21.0	36.0
21	33.34179704839888	36.0	36.0	36.0	21.0	36.0
22	33.38794910844163	36.0	36.0	36.0	21.0	36.0
23	33.40164858054074	36.0	36.0	36.0	21.0	36.0
24	33.427688504326326	36.0	36.0	36.0	21.0	36.0
25	33.38316863462838	36.0	36.0	36.0	21.0	36.0
26	33.348687759938265	36.0	36.0	36.0	21.0	36.0
27	33.32770830914641	36.0	36.0	36.0	21.0	36.0
28	33.3449111856258	36.0	36.0	36.0	21.0	36.0
29	33.221527156505886	36.0	36.0	36.0	21.0	36.0
30	33.10036263308498	36.0	36.0	36.0	21.0	36.0
31	33.13577228554453	36.0	36.0	36.0	21.0	36.0
32	32.96278742598802	36.0	36.0	36.0	14.0	36.0
33	32.79885132043516	36.0	36.0	36.0	14.0	36.0
34	32.653244917331946	36.0	36.0	36.0	14.0	36.0
35	32.44600454827937	36.0	36.0	36.0	14.0	36.0
36	32.38069644674211	36.0	36.0	36.0	14.0	36.0
37	32.29595230452984	36.0	36.0	36.0	14.0	36.0
38	31.454786961599137	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	107.0
15	179.0
16	182.0
17	256.0
18	312.0
19	402.0
20	513.0
21	610.0
22	778.0
23	1013.0
24	1271.0
25	1568.0
26	2215.0
27	2958.0
28	4123.0
29	5549.0
30	7244.0
31	9045.0
32	11430.0
33	15483.0
34	28764.0
35	52426.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.86003104040038	16.145110453230867	13.157300989327297	18.837557517041454
2	13.840771338567611	22.264816219429694	39.42723601402186	24.46717642798084
3	19.526388765564487	33.14732219087885	26.383984372331927	20.942304671224736
4	11.009731944681578	16.621478572648112	33.688236298446306	38.680553184224
5	11.457508126860983	41.49684486573606	29.836506679050455	17.2091403283525
6	28.60723358920425	34.7180867047286	20.55617778020597	16.118501925861175
7	27.191335049751075	34.26711921818765	20.816231757370467	17.725313974690806
8	33.11365917953411	26.609483094195824	19.82735660285872	20.449501123411345
9	31.848882393514945	13.661911233430537	19.067944191382853	35.421262181671665
10	18.508628755232913	27.398944198212106	28.29630742544168	25.796119621113306
11	33.97779113284344	24.571120277542548	20.14710301308493	21.30398557652908
12	26.522249842926215	27.002349277460596	27.592400360586772	18.883000519026417
13	32.67034588273865	21.98718649254139	25.79367247691383	19.548795147806132
14	27.20877499726806	19.370833788656977	28.24008305103267	25.18030816304229
15	26.329091443130665	24.08450992848214	31.121539375806883	18.46485925258031
16	22.419834033398217	27.138612847044357	27.848922583068674	22.59263053648875
17	19.983880111746345	25.431855904592123	29.42562652404664	25.158637459614898
18	22.50472931904635	23.41030001297575	33.866704910979536	20.21826575699837
19	21.742396820084824	27.74366714702327	32.355773499340934	18.15816253355097
20	24.7452533806857	20.681600874197514	34.8702363065155	19.702909438601285
21	26.665391812705742	22.95146904153747	28.488888281815573	21.89425086394121
22	23.481925159986066	24.86289347693947	29.683989099775303	21.971192263299162
23	21.19372199759589	25.860561687247298	30.321822751611844	22.623893563544968
24	20.213783211529268	29.17150467864217	30.613346082917836	20.00136602691073
25	23.666218145307262	25.030906992152012	29.08740702288825	22.215467839652476
26	20.173766931005513	25.790455113624724	31.69197352513268	22.343804430237086
27	22.113020434925147	28.504398426401483	30.902360397770735	18.480220740902634
28	20.27905642594692	26.26517872997227	31.701520263348403	21.754244580732404
29	18.74031020988	28.907845400465792	33.238626662204524	19.11321772744968
30	21.676865080449094	27.49679023137651	31.94812467560849	18.8782200125659
31	18.91151723761132	29.13798284434246	29.877615691416707	22.07288422662951
32	18.077448436006012	30.632427264034966	30.062832946318807	21.22729135364021
33	19.41154776052125	29.528473275143767	29.497739348987146	21.56223961534784
34	18.163687650686754	30.786883678361892	31.10174642962032	19.94768224133103
35	20.868537706530425	29.887232167860827	29.484245971845606	19.759984153763142
36	20.431943609638818	32.157395770722914	28.525763619474347	18.884897000163924
37	21.01136487815539	32.76827669107201	28.473664080428367	17.746694350344224
38	19.31322264944715	33.70713617396174	26.987563429242673	19.992077747348436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	168.0
2	219.0
3	219.0
4	536.5
5	854.0
6	854.0
7	1099.0
8	1344.0
9	1404.0
10	1464.0
11	1464.0
12	1746.0
13	2028.0
14	2332.5
15	2637.0
16	2637.0
17	3673.5
18	4710.0
19	4710.0
20	5065.5
21	5421.0
22	4190.0
23	2959.0
24	2959.0
25	2439.0
26	1919.0
27	1919.0
28	2712.0
29	3505.0
30	4445.5
31	5386.0
32	5386.0
33	4769.5
34	4153.0
35	4153.0
36	4160.0
37	4167.0
38	4944.5
39	5722.0
40	5722.0
41	6755.5
42	7789.0
43	9695.5
44	11602.0
45	11602.0
46	15630.0
47	19658.0
48	19658.0
49	17084.0
50	14510.0
51	11619.5
52	8729.0
53	8729.0
54	7824.5
55	6920.0
56	6920.0
57	7014.5
58	7109.0
59	6520.0
60	5931.0
61	5931.0
62	5540.5
63	5150.0
64	4579.5
65	4009.0
66	4009.0
67	3589.0
68	3169.0
69	3169.0
70	2629.0
71	2089.0
72	1697.5
73	1306.0
74	1306.0
75	1060.0
76	814.0
77	814.0
78	620.5
79	427.0
80	325.5
81	224.0
82	224.0
83	180.5
84	137.0
85	137.0
86	97.5
87	58.0
88	44.0
89	30.0
90	30.0
91	25.0
92	20.0
93	18.0
94	16.0
95	16.0
96	12.0
97	8.0
98	8.0
99	63.5
100	119.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11473137151793703
2	0.05873153541989633
3	0.015024346270206039
4	0.002731699321855643
5	6.829248304639108E-4
6	6.829248304639108E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	6.829248304639108E-4
12	6.829248304639108E-4
13	0.014341421439742128
14	0.008878022796030841
15	0.021853594574845144
16	0.009560947626494752
17	0.017756045592061682
18	0.0013658496609278215
19	0.006829248304639109
20	0.006146323474175198
21	0.004780473813247376
22	0.006829248304639109
23	0.008878022796030841
24	0.012975571778814306
25	0.015024346270206039
26	0.017756045592061682
27	0.008878022796030841
28	0.004780473813247376
29	0.00819509796556693
30	6.829248304639108E-4
31	0.0034146241523195543
32	0.006146323474175198
33	0.0075121731351030195
34	0.010926797287422572
35	0.015024346270206039
36	0.014341421439742128
37	0.008878022796030841
38	0.004097548982783465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	146429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.80953909403192
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48427799253643	29.988595155331254
2	13.223555399367438	10.792944020651648
3	5.261308298609368	6.4413470009356075
4	2.4599628495406396	4.015598003127796
5	1.4190806098030357	2.895601281166982
6	0.8584768311662232	2.1020426281679176
7	0.6007664374048228	1.7161900989558079
8	0.4083203641414395	1.333069269065554
9	0.27946516726073933	1.026436020187258
>10	1.748749100523788	13.337521938960178
>50	0.12550830865003262	3.4754044622308427
>100	0.1054269792660274	8.354219451065022
>500	0.013387552922670149	3.92203730135424
>1k	0.01171410880733638	10.598993368799896
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3980	2.718040825246365	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2717	1.8555067643704455	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2320	1.5843856066762732	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2000	1.3658496609278217	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1795	1.22585007068272	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1491	1.018240922221691	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1217	0.8311195186745794	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	956	0.6528761379234987	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	932	0.636485941992365	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	810	0.5531691126757678	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	747	0.5101448483565414	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	669	0.45687671158035636	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	605	0.413169522430666	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	513	0.35034043802798626	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	511	0.34897458836705847	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	459	0.31346249718293506	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	434	0.29638937642133734	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	410	0.27999918049020345	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	391	0.26702360871138914	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	353	0.24107246515376052	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	349	0.23834076583190486	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	345	0.23560906651004923	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	345	0.23560906651004923	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	317	0.2164871712570597	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	313	0.21375547193520408	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	299	0.20419452430870932	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	296	0.20214574981731762	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	285	0.1946335766822146	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	284	0.19395065185175067	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	270	0.1843897042252559	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	263	0.17960923041200855	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	241	0.16458488414180253	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	225	0.15365808685437995	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	211	0.1440971392278852	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	206	0.14068251507556562	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	206	0.14068251507556562	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	205	0.13999959024510172	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	200	0.13658496609278217	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	177	0.12087769499211222	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	174	0.1188289205007205	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	167	0.11404844668747312	No Hit
GGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	161	0.10995089770468965	No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA	161	0.10995089770468965	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	158	0.10790212321329792	No Hit
GAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA	155	0.10585334872190617	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	153	0.10448749906097836	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	153	0.10448749906097836	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	150	0.10243872456958664	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	149	0.10175579973912272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.829248304639109E-4	0.0
5	0.0	0.0	0.0	6.829248304639109E-4	0.0
6	0.0	0.0	0.0	6.829248304639109E-4	0.0
7	0.0	0.0	0.0	6.829248304639109E-4	0.0
8	0.0	0.0	0.0	6.829248304639109E-4	0.0
9	0.0	0.0	0.0	6.829248304639109E-4	0.0
10	0.0	0.0	0.0	6.829248304639109E-4	0.0
11	0.0	0.0	0.0	6.829248304639109E-4	0.0
12	0.0	0.0	0.0	6.829248304639109E-4	0.0
13	0.0	0.0	0.0	6.829248304639109E-4	0.0
14	0.0	0.0	0.0	6.829248304639109E-4	0.0
15	0.0	0.0	0.0	6.829248304639109E-4	0.0
16	0.0	0.0	0.0	6.829248304639109E-4	0.0
17	0.0	0.0	0.0	6.829248304639109E-4	0.0
18	0.0	0.0	0.0	6.829248304639109E-4	0.0
19	0.0	0.0	0.0	6.829248304639109E-4	0.0
20	0.0	0.0	0.0	0.0013658496609278217	0.0
21	6.829248304639109E-4	0.0	0.0	0.0020487744913917326	0.0
22	6.829248304639109E-4	0.0	0.0	0.006829248304639109	0.0
23	6.829248304639109E-4	0.0	0.0	0.011609722117886485	0.0
24	6.829248304639109E-4	0.0	0.0	0.02390236906623688	0.0
25	6.829248304639109E-4	0.0	0.0	0.030048692540412078	0.0
26	6.829248304639109E-4	0.0	0.0	0.04097548982783465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCTAT	20	0.003724345	32.03249	1
CCCCGTG	20	0.0037431049	31.999657	31
CCCCCGT	20	0.0037431049	31.999657	30
GCCAAGA	20	0.0037431049	31.999657	30
GTACGCA	20	0.0037431049	31.999657	28
GAACCGC	30	2.2383969E-5	31.98873	6
AGAACCG	35	6.408361E-5	27.41891	5
AATAACG	30	8.3487463E-4	26.684616	2
ATATCAA	45	1.2220902E-5	24.905642	2
AAAAAGT	60	1.7245475E-7	24.024368	1
GGGGGTA	35	0.0020581174	22.8569	7
AGAATAC	35	0.002062212	22.84909	5
GGGTAGG	45	3.4923945E-4	21.354994	1
GATATCA	45	3.4923945E-4	21.354994	1
TCTAATA	40	0.004447765	20.013462	2
CATGTAA	40	0.0044654086	19.999786	32
TGGGGGT	50	7.171965E-4	19.19324	6
AAAAGTA	80	2.7447968E-6	18.012115	2
AGTACTC	80	2.771836E-6	17.99366	5
GTCCTAA	45	0.008763038	17.795828	1
>>END_MODULE