FastQCFastQC Report
Thu 2 Feb 2017
SRR4062985_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062985_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146429
Sequences flagged as poor quality0
Sequence length38
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT50053.418038776471874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT36682.5049682781416247No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA25601.7482875659876118No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC22961.5679954107451395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT22111.5099468001557068No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16281.1118016239952468No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA14170.9677044847673616No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG10330.7054613498692199No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA7420.5067302242042219No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA7250.4951205020863354No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7240.4944375772558714No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC6260.42751094387040817No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT6220.42477924454855254No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA6130.41863292107437733No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA6060.4138524472611299No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5970.40770612378695475No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.39746225132999613No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA5610.383120829890254No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG5440.3715111077723675No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC4780.3264380689617494No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4710.321657595148502No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA4620.3155112716743268No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4160.2840967294729869No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT3590.245170014136544No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC3580.24448708930608007No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3570.24380416447561618No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA3560.24312123964515225No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC3200.21853594574845148No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2810.19190187736035894No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA2790.19053602769943112No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA2780.1898531028689672No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA2750.1878043283775755No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG2560.17482875659876115No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA2540.17346290693783334No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2300.15707271100669948No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT2280.15570686134577166No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT2270.15502393651530777No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2200.15024346270206038No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2190.14956053787159648No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA2120.1447800640583491No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT2100.14341421439742127No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2050.13999959024510172No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2050.13999959024510172No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC1950.13317034194046262No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA1930.13180449227953478No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA1930.13180449227953478No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1910.13043864261860696No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1890.12907279295767915No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA1840.1256581688053596No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG1840.1256581688053596No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1730.11814599567025659No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA1730.11814599567025659No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1670.11404844668747312No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT1670.11404844668747312No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA1620.11063382253515355No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA1580.10790212321329792No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT1530.10448749906097836No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1520.10380457423051445No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT1480.10107287490865881No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCTTC252.8829923E-431.99965916
CTCACGA200.003743109331.99965726
GCCTAGC200.003743109331.99965712
TCTCACG200.003743109331.99965725
GGCCTAG200.003743109331.99965711
TAATACT308.3821814E-426.6663824
CTACGAC308.3821814E-426.6663824
TGTTCCT308.3821814E-426.66638232
GAACCGC308.3821814E-426.6663826
AATACTT308.3821814E-426.6663825
CTAATAC308.3821814E-426.6663823
CCCTGGA308.3821814E-426.6663822
TCCTAAG350.002058119722.85692
ATAGAAC555.7893638E-520.3634193
GTCACTG400.00446541619.99978630
TTGTTCC400.00446541619.99978631
GGTAGCA400.00446541619.99978617
AAAAAGT601.1267228E-418.6728461
TACTTGT450.00881501617.7775887
ACTTGTA450.00881501617.7775888
Produced by FastQC (version 0.11.5)