##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062985_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	146429
Sequences flagged as poor quality	0
Sequence length	38
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.240594417772435	32.0	32.0	32.0	21.0	32.0
2	31.18797505958519	32.0	32.0	32.0	32.0	32.0
3	31.342684850678484	32.0	32.0	32.0	32.0	32.0
4	31.512944840161442	32.0	32.0	32.0	32.0	32.0
5	31.316146391766658	32.0	32.0	32.0	32.0	32.0
6	34.796932301661556	36.0	36.0	36.0	32.0	36.0
7	34.86973891783732	36.0	36.0	36.0	32.0	36.0
8	34.87577597333861	36.0	36.0	36.0	32.0	36.0
9	34.87557109588948	36.0	36.0	36.0	32.0	36.0
10	34.78001625361097	36.0	36.0	36.0	32.0	36.0
11	34.95300794241578	36.0	36.0	36.0	36.0	36.0
12	34.84315265418736	36.0	36.0	36.0	32.0	36.0
13	34.86227454944034	36.0	36.0	36.0	32.0	36.0
14	34.77326895628598	36.0	36.0	36.0	32.0	36.0
15	34.783034781361614	36.0	36.0	36.0	32.0	36.0
16	34.73866515512638	36.0	36.0	36.0	32.0	36.0
17	34.679762888498864	36.0	36.0	36.0	32.0	36.0
18	34.63166449268929	36.0	36.0	36.0	32.0	36.0
19	34.59515533125269	36.0	36.0	36.0	32.0	36.0
20	34.50973509345826	36.0	36.0	36.0	32.0	36.0
21	34.43753628038162	36.0	36.0	36.0	32.0	36.0
22	34.44253529014061	36.0	36.0	36.0	32.0	36.0
23	34.34978043966701	36.0	36.0	36.0	32.0	36.0
24	34.335343408751	36.0	36.0	36.0	32.0	36.0
25	34.321958082073905	36.0	36.0	36.0	32.0	36.0
26	34.22813103961647	36.0	36.0	36.0	32.0	36.0
27	34.16564341762902	36.0	36.0	36.0	32.0	36.0
28	34.07547002301457	36.0	36.0	36.0	32.0	36.0
29	33.99963804983985	36.0	36.0	36.0	32.0	36.0
30	33.94288699642831	36.0	36.0	36.0	32.0	36.0
31	33.87070867109657	36.0	36.0	36.0	32.0	36.0
32	33.744879771083596	36.0	36.0	36.0	32.0	36.0
33	33.52844040456467	36.0	36.0	36.0	21.0	36.0
34	33.34239119300138	36.0	36.0	36.0	21.0	36.0
35	33.1302815699076	36.0	36.0	36.0	14.0	36.0
36	33.04596767033853	36.0	36.0	36.0	14.0	36.0
37	32.91676512166306	36.0	36.0	36.0	14.0	36.0
38	32.51706970613745	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	10.0
22	8.0
23	42.0
24	127.0
25	369.0
26	751.0
27	1591.0
28	2833.0
29	4287.0
30	6422.0
31	8900.0
32	11636.0
33	16785.0
34	32580.0
35	60086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.00288208056167	17.71864883692341	13.756129543374628	19.522339539140294
2	13.61547234495899	23.498077566602245	37.90984026388215	24.97660982455661
3	19.230480301033264	32.381563761276794	27.39279787473793	20.99515806295201
4	10.96640692758948	17.10248652930772	33.319902478334214	38.611204064768586
5	11.114601615800149	41.03285551359362	30.34781361615527	17.50472925445096
6	28.948500638534718	34.93638555204229	19.247553421794862	16.86756038762813
7	26.206736416532355	33.94159586964241	21.393449340290108	18.458218373535114
8	32.60804239742119	26.841228213954	20.153116975359232	20.397612413265584
9	30.433951182199397	13.11346655557225	19.709469888404747	36.74311237382361
10	18.50000341467079	27.264780402521392	28.779528365670267	25.45568781713755
11	34.031320643896706	23.965141612200437	20.447886573647224	21.555651170255633
12	25.572803201551604	27.34909068558824	28.29152695162843	18.786579161231725
13	32.589172909737826	21.723838857057004	26.42919093895335	19.257797294251823
14	26.71943399190051	19.21750472925445	28.94030554056915	25.122755738275888
15	26.12665523905784	23.584126095240695	31.56137103988964	18.727847625811826
16	22.38438003660502	26.15893135192723	28.708307154369383	22.74838145709837
17	20.14081910004166	24.55046473034713	30.809470801548873	24.499245368062336
18	22.276325044902308	21.11193820896134	36.01335800968388	20.598378736452478
19	21.643936651892727	26.162850255072424	34.6181425810461	17.575070511988745
20	23.83271073352956	19.581503663891716	37.51784141119587	19.067944191382853
21	25.63494936112382	21.71974130807422	31.224689098470932	21.42062023233103
22	22.4921292913289	23.52402871015987	32.439612371866225	21.544229626644995
23	20.644817624924023	24.019832137076673	32.926537775987	22.408812462012307
24	19.72355202862821	26.95367720874963	33.14439079690498	20.178379965717173
25	23.25768802627895	22.939445055282764	31.766248489028815	22.036618429409476
26	19.8164298055713	23.382663270253843	34.297167910728064	22.50373901344679
27	22.233983705413547	25.8794364504299	33.51931652882968	18.36726331532688
28	20.4693059434948	23.14090788026962	34.49863073571492	21.891155440520663
29	18.778384063266156	26.024216514488252	36.06184567264681	19.13555374959878
30	21.879545718402778	24.55387935449945	34.6939472372276	18.872627689870175
31	18.94501772189935	26.10070409550021	32.98800101072875	21.966277171871692
32	18.369995014648737	27.46791960608896	33.076098313858594	21.08598706540371
33	19.472918615847952	26.290557198369175	32.5461486454186	21.690375540364272
34	18.662969766917755	27.498651223459834	34.1489732225174	19.689405787105013
35	21.59408313926886	26.640214711566696	32.11932062637865	19.646381522785784
36	20.588817788825985	28.28333185366287	31.76010216555464	19.36774819195651
37	21.843350702388186	28.891134952775747	31.20010380457423	18.065410540261833
38	19.99945366013563	29.477084457313786	30.09854605303594	20.424915829514646
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	47.0
1	65.5
2	84.0
3	84.0
4	442.5
5	801.0
6	801.0
7	1126.5
8	1452.0
9	1551.0
10	1650.0
11	1650.0
12	2013.0
13	2376.0
14	2698.0
15	3020.0
16	3020.0
17	4366.5
18	5713.0
19	5713.0
20	5922.5
21	6132.0
22	4771.0
23	3410.0
24	3410.0
25	2508.5
26	1607.0
27	1607.0
28	2352.5
29	3098.0
30	3780.5
31	4463.0
32	4463.0
33	4024.0
34	3585.0
35	3585.0
36	3601.5
37	3618.0
38	4300.0
39	4982.0
40	4982.0
41	5829.5
42	6677.0
43	8364.5
44	10052.0
45	10052.0
46	15477.0
47	20902.0
48	20902.0
49	18477.0
50	16052.0
51	12674.5
52	9297.0
53	9297.0
54	8176.5
55	7056.0
56	7056.0
57	6994.5
58	6933.0
59	6455.0
60	5977.0
61	5977.0
62	5554.5
63	5132.0
64	4545.5
65	3959.0
66	3959.0
67	3559.0
68	3159.0
69	3159.0
70	2708.5
71	2258.0
72	1787.5
73	1317.0
74	1317.0
75	1031.0
76	745.0
77	745.0
78	588.0
79	431.0
80	327.5
81	224.0
82	224.0
83	184.5
84	145.0
85	145.0
86	98.0
87	51.0
88	33.5
89	16.0
90	16.0
91	9.5
92	3.0
93	2.5
94	2.0
95	2.0
96	1.5
97	1.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.004780473813247376
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	6.829248304639108E-4
8	0.0034146241523195543
9	0.004780473813247376
10	0.0013658496609278215
11	0.005463398643711286
12	0.0
13	0.0
14	0.0
15	0.0
16	6.829248304639108E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	146429.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.94145285428433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.8982677980515	26.190850173121444
2	14.582293457563825	10.773822125398658
3	5.643983509881131	6.254908522218959
4	2.6398979535244855	3.900866631609859
5	1.5306971327158783	2.827308798120591
6	0.9502153698260404	2.106140177150701
7	0.6544284842770783	1.6922877298895709
8	0.5102323775719594	1.507898025664315
9	0.36788493890152146	1.2231183713608642
>10	1.933706764276339	13.256253884134972
>50	0.153439446878524	4.064768590921197
>100	0.09982807387277466	7.926708507194613
>500	0.020335348381491137	4.740864173080469
>1k	0.012940676242767088	10.11616551366191
>5k	0.0018486680346810123	3.418038776471874
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5005	3.418038776471874	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3668	2.5049682781416247	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	2560	1.7482875659876118	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	2296	1.5679954107451395	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2211	1.5099468001557068	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1628	1.1118016239952468	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	1417	0.9677044847673616	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	1033	0.7054613498692199	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAA	742	0.5067302242042219	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA	725	0.4951205020863354	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	724	0.4944375772558714	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC	626	0.42751094387040817	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	622	0.42477924454855254	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAA	613	0.41863292107437733	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	606	0.4138524472611299	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	597	0.40770612378695475	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.39746225132999613	No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA	561	0.383120829890254	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATG	544	0.3715111077723675	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC	478	0.3264380689617494	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	471	0.321657595148502	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAA	462	0.3155112716743268	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	416	0.2840967294729869	No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGT	359	0.245170014136544	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC	358	0.24448708930608007	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG	357	0.24380416447561618	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAA	356	0.24312123964515225	No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC	320	0.21853594574845148	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	281	0.19190187736035894	No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	279	0.19053602769943112	No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAA	278	0.1898531028689672	No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAA	275	0.1878043283775755	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	256	0.17482875659876115	No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAA	254	0.17346290693783334	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT	230	0.15707271100669948	No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT	228	0.15570686134577166	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	227	0.15502393651530777	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	220	0.15024346270206038	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	219	0.14956053787159648	No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAA	212	0.1447800640583491	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT	210	0.14341421439742127	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	205	0.13999959024510172	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	205	0.13999959024510172	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	195	0.13317034194046262	No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAA	193	0.13180449227953478	No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAA	193	0.13180449227953478	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	191	0.13043864261860696	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	189	0.12907279295767915	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAA	184	0.1256581688053596	No Hit
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTG	184	0.1256581688053596	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	173	0.11814599567025659	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	173	0.11814599567025659	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT	167	0.11404844668747312	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT	167	0.11404844668747312	No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAA	162	0.11063382253515355	No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCA	158	0.10790212321329792	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACT	153	0.10448749906097836	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT	152	0.10380457423051445	No Hit
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT	148	0.10107287490865881	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.829248304639109E-4	0.0
5	0.0	0.0	0.0	6.829248304639109E-4	0.0
6	0.0	0.0	0.0	6.829248304639109E-4	0.0
7	0.0	0.0	0.0	6.829248304639109E-4	0.0
8	0.0	0.0	0.0	6.829248304639109E-4	0.0
9	0.0	0.0	0.0	6.829248304639109E-4	0.0
10	0.0	0.0	0.0	6.829248304639109E-4	0.0
11	0.0	0.0	0.0	6.829248304639109E-4	0.0
12	0.0	0.0	0.0	0.0013658496609278217	0.0
13	0.0	0.0	0.0	0.0013658496609278217	0.0
14	0.0	0.0	0.0	0.0013658496609278217	0.0
15	0.0	0.0	0.0	0.0013658496609278217	0.0
16	0.0	0.0	0.0	0.0013658496609278217	0.0
17	0.0	0.0	0.0	0.0013658496609278217	0.0
18	0.0	0.0	0.0	0.0013658496609278217	0.0
19	0.0	0.0	0.0	0.0013658496609278217	0.0
20	0.0	0.0	0.0	0.0027316993218556435	0.0
21	0.0	0.0	0.0	0.0034146241523195543	0.0
22	0.0	0.0	0.0	0.00819509796556693	0.0
23	0.0	0.0	0.0	0.012292646948350396	0.0
24	0.0	0.0	0.0	0.02390236906623688	0.0
25	0.0	0.0	0.0	0.030048692540412078	0.0
26	0.0	0.0	0.0	0.04097548982783465	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCTTC	25	2.8829923E-4	31.999659	16
CTCACGA	20	0.0037431093	31.999657	26
GCCTAGC	20	0.0037431093	31.999657	12
TCTCACG	20	0.0037431093	31.999657	25
GGCCTAG	20	0.0037431093	31.999657	11
TAATACT	30	8.3821814E-4	26.666382	4
CTACGAC	30	8.3821814E-4	26.666382	4
TGTTCCT	30	8.3821814E-4	26.666382	32
GAACCGC	30	8.3821814E-4	26.666382	6
AATACTT	30	8.3821814E-4	26.666382	5
CTAATAC	30	8.3821814E-4	26.666382	3
CCCTGGA	30	8.3821814E-4	26.666382	2
TCCTAAG	35	0.0020581197	22.8569	2
ATAGAAC	55	5.7893638E-5	20.363419	3
GTCACTG	40	0.004465416	19.999786	30
TTGTTCC	40	0.004465416	19.999786	31
GGTAGCA	40	0.004465416	19.999786	17
AAAAAGT	60	1.1267228E-4	18.672846	1
TACTTGT	45	0.008815016	17.777588	7
ACTTGTA	45	0.008815016	17.777588	8
>>END_MODULE