FastQCFastQC Report
Thu 2 Feb 2017
SRR4062984_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062984_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137224
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32150.2827059576653324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22000.19345353246150274No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20430.17964798491765913No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19250.1692718409038149No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT17400.15300415749227944No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15370.13515367245151352No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14960.13154840207382187No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14720.12943799990151456No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC14720.12943799990151456No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14640.12873453251074546No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13900.12222745914613128No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13800.12134812490766991No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13240.1164238531722862No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13190.11598418605305551No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13130.11545658550997867No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12320.10833397817844154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12060.10604770915844194No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12040.10587184231074968No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11650.10244243878075032No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG11630.10226657193305805No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11620.1021786385092119No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11520.10129930427075053No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTGC659.441284E-619.6904688
TAGCGGC4000.018.39909230
GATATAC2450.018.3033221
GGACCGT701.7907036E-518.2815936
AAGACGG5250.017.97695
CTAGCGG4100.017.9511229
CCTATTC4350.017.284983
GACGGAC5300.017.2059387
GCGCAAG5400.017.2017351
TCGTTTA2700.017.18433630
TCTAGCG4300.017.11543328
CGCAAGA5350.017.0518842
GTCCTAT4150.016.980191
CGTTTAT2800.016.57060831
ATCGTTT2900.016.55163229
AACGAAT1456.91216E-1116.55090531
CTATTCC4350.016.5472664
CGCGGTC1750.016.45777510
TCGCGTA2150.016.3727239
CAAGACG5600.016.2813284
Produced by FastQC (version 0.11.5)