##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062984_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137224
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265898363031383	32.0	32.0	32.0	32.0	32.0
2	30.918573649518475	32.0	32.0	32.0	32.0	32.0
3	30.973975223878497	32.0	32.0	32.0	32.0	32.0
4	31.037489535922564	32.0	32.0	32.0	32.0	32.0
5	30.95130774587944	32.0	32.0	32.0	32.0	32.0
6	34.59813282167805	36.0	36.0	36.0	32.0	36.0
7	34.53921742770114	36.0	36.0	36.0	32.0	36.0
8	34.5168612340225	36.0	36.0	36.0	32.0	36.0
9	34.64454320344981	36.0	36.0	36.0	32.0	36.0
10	34.39337280957841	36.0	36.0	36.0	32.0	36.0
11	34.62999813581141	36.0	36.0	36.0	32.0	36.0
12	34.46534104099104	36.0	36.0	36.0	32.0	36.0
13	34.561354667154404	36.0	36.0	36.0	32.0	36.0
14	34.46101999254324	36.0	36.0	36.0	32.0	36.0
15	34.411332332064745	36.0	36.0	36.0	32.0	36.0
16	34.42136641505983	36.0	36.0	36.0	32.0	36.0
17	34.345406006204584	36.0	36.0	36.0	32.0	36.0
18	34.37359482388694	36.0	36.0	36.0	32.0	36.0
19	34.35716798097824	36.0	36.0	36.0	32.0	36.0
20	34.33157935463902	36.0	36.0	36.0	32.0	36.0
21	34.31460292783128	36.0	36.0	36.0	32.0	36.0
22	34.30020382967648	36.0	36.0	36.0	32.0	36.0
23	34.249816219144165	36.0	36.0	36.0	32.0	36.0
24	34.241721068144884	36.0	36.0	36.0	32.0	36.0
25	34.21447929343735	36.0	36.0	36.0	32.0	36.0
26	34.168422404029464	36.0	36.0	36.0	32.0	36.0
27	34.17317608492258	36.0	36.0	36.0	32.0	36.0
28	34.162830717607086	36.0	36.0	36.0	32.0	36.0
29	34.12553639388546	36.0	36.0	36.0	32.0	36.0
30	34.092211384916254	36.0	36.0	36.0	32.0	36.0
31	34.122259115178714	36.0	36.0	36.0	32.0	36.0
32	34.0668874381828	36.0	36.0	36.0	32.0	36.0
33	34.019467580705296	36.0	36.0	36.0	32.0	36.0
34	34.028806989652	36.0	36.0	36.0	32.0	36.0
35	33.98838751204688	36.0	36.0	36.0	32.0	36.0
36	33.956706858103594	36.0	36.0	36.0	32.0	36.0
37	33.95401257799695	36.0	36.0	36.0	32.0	36.0
38	33.33330900508607	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	1.0
10	10.0
11	11.0
12	7.0
13	5.0
14	87.0
15	285.0
16	347.0
17	420.0
18	516.0
19	639.0
20	915.0
21	1353.0
22	1982.0
23	3005.0
24	4416.0
25	6585.0
26	9862.0
27	14363.0
28	20396.0
29	27958.0
30	38419.0
31	52029.0
32	72346.0
33	111214.0
34	249912.0
35	520138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13536606622522	17.124201763822448	11.183757621427855	25.556674548524484
2	17.006816486254415	19.3961937472727	37.20729930603454	26.389690460438338
3	19.463789617541604	23.26401317044986	28.100917164941063	29.171280047067473
4	12.3783963932434	14.86432893451366	35.7571542008039	37.00012047143903
5	14.624070431340963	36.46863714264861	33.42103249939986	15.486259926610572
6	34.92188413685643	35.420555898985334	16.403320023108964	13.254239941049278
7	30.718926086681254	30.19308421208135	20.37294323721624	18.71504646402116
8	28.735123784533833	32.576691540232396	19.101350840384164	19.586833834849607
9	27.45752368490937	13.898077947841319	18.50579746082999	40.13860090641933
10	15.992845717762863	26.464864788814136	31.136164386693526	26.406125106729473
11	38.49904591060578	20.960332744172142	21.558814994592026	18.981806350630052
12	24.855631619906877	23.709302274456007	28.384226100185106	23.05084000545201
13	29.488783921524824	18.972572561304332	25.378757564378013	26.159885952792827
14	23.46496647246345	19.84768605034627	24.84106848411564	31.84627899307464
15	25.226212506068684	27.34251317520739	22.184305144135713	25.24696917458821
16	26.06577762456205	25.745050584643092	23.714101788402818	24.47507000239204
17	24.115822366772	25.867599165183986	24.987840990943848	25.028737477100165
18	25.153188106552793	24.52073755412588	26.34386948584373	23.982204853477597
19	25.57099389696959	24.74471041384526	25.30242538297835	24.3818703062068
20	25.767483498335324	24.23207680902742	24.89583681423338	25.10460287840388
21	27.31349084910074	24.036367921375785	24.368600262409025	24.281540967114452
22	25.833290096548474	24.156464983920312	25.33397118583405	24.67627373369717
23	24.38012422578736	23.87490183559738	25.825614291505843	25.919359647109413
24	24.670363253406737	25.13136660847694	25.40302615899427	24.795243979122052
25	24.83573851488797	24.583860643994726	25.383907079579863	25.196493761537443
26	24.595874845209952	25.816253658673872	25.777995891027807	23.809875605088372
27	25.489675332041777	24.533321138901325	25.037484687340335	24.939518841716566
28	24.355501932878635	24.7430449785607	25.970132716125377	24.931320372435287
29	24.15739032399338	24.922215909990307	25.9395210012646	24.980872764751716
30	24.139280400426312	25.328705543850273	26.09251944269549	24.439494613027925
31	24.97251930683579	25.22569343681903	24.694810413995544	25.10697684234964
32	24.71496699201248	24.994174026446842	24.59545758735223	25.69540139418845
33	24.214008764161736	24.49445138686748	25.509989614213314	25.78155023475747
34	25.26427061310782	24.8756477048471	25.37543136351324	24.484650318531834
35	25.65236624853899	24.608527482887784	25.73442047116537	24.00468579740786
36	24.15970802277774	25.62328782182354	25.06747576017413	25.14952839522459
37	25.441950615503405	25.299661072993302	24.93215303952624	24.32623527197705
38	24.44209746904792	24.915821066673935	25.472646353506672	25.169435110771477
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	83.5
2	121.0
3	121.0
4	320.5
5	520.0
6	520.0
7	684.5
8	849.0
9	932.5
10	1016.0
11	1016.0
12	1361.0
13	1706.0
14	2251.0
15	2796.0
16	2796.0
17	4070.0
18	5344.0
19	5344.0
20	6440.0
21	7536.0
22	8046.5
23	8557.0
24	8557.0
25	10254.5
26	11952.0
27	11952.0
28	15354.0
29	18756.0
30	22771.0
31	26786.0
32	26786.0
33	32990.5
34	39195.0
35	39195.0
36	43513.0
37	47831.0
38	55690.5
39	63550.0
40	63550.0
41	66380.0
42	69210.0
43	78402.0
44	87594.0
45	87594.0
46	89000.5
47	90407.0
48	90407.0
49	96577.5
50	102748.0
51	104880.0
52	107012.0
53	107012.0
54	101218.0
55	95424.0
56	95424.0
57	93219.0
58	91014.0
59	81575.5
60	72137.0
61	72137.0
62	68400.5
63	64664.0
64	54197.0
65	43730.0
66	43730.0
67	36841.0
68	29952.0
69	29952.0
70	23862.5
71	17773.0
72	13921.5
73	10070.0
74	10070.0
75	7686.0
76	5302.0
77	5302.0
78	5114.5
79	4927.0
80	3908.0
81	2889.0
82	2889.0
83	2880.5
84	2872.0
85	2872.0
86	1940.5
87	1009.0
88	857.5
89	706.0
90	706.0
91	503.0
92	300.0
93	257.5
94	215.0
95	215.0
96	190.5
97	166.0
98	166.0
99	354.0
100	542.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11273064937074842
2	0.049946184744606166
3	0.011695145371536302
4	0.0021983355961534405
5	2.6380027153841286E-4
6	2.6380027153841286E-4
7	0.0
8	5.276005430768257E-4
9	1.758668476922752E-4
10	2.6380027153841286E-4
11	0.0012310679338459266
12	0.0025500692915379907
13	0.013277947000766779
14	0.008705408960767623
15	0.021104021723073026
16	0.010552010861536513
17	0.018202218736150487
18	0.005276005430768256
19	0.007386407603075559
20	0.005803605973845083
21	0.005979472821537358
22	0.00659500678846032
23	0.008881275808459899
24	0.012222745914613128
25	0.014509014934612706
26	0.017586684769227524
27	0.008177808417690797
28	0.005276005430768256
29	0.008969209232306036
30	0.003165603258460954
31	0.005451872278460532
32	0.00659500678846032
33	0.008177808417690797
34	0.011607211947690165
35	0.015036615477689532
36	0.013102080153074505
37	0.009320942927690588
38	0.0055398057023066695
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1137224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.414090057888885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68052676426042	42.81329269152604
2	13.240206250873685	14.409075506401281
3	3.9600124140201607	6.464414163805531
4	1.5967526981682498	3.475433804732169
5	0.7302158681562931	1.9867016005778024
6	0.42930636424856666	1.4016189099987817
7	0.2586770108593481	0.9852971915364247
8	0.17536847663695931	0.7634012864830629
9	0.12350872453343544	0.6048553373727611
>10	0.6279717853350014	6.336825039745751
>50	0.07581079995320314	2.9054773825242726
>100	0.08635576113947684	10.319592613481932
>500	0.010570854446714496	3.9204980577749247
>1k	0.00471622736853416	3.6135164140392586
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3215	0.2827059576653324	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2200	0.19345353246150274	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2043	0.17964798491765913	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1925	0.1692718409038149	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1740	0.15300415749227944	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1537	0.13515367245151352	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1496	0.13154840207382187	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1472	0.12943799990151456	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1472	0.12943799990151456	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1464	0.12873453251074546	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1390	0.12222745914613128	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1380	0.12134812490766991	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1324	0.1164238531722862	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1319	0.11598418605305551	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1313	0.11545658550997867	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1232	0.10833397817844154	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1206	0.10604770915844194	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1204	0.10587184231074968	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1165	0.10244243878075032	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1163	0.10226657193305805	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1162	0.1021786385092119	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1152	0.10129930427075053	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.638002715384128E-4
2	0.0	0.0	0.0	0.0	2.638002715384128E-4
3	0.0	0.0	0.0	0.0	2.638002715384128E-4
4	0.0	0.0	0.0	0.0	2.638002715384128E-4
5	0.0	8.793342384613762E-5	0.0	0.0	2.638002715384128E-4
6	0.0	8.793342384613762E-5	0.0	0.0	3.5173369538455047E-4
7	0.0	8.793342384613762E-5	0.0	8.793342384613762E-5	3.5173369538455047E-4
8	0.0	8.793342384613762E-5	0.0	8.793342384613762E-5	3.5173369538455047E-4
9	0.0	8.793342384613762E-5	0.0	8.793342384613762E-5	3.5173369538455047E-4
10	0.0	8.793342384613762E-5	0.0	1.7586684769227523E-4	5.276005430768256E-4
11	0.0	8.793342384613762E-5	0.0	1.7586684769227523E-4	5.276005430768256E-4
12	0.0	8.793342384613762E-5	0.0	1.7586684769227523E-4	0.001143134509999789
13	0.0	8.793342384613762E-5	0.0	2.638002715384128E-4	0.0012310679338459266
14	0.0	8.793342384613762E-5	0.0	2.638002715384128E-4	0.0012310679338459266
15	0.0	8.793342384613762E-5	0.0	2.638002715384128E-4	0.001319001357692064
16	0.0	8.793342384613762E-5	0.0	3.5173369538455047E-4	0.001319001357692064
17	0.0	8.793342384613762E-5	0.0	4.3966711923068807E-4	0.0014948682053843394
18	0.0	8.793342384613762E-5	0.0	4.3966711923068807E-4	0.0017586684769227523
19	0.0	8.793342384613762E-5	0.0	5.276005430768256E-4	0.0017586684769227523
20	0.0	8.793342384613762E-5	0.0	5.276005430768256E-4	0.0018466019007688898
21	0.0	8.793342384613762E-5	0.0	6.155339669229633E-4	0.002022468748461165
22	0.0	8.793342384613762E-5	0.0	6.155339669229633E-4	0.002022468748461165
23	0.0	8.793342384613762E-5	0.0	9.672676623075137E-4	0.002022468748461165
24	0.0	8.793342384613762E-5	0.0	0.0021983355961534405	0.002022468748461165
25	0.0	8.793342384613762E-5	0.0	0.0028138695630764037	0.002022468748461165
26	0.0	8.793342384613762E-5	0.0	0.002989736410768679	0.0021104021723073025
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTGC	65	9.441284E-6	19.690468	8
TAGCGGC	400	0.0	18.399092	30
GATATAC	245	0.0	18.303322	1
GGACCGT	70	1.7907036E-5	18.281593	6
AAGACGG	525	0.0	17.9769	5
CTAGCGG	410	0.0	17.95112	29
CCTATTC	435	0.0	17.28498	3
GACGGAC	530	0.0	17.205938	7
GCGCAAG	540	0.0	17.201735	1
TCGTTTA	270	0.0	17.184336	30
TCTAGCG	430	0.0	17.115433	28
CGCAAGA	535	0.0	17.051884	2
GTCCTAT	415	0.0	16.98019	1
CGTTTAT	280	0.0	16.570608	31
ATCGTTT	290	0.0	16.551632	29
AACGAAT	145	6.91216E-11	16.550905	31
CTATTCC	435	0.0	16.547266	4
CGCGGTC	175	0.0	16.457775	10
TCGCGTA	215	0.0	16.372723	9
CAAGACG	560	0.0	16.281328	4
>>END_MODULE