FastQCFastQC Report
Thu 2 Feb 2017
SRR4062984_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062984_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137224
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT36690.32262773209147894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT26370.23188043868226488No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20740.1823739210568894No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT19760.1737564455199679No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT16950.14904715341920324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT16020.14086934500151244No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15640.13752787489535923No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15390.13532953929920577No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15260.13418640478920602No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA14880.13084493468305275No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14180.12468959501382314No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14050.12354646050382334No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13780.12117225805997762No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC13610.1196773898545933No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13130.11545658550997867No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12820.11273064937074842No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12570.11053231377459496No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG12260.10780637763536471No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12100.10639944285382652No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG12070.1061356425822881No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11680.10270623905228873No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG11440.10059583687998142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG2850.020.20966529
ACGAACG1052.8012437E-1019.8086815
TTACGCA507.189425E-419.2025594
TCTAGCG3000.019.19918328
TCGTTTA2950.018.98224330
CGAACGA1105.2023097E-1018.90828516
ATCGTTT3100.018.57985529
CATCGTT3150.018.28493528
TAGCGGC3100.018.06374730
CGGCATC3250.017.72232225
GCATCGT3400.016.94045427
GGCATCG3450.016.6949426
CGTTTAT3500.016.45644231
CGCATCG3600.016.44374513
TAGAACA2950.016.2733554
CCAGTCG3650.016.21848920
CAAGACG4200.016.0021324
CAGTCGG3700.015.99931921
TAACGAA1407.421477E-1015.99931813
TGTGTCG600.002442579315.99931810
Produced by FastQC (version 0.11.5)