##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1137224
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16622934443874	32.0	32.0	32.0	32.0	32.0
2	31.23698145659958	32.0	32.0	32.0	32.0	32.0
3	31.344623398732352	32.0	32.0	32.0	32.0	32.0
4	31.445042489430403	32.0	32.0	32.0	32.0	32.0
5	31.35168357333296	32.0	32.0	32.0	32.0	32.0
6	34.88376608302322	36.0	36.0	36.0	36.0	36.0
7	34.90883766083023	36.0	36.0	36.0	36.0	36.0
8	34.84528202007696	36.0	36.0	36.0	32.0	36.0
9	34.9698942336778	36.0	36.0	36.0	32.0	36.0
10	34.80450553277103	36.0	36.0	36.0	32.0	36.0
11	34.988909836584526	36.0	36.0	36.0	36.0	36.0
12	34.874362482677114	36.0	36.0	36.0	32.0	36.0
13	34.94404092773279	36.0	36.0	36.0	36.0	36.0
14	34.892091619592975	36.0	36.0	36.0	32.0	36.0
15	34.85608464119646	36.0	36.0	36.0	32.0	36.0
16	34.862422882387285	36.0	36.0	36.0	32.0	36.0
17	34.83043709946325	36.0	36.0	36.0	32.0	36.0
18	34.83394652240895	36.0	36.0	36.0	32.0	36.0
19	34.8267183949688	36.0	36.0	36.0	32.0	36.0
20	34.802918334470604	36.0	36.0	36.0	32.0	36.0
21	34.80201613754195	36.0	36.0	36.0	32.0	36.0
22	34.78898880079914	36.0	36.0	36.0	32.0	36.0
23	34.737824738134265	36.0	36.0	36.0	32.0	36.0
24	34.70469670003447	36.0	36.0	36.0	32.0	36.0
25	34.68341857013218	36.0	36.0	36.0	32.0	36.0
26	34.61683626093012	36.0	36.0	36.0	32.0	36.0
27	34.61608618882472	36.0	36.0	36.0	32.0	36.0
28	34.58581686633416	36.0	36.0	36.0	32.0	36.0
29	34.541371796585366	36.0	36.0	36.0	32.0	36.0
30	34.521171730459436	36.0	36.0	36.0	32.0	36.0
31	34.51994154185983	36.0	36.0	36.0	32.0	36.0
32	34.49040822212687	36.0	36.0	36.0	32.0	36.0
33	34.44503897209345	36.0	36.0	36.0	32.0	36.0
34	34.434983785076646	36.0	36.0	36.0	32.0	36.0
35	34.40342711726098	36.0	36.0	36.0	32.0	36.0
36	34.37353590849296	36.0	36.0	36.0	32.0	36.0
37	34.37657137028413	36.0	36.0	36.0	32.0	36.0
38	33.917167594071174	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	7.0
21	19.0
22	72.0
23	232.0
24	661.0
25	1721.0
26	3790.0
27	7442.0
28	13149.0
29	20878.0
30	32192.0
31	47144.0
32	69216.0
33	111758.0
34	268275.0
35	560668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38749874684954	18.37206520955314	11.875117620262872	26.365318423334454
2	15.953789145830804	20.473311279591847	36.309005629507695	27.26389394506965
3	18.338515543112	23.949811119005577	28.600434039380108	29.111239298502316
4	11.924264367725126	15.775237663106227	35.87163070613488	36.42886726303377
5	14.127835965329579	36.982544320682926	33.12173601835348	15.767883695634014
6	35.030508528230605	35.78374689924492	16.022099447513813	13.163645125010662
7	30.48612448437812	30.3582687239661	20.410228812568203	18.745377979087582
8	28.611087417417707	32.91156604956564	18.831180822353872	19.64616571066278
9	27.16915395323356	13.779752247439506	18.306552243570295	40.74454155575663
10	15.817677396094846	26.68141028741267	31.07213675514305	26.42877556134944
11	38.553778238961634	20.8904561323285	21.411222596445562	19.144543032264306
12	24.619752888840427	23.865721790223713	28.278760240973387	23.23576507996247
13	29.59125798767346	18.897118502032587	25.33324657212626	26.17837693816769
14	23.310095460524927	19.866446715862487	24.8395215014808	31.98393632213179
15	25.169887374870736	27.447450985909548	22.022662201993626	25.359999437226087
16	25.98830660301454	25.89052454971452	23.44931469025864	24.6718541570123
17	24.103254943617088	26.012729242435967	24.79546685613388	25.08854895781306
18	25.2538637946438	24.553649940557005	26.23458527079977	23.957900993999424
19	25.577722594669122	24.689770880670828	25.293345901950715	24.439160622709334
20	25.704434658431406	24.14968379140785	24.949614148136163	25.19626740202458
21	27.183826581218828	24.078281851244785	24.428256878152414	24.309634689383973
22	25.764229386646782	24.166215275090924	25.378641323081467	24.690914015180827
23	24.322779545242707	23.860424789530978	25.862056836747794	25.95473882847852
24	24.69654175430698	25.048978917082298	25.334938411429935	24.919540917180786
25	24.80129227073318	24.49229394762505	25.449889775356283	25.256524006285485
26	24.561321746042772	25.63947440387681	25.819122546765232	23.98008130331518
27	25.485568366478372	24.456219706935485	25.098749234979213	24.95946269160693
28	24.43291734961626	24.648530104886987	26.064170295385956	24.854382250110795
29	24.290201402713976	24.624260479905455	26.029788326662118	25.05574979071845
30	24.196024705774764	25.152652423796894	26.091517590202105	24.559805280226236
31	24.955417754110005	25.01987295378923	24.940293205208473	25.08441608689229
32	24.865110127820024	24.70656616462544	24.780694040927735	25.647629666626802
33	24.266547311699366	24.289058268203977	25.61447876583681	25.829915654259842
34	25.276990285115335	24.70102635892313	25.561630778105283	24.460352577856252
35	25.618171969638347	24.44355729390164	25.859373351248305	24.078897385211707
36	24.244739822585522	25.33529014512532	25.190639662898427	25.22933036939073
37	25.5782501952122	25.07588654477922	24.946184744606164	24.399678515402417
38	24.452723872098964	24.581194717306985	25.63349492579732	25.33258648479674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	18.0
1	30.5
2	43.0
3	43.0
4	221.5
5	400.0
6	400.0
7	594.0
8	788.0
9	934.0
10	1080.0
11	1080.0
12	1472.0
13	1864.0
14	2452.0
15	3040.0
16	3040.0
17	4352.5
18	5665.0
19	5665.0
20	6852.0
21	8039.0
22	8439.0
23	8839.0
24	8839.0
25	10338.5
26	11838.0
27	11838.0
28	15277.5
29	18717.0
30	22542.0
31	26367.0
32	26367.0
33	33136.5
34	39906.0
35	39906.0
36	44053.0
37	48200.0
38	55114.5
39	62029.0
40	62029.0
41	65391.0
42	68753.0
43	78300.0
44	87847.0
45	87847.0
46	88861.0
47	89875.0
48	89875.0
49	96364.0
50	102853.0
51	104639.5
52	106426.0
53	106426.0
54	101209.0
55	95992.0
56	95992.0
57	93998.0
58	92004.0
59	82365.0
60	72726.0
61	72726.0
62	68784.0
63	64842.0
64	53991.0
65	43140.0
66	43140.0
67	36993.0
68	30846.0
69	30846.0
70	24504.0
71	18162.0
72	14174.0
73	10186.0
74	10186.0
75	7633.0
76	5080.0
77	5080.0
78	4992.5
79	4905.0
80	3890.5
81	2876.0
82	2876.0
83	2671.5
84	2467.0
85	2467.0
86	1623.0
87	779.0
88	623.0
89	467.0
90	467.0
91	285.5
92	104.0
93	70.0
94	36.0
95	36.0
96	20.5
97	5.0
98	5.0
99	12.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007914008146152385
2	1.758668476922752E-4
3	0.0
4	4.39667119230688E-4
5	8.79334238461376E-5
6	8.79334238461376E-5
7	4.39667119230688E-4
8	0.0021983355961534405
9	0.0036052703776916425
10	7.914008146152385E-4
11	0.004748404887691431
12	2.6380027153841286E-4
13	2.6380027153841286E-4
14	0.0
15	0.0
16	8.79334238461376E-5
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	1.758668476922752E-4
24	0.0
25	8.79334238461376E-5
26	8.79334238461376E-5
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.79334238461376E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1137224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.50057443973778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14604623344312	40.50211743010333
2	14.203276580589469	14.913603588148435
3	4.189050223743416	6.5978262931032425
4	1.695692406421505	3.5609930164092125
5	0.8348065103242425	2.191391066902781
6	0.4458522878884197	1.4044500737648027
7	0.29181651772664985	1.072437436815713
8	0.18050381558297207	0.7581243205336421
9	0.14190104265860767	0.6704897627857176
>10	0.6819163104949241	6.580329325872277
>50	0.08099768093480099	3.018822532644681
>100	0.09008887564768402	10.240104087680521
>500	0.012990342242040018	4.609525762601437
>1k	0.005061172302093514	3.879785302634232
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3669	0.32262773209147894	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2637	0.23188043868226488	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2074	0.1823739210568894	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1976	0.1737564455199679	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1695	0.14904715341920324	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1602	0.14086934500151244	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1564	0.13752787489535923	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1539	0.13532953929920577	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1526	0.13418640478920602	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1488	0.13084493468305275	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1418	0.12468959501382314	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1405	0.12354646050382334	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1378	0.12117225805997762	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1361	0.1196773898545933	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1313	0.11545658550997867	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1282	0.11273064937074842	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1257	0.11053231377459496	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1226	0.10780637763536471	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1210	0.10639944285382652	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1207	0.1061356425822881	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1168	0.10270623905228873	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1144	0.10059583687998142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.5173369538455047E-4
2	0.0	0.0	0.0	0.0	3.5173369538455047E-4
3	0.0	0.0	0.0	0.0	3.5173369538455047E-4
4	0.0	0.0	0.0	0.0	3.5173369538455047E-4
5	0.0	0.0	0.0	0.0	5.276005430768256E-4
6	0.0	0.0	0.0	0.0	6.155339669229633E-4
7	0.0	0.0	0.0	0.0	7.034673907691009E-4
8	0.0	0.0	0.0	0.0	7.034673907691009E-4
9	0.0	0.0	0.0	0.0	7.034673907691009E-4
10	0.0	0.0	8.793342384613762E-5	8.793342384613762E-5	7.034673907691009E-4
11	0.0	0.0	8.793342384613762E-5	8.793342384613762E-5	7.034673907691009E-4
12	0.0	0.0	8.793342384613762E-5	8.793342384613762E-5	8.793342384613761E-4
13	0.0	0.0	8.793342384613762E-5	1.7586684769227523E-4	0.001143134509999789
14	0.0	0.0	8.793342384613762E-5	1.7586684769227523E-4	0.001143134509999789
15	0.0	0.0	8.793342384613762E-5	1.7586684769227523E-4	0.001582801629230477
16	0.0	0.0	8.793342384613762E-5	2.638002715384128E-4	0.0016707350530766147
17	0.0	0.0	8.793342384613762E-5	3.5173369538455047E-4	0.0018466019007688898
18	0.0	0.0	8.793342384613762E-5	3.5173369538455047E-4	0.002022468748461165
19	0.0	0.0	8.793342384613762E-5	3.5173369538455047E-4	0.0021104021723073025
20	0.0	0.0	8.793342384613762E-5	3.5173369538455047E-4	0.0023742024438457156
21	0.0	0.0	8.793342384613762E-5	4.3966711923068807E-4	0.0023742024438457156
22	0.0	0.0	8.793342384613762E-5	4.3966711923068807E-4	0.002462135867691853
23	0.0	0.0	8.793342384613762E-5	8.793342384613761E-4	0.002462135867691853
24	0.0	0.0	8.793342384613762E-5	0.0021983355961534405	0.0025500692915379907
25	0.0	0.0	8.793342384613762E-5	0.002725936139230266	0.0025500692915379907
26	0.0	0.0	8.793342384613762E-5	0.0029018029869225413	0.002725936139230266
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	285	0.0	20.209665	29
ACGAACG	105	2.8012437E-10	19.80868	15
TTACGCA	50	7.189425E-4	19.202559	4
TCTAGCG	300	0.0	19.199183	28
TCGTTTA	295	0.0	18.982243	30
CGAACGA	110	5.2023097E-10	18.908285	16
ATCGTTT	310	0.0	18.579855	29
CATCGTT	315	0.0	18.284935	28
TAGCGGC	310	0.0	18.063747	30
CGGCATC	325	0.0	17.722322	25
GCATCGT	340	0.0	16.940454	27
GGCATCG	345	0.0	16.69494	26
CGTTTAT	350	0.0	16.456442	31
CGCATCG	360	0.0	16.443745	13
TAGAACA	295	0.0	16.273355	4
CCAGTCG	365	0.0	16.218489	20
CAAGACG	420	0.0	16.002132	4
CAGTCGG	370	0.0	15.999319	21
TAACGAA	140	7.421477E-10	15.999318	13
TGTGTCG	60	0.0024425793	15.999318	10
>>END_MODULE