FastQCFastQC Report
Thu 2 Feb 2017
SRR4062983_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062983_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762403
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT32260.42313579563564147No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21620.28357705832742003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT12330.16172549163631308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9270.12158923823752006No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9080.11909711792844467No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG8020.10519370988833988No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA7750.10165227576491698No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAA601.1302485E-418.6823311
CGAGCCG2000.018.40094215
CGTTAGG450.00884500117.7786915
TAGTACT450.00885838217.7740274
CCTATTC2000.017.5962853
AAATCGC1101.0608346E-817.4554414
ATCTAAC652.0858199E-417.2452281
GTCCTAT2250.017.0809881
CAAATCG1051.1868178E-716.76276413
AGGCCCG2500.016.64085210
GTTATAT908.505202E-616.0134281
CGTCTTA600.002440058616.00081815
TAGACTG1102.0228981E-715.9955735
CCGAGCC2450.015.67427314
CTAGCGG1451.2187229E-915.44805429
TATTCCA2700.015.4031445
GAGCCGC2500.015.36078616
AATCGCT1153.3385004E-715.30513215
GTTCAAA2850.015.1706151
GTATTAG1807.2759576E-1215.1237931
Produced by FastQC (version 0.11.5)