##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062983_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	762403
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178157745968996	32.0	32.0	32.0	32.0	32.0
2	30.767883914412717	32.0	32.0	32.0	32.0	32.0
3	30.849963864255518	32.0	32.0	32.0	32.0	32.0
4	30.91439697902553	32.0	32.0	32.0	32.0	32.0
5	30.81581919273665	32.0	32.0	32.0	32.0	32.0
6	34.473626153097506	36.0	36.0	36.0	32.0	36.0
7	34.378189750040335	36.0	36.0	36.0	32.0	36.0
8	34.35130633011675	36.0	36.0	36.0	32.0	36.0
9	34.49805942526459	36.0	36.0	36.0	32.0	36.0
10	34.20253724080309	36.0	36.0	36.0	32.0	36.0
11	34.52084002817408	36.0	36.0	36.0	32.0	36.0
12	34.320153514611036	36.0	36.0	36.0	32.0	36.0
13	34.41171139148193	36.0	36.0	36.0	32.0	36.0
14	34.31431670651873	36.0	36.0	36.0	32.0	36.0
15	34.25408740521745	36.0	36.0	36.0	32.0	36.0
16	34.26801704610291	36.0	36.0	36.0	32.0	36.0
17	34.1994365184817	36.0	36.0	36.0	32.0	36.0
18	34.216977110530785	36.0	36.0	36.0	32.0	36.0
19	34.2113108159333	36.0	36.0	36.0	32.0	36.0
20	34.16933170514807	36.0	36.0	36.0	32.0	36.0
21	34.14486826520882	36.0	36.0	36.0	32.0	36.0
22	34.13705743550327	36.0	36.0	36.0	32.0	36.0
23	34.081725806430455	36.0	36.0	36.0	32.0	36.0
24	34.08224521676856	36.0	36.0	36.0	32.0	36.0
25	34.05972169574359	36.0	36.0	36.0	32.0	36.0
26	34.01103878132694	36.0	36.0	36.0	32.0	36.0
27	34.028579373376026	36.0	36.0	36.0	32.0	36.0
28	34.01592727205953	36.0	36.0	36.0	32.0	36.0
29	33.97472203021237	36.0	36.0	36.0	32.0	36.0
30	33.94961850884637	36.0	36.0	36.0	32.0	36.0
31	33.95581995348917	36.0	36.0	36.0	32.0	36.0
32	33.90243086661516	36.0	36.0	36.0	32.0	36.0
33	33.85312623376351	36.0	36.0	36.0	32.0	36.0
34	33.8616715831391	36.0	36.0	36.0	32.0	36.0
35	33.817764358219996	36.0	36.0	36.0	32.0	36.0
36	33.7620064454101	36.0	36.0	36.0	32.0	36.0
37	33.77863282279844	36.0	36.0	36.0	32.0	36.0
38	33.15920582683961	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	4.0
10	3.0
11	7.0
12	7.0
13	7.0
14	116.0
15	255.0
16	307.0
17	389.0
18	490.0
19	639.0
20	806.0
21	1037.0
22	1697.0
23	2401.0
24	3494.0
25	5312.0
26	7627.0
27	11074.0
28	15385.0
29	20767.0
30	27791.0
31	37549.0
32	51081.0
33	76777.0
34	167683.0
35	329694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.937885751805645	17.28719632304662	11.200000000000001	25.574917925147734
2	16.537006853072334	20.291760827513077	37.92103349895143	25.250198820463154
3	18.68091793713385	25.169548666042257	28.714132422427387	27.43540097439651
4	12.254109165889496	15.882085204117102	36.721009573953175	35.14279605604022
5	14.126780718334004	37.21693120305141	33.594699915923734	15.061588162690862
6	32.732206893476146	36.42632102224286	17.47752498025976	13.363947104021237
7	29.416725799872246	31.0394896137607	21.51526161360855	18.028522972758502
8	28.005377754459605	32.62749213011543	19.89756033578174	19.46956977964323
9	27.988420791683115	14.00247376380671	18.692131830886897	39.31697361362328
10	15.74752852508988	26.919237826912155	31.993483727024824	25.339749920973137
11	36.897174539506736	21.576780357534016	22.469762811373197	19.056282291586054
12	24.783342668147995	24.420268659026547	28.979418373276282	21.816970299549173
13	29.767581586511156	19.946109677563236	25.224422758297706	25.061885977627906
14	23.69590597247911	19.993309983865252	25.868455918040745	30.442328125614893
15	25.03676745519356	27.12552625675163	23.14335830852651	24.6943479795283
16	25.184242630950664	25.879104301399693	24.350329918274717	24.586323149374927
17	23.590732577665825	25.8485516372796	25.64953295549958	24.911182829554996
18	24.3576491822718	24.98780097487263	26.912361154106478	23.74218868874909
19	25.23506571870819	25.084476742660755	26.231077996694392	23.44937954193667
20	25.593490688053055	24.21617778617283	25.709709662440282	24.48062186333383
21	26.989369739962644	24.221750582408127	24.943464436375848	23.845415241253384
22	25.704500169869704	24.58414715569903	25.50747754637312	24.203875128058147
23	24.15485742994804	24.479127723404787	26.04590943256634	25.320105414080835
24	24.255594356973628	25.4826928475664	25.879263163694468	24.382449631765503
25	24.542252366303057	24.86143270384971	25.888503342013603	24.70781158783363
26	24.248713363830404	25.63498758296065	26.252494230963492	23.863804822245456
27	24.842981797956796	25.22785348116044	25.465807548417224	24.463357172465543
28	23.939859802296052	25.21197557034021	26.32274854660314	24.525416080760593
29	23.935067064572195	25.449513002984293	26.269242121142554	24.346177811300954
30	23.946924523797655	25.532997720314903	26.48304139897637	24.037036356911067
31	24.509546720954514	25.439296076898454	25.252637875218404	24.798519326928627
32	24.10863847732982	25.56113045930648	25.185447533596992	25.144783529766705
33	23.88651168647863	25.051748970923505	25.89337481996081	25.16836452263706
34	24.716713323590913	25.135182644148905	25.99980060396011	24.148303428300068
35	25.35292381358434	25.07835098881637	25.7485815486537	23.820143648945592
36	24.101127133052074	25.904513779568877	25.521587641416367	24.472771445962678
37	24.94015019840619	25.818778080215132	25.25012297904437	23.990948742334307
38	23.999937036959302	25.781922261632424	25.72473083300103	24.493409868407245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	600.0
1	451.5
2	303.0
3	303.0
4	581.0
5	859.0
6	859.0
7	1018.5
8	1178.0
9	1128.5
10	1079.0
11	1079.0
12	1427.0
13	1775.0
14	2113.5
15	2452.0
16	2452.0
17	3679.5
18	4907.0
19	4907.0
20	5713.5
21	6520.0
22	6613.0
23	6706.0
24	6706.0
25	7575.0
26	8444.0
27	8444.0
28	10749.0
29	13054.0
30	16208.5
31	19363.0
32	19363.0
33	23474.0
34	27585.0
35	27585.0
36	30843.5
37	34102.0
38	38768.5
39	43435.0
40	43435.0
41	46659.5
42	49884.0
43	55459.5
44	61035.0
45	61035.0
46	63125.5
47	65216.0
48	65216.0
49	67643.5
50	70071.0
51	69511.5
52	68952.0
53	68952.0
54	65564.5
55	62177.0
56	62177.0
57	60076.5
58	57976.0
59	51793.5
60	45611.0
61	45611.0
62	42604.0
63	39597.0
64	32870.0
65	26143.0
66	26143.0
67	21610.0
68	17077.0
69	17077.0
70	13648.5
71	10220.0
72	7986.5
73	5753.0
74	5753.0
75	4383.0
76	3013.0
77	3013.0
78	2837.0
79	2661.0
80	2095.0
81	1529.0
82	1529.0
83	1491.5
84	1454.0
85	1454.0
86	986.0
87	518.0
88	439.5
89	361.0
90	361.0
91	267.0
92	173.0
93	158.0
94	143.0
95	143.0
96	129.0
97	115.0
98	115.0
99	238.5
100	362.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11844129679447746
2	0.05364616875851748
3	0.011148959277442508
4	0.002885612989455708
5	0.0
6	1.3116422679344125E-4
7	0.0
8	3.934926803803238E-4
9	2.623284535868825E-4
10	5.24656907173765E-4
11	0.0018362991751081775
12	0.0031479414430425905
13	0.014952721854452304
14	0.009574988555921211
15	0.02360956082281943
16	0.012198273091790038
17	0.021379768967330928
18	0.006164718659291739
19	0.0082633462879868
20	0.006427047112878621
21	0.006689375566465505
22	0.0070828682468458285
23	0.009968481236301537
24	0.015346214534832626
25	0.018100663297494896
26	0.018887648658255544
27	0.009312660102334329
28	0.006689375566465505
29	0.01023080968988842
30	0.0022297918554885013
31	0.006164718659291739
32	0.0076075251540195935
33	0.009050331648747448
34	0.013247586906137568
35	0.01678902102956048
36	0.015083886081245746
37	0.01023080968988842
38	0.006689375566465505
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	762403.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.51736995163134
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.54632410941942	50.98061712544209
2	12.388392923709668	15.489794870354585
3	3.351392236170329	6.285606848450566
4	1.1143646999380805	2.7866860082827056
5	0.4719484264577643	1.4752487187475165
6	0.24129424888704817	0.905104909492356
7	0.15172004273581471	0.6639596628554573
8	0.09280234545766869	0.4641406850684935
9	0.06880013660922808	0.38710832338096984
>10	0.42436040256097196	5.426943985565194
>50	0.06913647548609633	3.111603331894263
>100	0.07314056111934615	8.82634988729004
>500	0.00569105230366875	2.324360424978436
>1k	6.323391448520833E-4	0.8724752181973029
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3226	0.42313579563564147	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2162	0.28357705832742003	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1233	0.16172549163631308	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	927	0.12158923823752006	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	908	0.11909711792844467	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	802	0.10519370988833988	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	775	0.10165227576491698	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.3116422679344125E-4	0.0
4	0.0	1.3116422679344125E-4	0.0	1.3116422679344125E-4	0.0
5	0.0	1.3116422679344125E-4	0.0	1.3116422679344125E-4	0.0
6	0.0	1.3116422679344125E-4	0.0	1.3116422679344125E-4	0.0
7	0.0	1.3116422679344125E-4	0.0	1.3116422679344125E-4	0.0
8	0.0	2.623284535868825E-4	0.0	2.623284535868825E-4	0.0
9	0.0	2.623284535868825E-4	0.0	3.934926803803238E-4	0.0
10	0.0	2.623284535868825E-4	0.0	6.558211339672064E-4	0.0
11	0.0	2.623284535868825E-4	0.0	6.558211339672064E-4	0.0
12	0.0	2.623284535868825E-4	0.0	6.558211339672064E-4	3.934926803803238E-4
13	0.0	2.623284535868825E-4	0.0	6.558211339672064E-4	3.934926803803238E-4
14	0.0	3.934926803803238E-4	0.0	6.558211339672064E-4	3.934926803803238E-4
15	0.0	3.934926803803238E-4	0.0	9.181495875540889E-4	3.934926803803238E-4
16	0.0	3.934926803803238E-4	0.0	9.181495875540889E-4	3.934926803803238E-4
17	0.0	3.934926803803238E-4	0.0	9.181495875540889E-4	3.934926803803238E-4
18	0.0	3.934926803803238E-4	0.0	9.181495875540889E-4	5.24656907173765E-4
19	0.0	3.934926803803238E-4	0.0	9.181495875540889E-4	5.24656907173765E-4
20	0.0	3.934926803803238E-4	0.0	0.00104931381434753	7.869853607606476E-4
21	0.0	3.934926803803238E-4	0.0	0.0011804780411409713	9.181495875540889E-4
22	0.0	3.934926803803238E-4	0.0	0.0011804780411409713	0.00104931381434753
23	0.0	3.934926803803238E-4	0.0	0.0026232845358688255	0.0011804780411409713
24	0.0	3.934926803803238E-4	0.0	0.004721912164563885	0.0013116422679344127
25	0.0	5.24656907173765E-4	0.0	0.0055088975253245335	0.0013116422679344127
26	0.0	5.24656907173765E-4	0.0	0.00721403247363927	0.001442806494727854
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAA	60	1.1302485E-4	18.682331	1
CGAGCCG	200	0.0	18.400942	15
CGTTAGG	45	0.008845001	17.77869	15
TAGTACT	45	0.008858382	17.774027	4
CCTATTC	200	0.0	17.596285	3
AAATCGC	110	1.0608346E-8	17.45544	14
ATCTAAC	65	2.0858199E-4	17.245228	1
GTCCTAT	225	0.0	17.080988	1
CAAATCG	105	1.1868178E-7	16.762764	13
AGGCCCG	250	0.0	16.640852	10
GTTATAT	90	8.505202E-6	16.013428	1
CGTCTTA	60	0.0024400586	16.000818	15
TAGACTG	110	2.0228981E-7	15.995573	5
CCGAGCC	245	0.0	15.674273	14
CTAGCGG	145	1.2187229E-9	15.448054	29
TATTCCA	270	0.0	15.403144	5
GAGCCGC	250	0.0	15.360786	16
AATCGCT	115	3.3385004E-7	15.305132	15
GTTCAAA	285	0.0	15.170615	1
GTATTAG	180	7.2759576E-12	15.123793	1
>>END_MODULE