FastQCFastQC Report
Thu 2 Feb 2017
SRR4062983_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062983_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences762403
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT39210.5142949332570832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT27830.36503004316614707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17910.23491513018705332No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10960.14375599256561164No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG9150.12001526751599875No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA8190.10742350174382839No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA8010.10506254566154645No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC7780.10204576844529731No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC7660.10047179772377601No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACTA200.003753821831.998115
GGTCGTA659.4318275E-619.69113729
GTCGTAA701.7868892E-518.28462830
GTATTAG1700.017.8824631
TATTACC550.001364018517.4546532
TACCGTC1950.017.2342667
GTATTAC753.2312124E-517.0667721
CGTAAAC753.2330317E-517.06565332
GAGGTCG854.982312E-616.94016827
ACCGTCG1900.016.8455248
CGTCGTA1950.016.4125110
GCTCGTA1950.016.40928326
TGTACCG1102.0119114E-716.0032465
ATACCGT2100.016.0032466
CCGTCGT2000.016.0021979
GTAGGGC703.6941847E-416.00013
ATATACT703.6941847E-416.00014
TCGAGGT805.6197332E-515.9990525
AGGTCGT805.6197332E-515.9990528
CTCGTAG2100.015.99904927
Produced by FastQC (version 0.11.5)