FastQCFastQC Report
Thu 2 Feb 2017
SRR4062982_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062982_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798915
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28020.3507256716922326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19860.24858714631719267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT10720.13418198431622888No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10410.13030172171006926No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.11340380390905166No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8700.10889769249544695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC507.1350747E-419.2232061
TACCGTC1750.019.1991377
TTATCCG450.0088502717.77697827
CTATACT1201.6643753E-917.327134
ATACCGT1900.016.8371326
CGTTTAT1651.8189894E-1216.48617231
ACCGTCG1850.016.4316948
TCTAGCG1703.6379788E-1216.00128428
CGTCTTA1001.3128993E-616.00028215
GTTATTC1203.1146556E-815.9962753
TAAGGGT908.603425E-615.9942734
TAGGACA805.634644E-515.9942724
CATAGGG1357.888957E-915.4086432
GTATTAT1153.295554E-715.3228451
CGAGCCG2200.015.27299815
CGTTATT1052.1809792E-615.2393182
GTCTTAT1052.1824017E-615.23836316
CCTATTC1901.8189894E-1215.1543663
AATGGAC859.445175E-515.0553193
CCTATAC859.445175E-515.0553193
Produced by FastQC (version 0.11.5)