##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062982_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	798915
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.171717892391555	32.0	32.0	32.0	32.0	32.0
2	30.755913958305953	32.0	32.0	32.0	32.0	32.0
3	30.80605320966561	32.0	32.0	32.0	32.0	32.0
4	30.878371291063505	32.0	32.0	32.0	32.0	32.0
5	30.790855097225613	32.0	32.0	32.0	32.0	32.0
6	34.44128349073431	36.0	36.0	36.0	32.0	36.0
7	34.357521138043474	36.0	36.0	36.0	32.0	36.0
8	34.3284717397971	36.0	36.0	36.0	32.0	36.0
9	34.47334572513972	36.0	36.0	36.0	32.0	36.0
10	34.170588861142924	36.0	36.0	36.0	32.0	36.0
11	34.487577527021024	36.0	36.0	36.0	32.0	36.0
12	34.27279122309633	36.0	36.0	36.0	32.0	36.0
13	34.37321867783181	36.0	36.0	36.0	32.0	36.0
14	34.27470256535426	36.0	36.0	36.0	32.0	36.0
15	34.21764267788188	36.0	36.0	36.0	32.0	36.0
16	34.23322756488488	36.0	36.0	36.0	32.0	36.0
17	34.15801430690374	36.0	36.0	36.0	32.0	36.0
18	34.18650669971149	36.0	36.0	36.0	32.0	36.0
19	34.189503263801534	36.0	36.0	36.0	32.0	36.0
20	34.14255959645269	36.0	36.0	36.0	32.0	36.0
21	34.128853507569644	36.0	36.0	36.0	32.0	36.0
22	34.11137229868009	36.0	36.0	36.0	32.0	36.0
23	34.05467540351602	36.0	36.0	36.0	32.0	36.0
24	34.05537259908751	36.0	36.0	36.0	32.0	36.0
25	34.01694923740323	36.0	36.0	36.0	32.0	36.0
26	33.97399723374827	36.0	36.0	36.0	32.0	36.0
27	33.976699648898816	36.0	36.0	36.0	32.0	36.0
28	33.96033870937459	36.0	36.0	36.0	32.0	36.0
29	33.926323826689945	36.0	36.0	36.0	32.0	36.0
30	33.9041024389328	36.0	36.0	36.0	32.0	36.0
31	33.91134225793733	36.0	36.0	36.0	32.0	36.0
32	33.862131766207916	36.0	36.0	36.0	32.0	36.0
33	33.820302535313516	36.0	36.0	36.0	32.0	36.0
34	33.831945826527225	36.0	36.0	36.0	32.0	36.0
35	33.7782279716866	36.0	36.0	36.0	32.0	36.0
36	33.73248217895521	36.0	36.0	36.0	27.0	36.0
37	33.73126177378069	36.0	36.0	36.0	27.0	36.0
38	33.13596440171983	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	3.0
10	8.0
11	9.0
12	2.0
13	8.0
14	171.0
15	310.0
16	402.0
17	424.0
18	566.0
19	670.0
20	891.0
21	1256.0
22	1729.0
23	2614.0
24	3861.0
25	5707.0
26	8487.0
27	11891.0
28	16711.0
29	22184.0
30	30050.0
31	39716.0
32	53641.0
33	79736.0
34	172016.0
35	345848.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.16627899981954	17.604920695394117	11.648177297427363	25.580623007358987
2	16.26617907662636	20.26800753927751	37.9588846377828	25.506928746313328
3	18.554201181506013	24.70659517708194	28.901830345350778	27.83737329606127
4	12.341545174724335	15.784585304304613	36.6595025328989	35.21436698807216
5	14.195341152320037	36.99465521773416	33.81845264172435	14.991550988221453
6	33.083826713897686	36.12822470616215	17.40158465909802	13.386363920842149
7	29.464461175469232	31.100179618607736	21.35558851692608	18.079770688996952
8	27.851788246750893	32.93721077817179	19.945025165506546	19.26597580957078
9	27.486597414236595	14.431358608509312	19.029356137652034	39.05268783960206
10	15.728304815310862	26.992276977381685	32.19486550424954	25.084552703057916
11	36.54504511834412	21.834800143697763	22.514986249826325	19.105168488131792
12	24.547120497491512	24.393406835501345	29.02997666756792	22.02949599943922
13	29.448688384623665	19.891685632947997	25.522730743903878	25.13689523852446
14	23.575169747831833	20.094337733090313	25.872649067638754	30.4578434514391
15	24.95135906649472	27.061010880044073	23.345895256100462	24.641734797360744
16	25.139580881800654	26.18975488846048	24.257529856538394	24.41313437320047
17	23.546969114603613	26.123068332484067	25.834233724413973	24.495728828498347
18	24.368048027598846	25.095196489058353	27.02478738824896	23.511968095093845
19	25.031169884633876	25.651688431341128	26.138890001602306	23.178251682422687
20	25.18350966617764	24.644747317454197	26.042975810772244	24.12876720559592
21	26.408296874902206	24.654536805803307	25.20908049646056	23.72808582283393
22	25.214086591000413	24.779466714401416	25.641946826661528	24.364499867936644
23	23.99910369363347	24.810911904100227	26.01066053773375	25.179323864532556
24	24.147458399475205	25.703065143692694	25.894354947745835	24.255121509086266
25	24.42191791797807	25.021721113423023	25.985712890698352	24.57064807790055
26	24.11745926422075	25.710959067036416	26.379257460140597	23.792324208602246
27	24.685420432525	25.510863197320127	25.443140337788105	24.360576032366772
28	23.970467842845665	25.271575173560258	26.3309624488958	24.42699453469828
29	23.97572895750189	25.58274036944863	26.258619049288196	24.182911623761285
30	24.036315523972725	25.55693108036556	26.520897198227033	23.88585619743468
31	24.366191364955856	25.53886095328223	25.461625990003267	24.633321691758642
32	23.891831265185644	25.666854016270623	25.586614976535397	24.85469974200833
33	23.734045696030766	25.295301710972357	25.962265473609364	25.008387119387514
34	24.427118737426657	25.49532869394965	26.16169241111499	23.915860157508703
35	25.167318078364705	25.343211755957224	25.872520274719633	23.616949890958438
36	23.963444023661232	25.97502425589183	25.745673061875994	24.31585865857094
37	24.72234601344957	25.844729080812584	25.414227701305396	24.01869720443245
38	23.935953814243028	25.72224704923635	25.936176631479068	24.405622505041553
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	734.0
1	528.0
2	322.0
3	322.0
4	634.5
5	947.0
6	947.0
7	1033.5
8	1120.0
9	1093.5
10	1067.0
11	1067.0
12	1382.0
13	1697.0
14	2159.0
15	2621.0
16	2621.0
17	3755.0
18	4889.0
19	4889.0
20	5800.0
21	6711.0
22	7044.0
23	7377.0
24	7377.0
25	8558.5
26	9740.0
27	9740.0
28	12274.5
29	14809.0
30	17932.0
31	21055.0
32	21055.0
33	25591.5
34	30128.0
35	30128.0
36	33268.5
37	36409.0
38	40648.5
39	44888.0
40	44888.0
41	48416.5
42	51945.0
43	57830.0
44	63715.0
45	63715.0
46	66763.5
47	69812.0
48	69812.0
49	72325.0
50	74838.0
51	73872.5
52	72907.0
53	72907.0
54	69230.0
55	65553.0
56	65553.0
57	62952.5
58	60352.0
59	53721.5
60	47091.0
61	47091.0
62	43636.0
63	40181.0
64	33079.5
65	25978.0
66	25978.0
67	21343.5
68	16709.0
69	16709.0
70	13279.5
71	9850.0
72	7703.0
73	5556.0
74	5556.0
75	4133.5
76	2711.0
77	2711.0
78	2639.0
79	2567.0
80	2031.0
81	1495.0
82	1495.0
83	1472.5
84	1450.0
85	1450.0
86	991.5
87	533.0
88	456.0
89	379.0
90	379.0
91	264.5
92	150.0
93	144.5
94	139.0
95	139.0
96	119.5
97	100.0
98	100.0
99	245.0
100	390.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12254119649775007
2	0.05382299744027838
3	0.013017655194858027
4	0.003504753321692545
5	6.258488074450974E-4
6	6.258488074450974E-4
7	0.0
8	5.006790459560779E-4
9	2.5033952297803894E-4
10	6.258488074450974E-4
11	0.0020027161838243115
12	0.003379583560203526
13	0.01614689923208351
14	0.010389090203588617
15	0.025659801105248996
16	0.01189112734145685
17	0.023531915159935662
18	0.005131960221049799
19	0.008386374019764305
20	0.005882978789983916
21	0.007510185689341169
22	0.00700950664338509
23	0.010138750680610579
24	0.015896559709105473
25	0.019150973507819982
26	0.021278859453133313
27	0.008887053065720383
28	0.007134676404874111
29	0.012892485433369008
30	0.003504753321692545
31	0.006759167120407052
32	0.006508827597429013
33	0.008887053065720383
34	0.012767315671879989
35	0.017148257323995667
36	0.017523766608462728
37	0.009888411157632539
38	0.006759167120407052
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	798915.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.76281633288604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.42242550431942	53.830116118086735
2	10.32439049441595	13.166244296888774
3	2.4756787900646624	4.735687559703437
4	0.9427657215315607	2.4045359018783072
5	0.46560763882115724	1.4844227178671094
6	0.2678324707855217	1.0246651585608173
7	0.18461277454259523	0.8240001305104802
8	0.11908786782812929	0.6074702275040014
9	0.09195336705867482	0.5276885089457463
>10	0.5556858310996302	6.89193091449357
>50	0.07606441121716331	3.4528384729721506
>100	0.06877173275716796	8.350372188214692
>500	0.004335180857105668	1.8329380431947075
>1k	7.882147012919396E-4	0.8670897611795089
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2802	0.3507256716922326	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1986	0.24858714631719267	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1072	0.13418198431622888	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1041	0.13030172171006926	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	906	0.11340380390905166	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	870	0.10889769249544695	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.2516976148901947E-4	0.0
3	0.0	0.0	0.0	1.2516976148901947E-4	0.0
4	0.0	0.0	0.0	1.2516976148901947E-4	0.0
5	0.0	0.0	0.0	1.2516976148901947E-4	0.0
6	0.0	0.0	0.0	1.2516976148901947E-4	0.0
7	0.0	0.0	0.0	1.2516976148901947E-4	0.0
8	0.0	0.0	0.0	1.2516976148901947E-4	0.0
9	0.0	0.0	0.0	1.2516976148901947E-4	0.0
10	0.0	0.0	0.0	1.2516976148901947E-4	0.0
11	0.0	0.0	0.0	1.2516976148901947E-4	0.0
12	0.0	0.0	0.0	1.2516976148901947E-4	1.2516976148901947E-4
13	0.0	0.0	0.0	1.2516976148901947E-4	1.2516976148901947E-4
14	0.0	0.0	0.0	1.2516976148901947E-4	1.2516976148901947E-4
15	0.0	0.0	0.0	1.2516976148901947E-4	2.5033952297803894E-4
16	0.0	0.0	0.0	2.5033952297803894E-4	3.7550928446705847E-4
17	0.0	0.0	0.0	3.7550928446705847E-4	3.7550928446705847E-4
18	0.0	0.0	0.0	3.7550928446705847E-4	5.006790459560779E-4
19	0.0	0.0	0.0	7.510185689341169E-4	5.006790459560779E-4
20	0.0	0.0	0.0	7.510185689341169E-4	5.006790459560779E-4
21	0.0	0.0	0.0	7.510185689341169E-4	5.006790459560779E-4
22	0.0	0.0	0.0	0.0011265278534011752	5.006790459560779E-4
23	0.0	0.0	0.0	0.00237822546829137	5.006790459560779E-4
24	0.0	0.0	0.0	0.003880262606159604	5.006790459560779E-4
25	0.0	0.0	0.0	0.005382299744027838	5.006790459560779E-4
26	0.0	0.0	0.0	0.006884336881896071	6.258488074450975E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	50	7.1350747E-4	19.223206	1
TACCGTC	175	0.0	19.199137	7
TTATCCG	45	0.00885027	17.776978	27
CTATACT	120	1.6643753E-9	17.32713	4
ATACCGT	190	0.0	16.837132	6
CGTTTAT	165	1.8189894E-12	16.486172	31
ACCGTCG	185	0.0	16.431694	8
TCTAGCG	170	3.6379788E-12	16.001284	28
CGTCTTA	100	1.3128993E-6	16.000282	15
GTTATTC	120	3.1146556E-8	15.996275	3
TAAGGGT	90	8.603425E-6	15.994273	4
TAGGACA	80	5.634644E-5	15.994272	4
CATAGGG	135	7.888957E-9	15.408643	2
GTATTAT	115	3.295554E-7	15.322845	1
CGAGCCG	220	0.0	15.272998	15
CGTTATT	105	2.1809792E-6	15.239318	2
GTCTTAT	105	2.1824017E-6	15.238363	16
CCTATTC	190	1.8189894E-12	15.154366	3
AATGGAC	85	9.445175E-5	15.055319	3
CCTATAC	85	9.445175E-5	15.055319	3
>>END_MODULE