FastQCFastQC Report
Thu 2 Feb 2017
SRR4062982_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062982_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences798915
Sequences flagged as poor quality0
Sequence length38
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT35820.44835808565366775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT25880.32393934273358244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT14860.18600226557268293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.14156700024408103No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10820.13543368193111907No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC9760.12216568721328301No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTACG400.00448291319.999539
CGTCTTA701.786511E-518.28528415
ATAACAC450.00884598217.7784753
AATAGCG1850.017.2979745
ACGGTAT1750.016.4567579
CGGGGGC1950.016.40987217
GTTTTCG1950.016.40987228
GTATTAT600.002436318716.0046331
CGACGGT1800.016.0006287
CGTTATT703.693535E-416.0006262
GTAGATC703.693535E-416.0006263
GCGACGC600.002441413715.99962532
AAACGGC1102.0174411E-715.99962522
CTAGCGG1800.015.99962529
CGTATAT2000.015.99962410
CGCGGTC1304.794856E-915.99962410
TCTAGCG1950.015.58937728
GCATTCG1950.015.58937722
AACCGCG1357.892595E-915.40801057
CGCGGTT2400.015.332973510
Produced by FastQC (version 0.11.5)