##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062982_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	798915
Sequences flagged as poor quality	0
Sequence length	38
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.157130608387625	32.0	32.0	32.0	32.0	32.0
2	31.29700906854922	32.0	32.0	32.0	32.0	32.0
3	31.38636275448577	32.0	32.0	32.0	32.0	32.0
4	31.4854070833568	32.0	32.0	32.0	32.0	32.0
5	31.41731348140916	32.0	32.0	32.0	32.0	32.0
6	34.93364125094659	36.0	36.0	36.0	36.0	36.0
7	34.95419412578309	36.0	36.0	36.0	36.0	36.0
8	34.89892291420239	36.0	36.0	36.0	36.0	36.0
9	34.99716114980943	36.0	36.0	36.0	36.0	36.0
10	34.851748934492406	36.0	36.0	36.0	32.0	36.0
11	35.02874773912119	36.0	36.0	36.0	36.0	36.0
12	34.92622243918314	36.0	36.0	36.0	32.0	36.0
13	34.981259583309864	36.0	36.0	36.0	36.0	36.0
14	34.925985868333925	36.0	36.0	36.0	32.0	36.0
15	34.895971411226476	36.0	36.0	36.0	32.0	36.0
16	34.90699886721366	36.0	36.0	36.0	32.0	36.0
17	34.877437524642794	36.0	36.0	36.0	32.0	36.0
18	34.87339078625386	36.0	36.0	36.0	32.0	36.0
19	34.857106200284136	36.0	36.0	36.0	32.0	36.0
20	34.85076009337664	36.0	36.0	36.0	32.0	36.0
21	34.83506005019307	36.0	36.0	36.0	32.0	36.0
22	34.81453596440172	36.0	36.0	36.0	32.0	36.0
23	34.75769262061671	36.0	36.0	36.0	32.0	36.0
24	34.74157200703454	36.0	36.0	36.0	32.0	36.0
25	34.72612480676918	36.0	36.0	36.0	32.0	36.0
26	34.65749172314952	36.0	36.0	36.0	32.0	36.0
27	34.6419969583748	36.0	36.0	36.0	32.0	36.0
28	34.608063436035124	36.0	36.0	36.0	32.0	36.0
29	34.57075533692571	36.0	36.0	36.0	32.0	36.0
30	34.559843037119094	36.0	36.0	36.0	32.0	36.0
31	34.54006871819906	36.0	36.0	36.0	32.0	36.0
32	34.522333414693676	36.0	36.0	36.0	32.0	36.0
33	34.46977713523967	36.0	36.0	36.0	32.0	36.0
34	34.45085647409299	36.0	36.0	36.0	32.0	36.0
35	34.41568877790503	36.0	36.0	36.0	32.0	36.0
36	34.38291683095198	36.0	36.0	36.0	32.0	36.0
37	34.36246784701752	36.0	36.0	36.0	32.0	36.0
38	33.94598924791749	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	3.0
21	14.0
22	55.0
23	182.0
24	532.0
25	1280.0
26	2773.0
27	5427.0
28	9483.0
29	14880.0
30	22581.0
31	32384.0
32	45852.0
33	72707.0
34	175854.0
35	414906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.770730656999184	18.6842240329282	12.335681353581304	26.209363956491316
2	15.353948793050575	21.260083988909958	37.086548631581586	26.299418586457886
3	17.619771815524807	25.402702415150546	29.197223734690176	27.780302034634474
4	11.888999455511536	16.62842730453177	36.61540964933691	34.867163590619775
5	13.640391831911819	37.55648292557146	33.61325499365388	15.18987024886283
6	33.25873215548587	36.554952654537715	16.986663161913345	13.199652028063062
7	29.23413342145318	31.187915565168627	21.383581671072662	18.194369342305535
8	27.7272215198588	33.2262263891149	19.788706830727634	19.257845260298666
9	27.427192365368775	14.182874652954238	18.78165345243251	39.60827952924447
10	15.438246014579828	27.366718737482977	32.151601177601535	25.043434070335657
11	36.6008416720283	21.706225919593034	22.61073544911289	19.082196959265776
12	24.30721319396028	24.523757965530578	29.12276836844154	22.0462604720676
13	29.410586634589748	19.884768429103044	25.552719757971143	25.151925178336064
14	23.324884374432823	20.07422566856299	25.962837097813914	30.638052859190278
15	24.731667323807915	27.221919728631956	23.42214127910979	24.624271668450337
16	25.09716302735585	26.134570010576848	24.241627707578402	24.5266392544889
17	23.433281387882314	26.154096493369135	25.928415413404434	24.48420670534412
18	24.339635630824304	24.96335655232409	27.120156712541384	23.57685110431022
19	24.916543061527197	25.54139051088038	26.3357178172897	23.206348610302722
20	25.220580412184024	24.464304713267367	26.221437825050227	24.093677049498382
21	26.252420476322204	24.584529229090027	25.329917024757385	23.83313326983038
22	25.085052959392375	24.703910558583278	25.808034406707105	24.403002075317243
23	23.676295166056878	24.52206936174527	26.41576742398734	25.385868048210508
24	24.15776396738076	25.4875675134401	26.004643798151246	24.350024721027893
25	24.384727267265312	24.763992119301953	26.247631159298745	24.603649454133986
26	24.030621568779615	25.404236250710337	26.63465654626155	23.93048563424849
27	24.76205138989025	25.171107706480818	25.690939677962028	24.375901225666908
28	23.9418461288122	24.859715989811182	26.65239731385692	24.546040567519697
29	23.945476051895383	25.291927176232765	26.52973094759768	24.23286582427417
30	24.068643097200578	25.249494627087987	26.71247879937165	23.969383476339786
31	24.38106682187717	25.142724820537854	25.747419938291305	24.728788419293668
32	23.968256948486385	25.284041481258956	25.884480827121788	24.863220743132874
33	23.807914484018948	24.927307661015252	26.246972456393987	25.017805398571813
34	24.329246540620716	25.11656434038665	26.55651727655633	23.997671842436304
35	25.145478555290612	24.961353836140265	26.242466344980382	23.65070126358874
36	24.17804146874198	25.46090635424294	25.97247516944857	24.38857700756651
37	24.90202336919447	25.413216675115624	25.713248593404803	23.9715113622851
38	24.063886646263995	25.299812871206573	26.205416095579633	24.4308843869498
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	318.0
1	255.5
2	193.0
3	193.0
4	561.0
5	929.0
6	929.0
7	1071.0
8	1213.0
9	1189.5
10	1166.0
11	1166.0
12	1479.0
13	1792.0
14	2244.5
15	2697.0
16	2697.0
17	4029.5
18	5362.0
19	5362.0
20	6308.0
21	7254.0
22	7512.0
23	7770.0
24	7770.0
25	8759.0
26	9748.0
27	9748.0
28	12170.5
29	14593.0
30	17644.0
31	20695.0
32	20695.0
33	25597.5
34	30500.0
35	30500.0
36	33229.5
37	35959.0
38	40464.0
39	44969.0
40	44969.0
41	48246.0
42	51523.0
43	57578.0
44	63633.0
45	63633.0
46	66676.5
47	69720.0
48	69720.0
49	72413.5
50	75107.0
51	74174.0
52	73241.0
53	73241.0
54	69538.5
55	65836.0
56	65836.0
57	63310.0
58	60784.0
59	54210.0
60	47636.0
61	47636.0
62	44029.0
63	40422.0
64	32963.0
65	25504.0
66	25504.0
67	21089.0
68	16674.0
69	16674.0
70	13308.0
71	9942.0
72	7713.0
73	5484.0
74	5484.0
75	4008.0
76	2532.0
77	2532.0
78	2545.5
79	2559.0
80	1958.5
81	1358.0
82	1358.0
83	1279.5
84	1201.0
85	1201.0
86	755.5
87	310.0
88	267.5
89	225.0
90	225.0
91	134.5
92	44.0
93	31.0
94	18.0
95	18.0
96	10.5
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010764599488055675
2	0.0
3	0.0
4	0.0
5	1.2516976148901947E-4
6	0.0
7	3.7550928446705847E-4
8	0.003129244037225487
9	0.003629923083181565
10	3.7550928446705847E-4
11	0.003629923083181565
12	5.006790459560779E-4
13	2.5033952297803894E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	2.5033952297803894E-4
22	1.2516976148901947E-4
23	2.5033952297803894E-4
24	0.0
25	1.2516976148901947E-4
26	1.2516976148901947E-4
27	3.7550928446705847E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	798915.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.759629455601804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.21902185050115	51.39575953144582
2	11.109456068601855	13.72231780500275
3	2.704723646557195	5.011281905735294
4	0.9995770271081924	2.46934027226136
5	0.487227066785796	1.504548155271526
6	0.29185988475621955	1.081509500129926
7	0.19781104787076004	0.8551715913105715
8	0.14214432681713032	0.7023024762753546
9	0.10514499721660793	0.5844344460487191
>10	0.5801851240911123	7.000752149816498
>50	0.082445550751827	3.616176885300241
>100	0.07387318283848172	8.667892592595894
>500	0.005509878274969985	2.144696884081413
>1k	0.0010203478286981455	1.2438158047246557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3582	0.44835808565366775	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2588	0.32393934273358244	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1486	0.18600226557268293	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1131	0.14156700024408103	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1082	0.13543368193111907	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	976	0.12216568721328301	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	5.006790459560779E-4
13	0.0	0.0	0.0	0.0	8.761883304231364E-4
14	0.0	0.0	0.0	0.0	8.761883304231364E-4
15	0.0	0.0	0.0	0.0	0.0011265278534011752
16	0.0	0.0	0.0	2.5033952297803894E-4	0.0011265278534011752
17	0.0	0.0	0.0	3.7550928446705847E-4	0.0011265278534011752
18	0.0	0.0	0.0	3.7550928446705847E-4	0.0011265278534011752
19	0.0	0.0	0.0	7.510185689341169E-4	0.0011265278534011752
20	0.0	0.0	0.0	7.510185689341169E-4	0.0011265278534011752
21	0.0	0.0	0.0	7.510185689341169E-4	0.0011265278534011752
22	0.0	0.0	0.0	0.001251697614890195	0.0011265278534011752
23	0.0	0.0	0.0	0.002753734752758429	0.0011265278534011752
24	0.0	0.0	0.0	0.004380941652115682	0.0011265278534011752
25	0.0	0.0	0.0	0.0057578090284948966	0.0011265278534011752
26	0.0	0.0	0.0	0.00725984616636313	0.001251697614890195
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTACG	40	0.004482913	19.99953	9
CGTCTTA	70	1.786511E-5	18.285284	15
ATAACAC	45	0.008845982	17.778475	3
AATAGCG	185	0.0	17.297974	5
ACGGTAT	175	0.0	16.456757	9
CGGGGGC	195	0.0	16.409872	17
GTTTTCG	195	0.0	16.409872	28
GTATTAT	60	0.0024363187	16.004633	1
CGACGGT	180	0.0	16.000628	7
CGTTATT	70	3.693535E-4	16.000626	2
GTAGATC	70	3.693535E-4	16.000626	3
GCGACGC	60	0.0024414137	15.999625	32
AAACGGC	110	2.0174411E-7	15.999625	22
CTAGCGG	180	0.0	15.999625	29
CGTATAT	200	0.0	15.999624	10
CGCGGTC	130	4.794856E-9	15.999624	10
TCTAGCG	195	0.0	15.589377	28
GCATTCG	195	0.0	15.589377	22
AACCGCG	135	7.892595E-9	15.4080105	7
CGCGGTT	240	0.0	15.3329735	10
>>END_MODULE