FastQCFastQC Report
Thu 2 Feb 2017
SRR4062981_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062981_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132027
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT29020.25635430957035477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT19370.17110899298338292No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17730.1566217060193794No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17380.15352990697218352No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG16180.1429294530960834No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16140.14257610463354672No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC15700.13868927154564334No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15640.13815924885183833No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15410.13612749519225248No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG15230.13453742711083747No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14240.12579205266305485No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13860.12243524226895647No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13860.12243524226895647No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13250.11704667821527226No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT12720.11236481108666135No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12630.11156977704595385No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG12620.11148143993031968No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12480.11024472031144134No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12100.10688790991734297No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG12100.10688790991734297No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11960.1056511902984646No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC11890.10503283048902544No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG11780.10406112221704958No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11410.10079264893858537No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGG400.004484440719.99922810
TATACTG902.0432708E-819.5513465
TAGCGGC3750.019.19925730
CTAGCGG3750.019.19925729
TCTAGCG3750.019.1984128
ACATCGC652.1009873E-417.2301048
CGAGCCG4600.017.0428215
CTATTCC3900.016.816894
CATCGTT3350.016.71503328
GTGTAGA1701.8189894E-1216.0199051
ACAATAC2100.016.0000883
ATCGTTT3400.015.99938129
CTAGACA1203.1179297E-815.9965544
TAGCACT600.002445387415.9965544
CGGTCCA4900.015.67286310
AGCGGCG4750.015.49413931
GGGTAGA1451.198714E-915.4674951
GTATTGA1255.0582457E-815.3791081
GTATTAT1255.0582457E-815.3791081
CCGGTCC5000.015.35940559
Produced by FastQC (version 0.11.5)