##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062981_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1132027
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266949463219518	32.0	32.0	32.0	32.0	32.0
2	30.905915671622672	32.0	32.0	32.0	32.0	32.0
3	30.96639656121276	32.0	32.0	32.0	32.0	32.0
4	31.024913716722303	32.0	32.0	32.0	32.0	32.0
5	30.945367910836048	32.0	32.0	32.0	32.0	32.0
6	34.59215637082861	36.0	36.0	36.0	32.0	36.0
7	34.53222935495355	36.0	36.0	36.0	32.0	36.0
8	34.50771403862275	36.0	36.0	36.0	32.0	36.0
9	34.644943097646966	36.0	36.0	36.0	32.0	36.0
10	34.37668624511606	36.0	36.0	36.0	32.0	36.0
11	34.62743644807058	36.0	36.0	36.0	32.0	36.0
12	34.45448032599929	36.0	36.0	36.0	32.0	36.0
13	34.53969825808041	36.0	36.0	36.0	32.0	36.0
14	34.43570427207125	36.0	36.0	36.0	32.0	36.0
15	34.39279981837889	36.0	36.0	36.0	32.0	36.0
16	34.397089468714086	36.0	36.0	36.0	32.0	36.0
17	34.32543040051165	36.0	36.0	36.0	32.0	36.0
18	34.35811424992514	36.0	36.0	36.0	32.0	36.0
19	34.33294082208287	36.0	36.0	36.0	32.0	36.0
20	34.322549727170816	36.0	36.0	36.0	32.0	36.0
21	34.29765986146973	36.0	36.0	36.0	32.0	36.0
22	34.280158512120295	36.0	36.0	36.0	32.0	36.0
23	34.21482703151073	36.0	36.0	36.0	32.0	36.0
24	34.200533202829966	36.0	36.0	36.0	32.0	36.0
25	34.172214973671124	36.0	36.0	36.0	32.0	36.0
26	34.12357214094717	36.0	36.0	36.0	32.0	36.0
27	34.12527969739238	36.0	36.0	36.0	32.0	36.0
28	34.11537710672979	36.0	36.0	36.0	32.0	36.0
29	34.08037882488669	36.0	36.0	36.0	32.0	36.0
30	34.054019912952604	36.0	36.0	36.0	32.0	36.0
31	34.07058312213401	36.0	36.0	36.0	32.0	36.0
32	34.02054632972535	36.0	36.0	36.0	32.0	36.0
33	33.973777127224	36.0	36.0	36.0	32.0	36.0
34	33.9887175835912	36.0	36.0	36.0	32.0	36.0
35	33.93415439737745	36.0	36.0	36.0	32.0	36.0
36	33.89947589589294	36.0	36.0	36.0	32.0	36.0
37	33.890885994768674	36.0	36.0	36.0	32.0	36.0
38	33.26976476709478	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	2.0
8	1.0
9	2.0
10	10.0
11	7.0
12	2.0
13	9.0
14	163.0
15	285.0
16	343.0
17	439.0
18	568.0
19	739.0
20	1028.0
21	1468.0
22	2125.0
23	3148.0
24	4462.0
25	6825.0
26	9930.0
27	14553.0
28	20592.0
29	28312.0
30	38481.0
31	52817.0
32	73120.0
33	110859.0
34	247847.0
35	513888.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.83076835990292	17.08979883532517	11.23007280986648	25.84935999490543
2	17.032385228658807	19.48416977496509	36.840760840743165	26.642684155632946
3	18.964546939590473	23.54272628724851	28.319599678767656	29.173127094393365
4	12.562710193437592	14.847794995198734	35.78131666891933	36.80817814244434
5	14.858518900659352	36.326438308728434	33.31484138145481	15.500201409157402
6	34.822698418587564	35.230727916143266	16.44820878672398	13.498364878545194
7	30.699763697798193	30.258342351096484	20.59928005123562	18.442613899869702
8	28.939366902763375	32.01925404276595	19.328511283349616	19.712867771121058
9	27.72401949785428	13.880737300158478	18.425171948955054	39.97007125303219
10	16.109771815187173	25.880703626522827	31.03961852297793	26.969906035312064
11	38.36686666525325	20.82679627705811	21.64472335884552	19.16161369884312
12	25.0653048280078	23.47528575555279	28.167074352539178	23.292335063900232
13	29.540416636848832	18.7380563013695	25.324130887475892	26.397396174305776
14	24.024386688564388	19.49901451416122	24.437133424446234	32.039465372828154
15	25.6070148652374	26.5511129489436	22.198012313477676	25.64385987234133
16	26.467428563351213	25.311950045586585	23.10426958986211	25.116351801200093
17	24.348187187722267	25.421958729639822	24.863558651887914	25.366295430749997
18	25.722945742186603	24.113975154730795	25.872773563870293	24.29030553921231
19	25.996450351298606	24.622264548656602	25.12511672407533	24.256168375969462
20	25.913937648524254	23.734904635281854	24.822565969062786	25.528591747131106
21	27.684624531558928	23.703604235537735	23.90979781721036	24.701973415692972
22	26.19910225795993	23.71895956627098	24.827841487838253	25.25409668793084
23	24.475471344731655	23.592370845668558	25.37915631921309	26.553001490386695
24	24.949244005138326	25.047045373827398	24.698953781564576	25.3047568394697
25	25.26220277721577	24.062488680067112	24.85508277134928	25.820225771367838
26	24.901044685392783	25.19826404105232	25.393612434442105	24.507078839112793
27	25.840538623210236	24.343528667586046	24.54195205857642	25.273980650627298
28	24.79820346532598	24.192791775659323	25.575441469949826	25.43356328906487
29	24.617840612833206	24.59858135226513	25.463746208885237	25.319831826016433
30	24.671796833383834	24.868528803521563	25.73496219512949	24.72471216796511
31	25.30433937594968	24.753259832502916	24.183981766140146	25.75841902540726
32	24.96353627864202	24.58348314894602	24.1819625830538	26.271017989358157
33	24.5873861457864	24.114123664890936	25.069836473986907	26.228653715335753
34	25.584380703995645	24.451312062564824	25.017007073175474	24.947300160264057
35	26.358723261766325	24.144478125693	25.149997393654477	24.3468012188862
36	24.83008252692661	25.18789652195962	24.36677966520979	25.615241285903984
37	26.113709951718988	24.910704425685672	24.35995635714696	24.615629265448376
38	24.837957052987182	24.434760100463535	25.111466642166235	25.615816204383044
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	97.0
1	128.0
2	159.0
3	159.0
4	403.0
5	647.0
6	647.0
7	786.0
8	925.0
9	938.0
10	951.0
11	951.0
12	1236.0
13	1521.0
14	1971.5
15	2422.0
16	2422.0
17	3567.0
18	4712.0
19	4712.0
20	5688.5
21	6665.0
22	7104.0
23	7543.0
24	7543.0
25	9126.5
26	10710.0
27	10710.0
28	13539.5
29	16369.0
30	19666.0
31	22963.0
32	22963.0
33	29855.5
34	36748.0
35	36748.0
36	40447.5
37	44147.0
38	51284.0
39	58421.0
40	58421.0
41	62254.0
42	66087.0
43	76122.0
44	86157.0
45	86157.0
46	88731.5
47	91306.0
48	91306.0
49	97769.5
50	104233.0
51	105821.5
52	107410.0
53	107410.0
54	102712.5
55	98015.0
56	98015.0
57	94842.5
58	91670.0
59	83369.5
60	75069.0
61	75069.0
62	71794.5
63	68520.0
64	57405.5
65	46291.0
66	46291.0
67	38871.0
68	31451.0
69	31451.0
70	25468.0
71	19485.0
72	15331.0
73	11177.0
74	11177.0
75	8399.0
76	5621.0
77	5621.0
78	5494.0
79	5367.0
80	4214.5
81	3062.0
82	3062.0
83	3078.5
84	3095.0
85	3095.0
86	2041.0
87	987.0
88	862.5
89	738.0
90	738.0
91	533.0
92	328.0
93	280.0
94	232.0
95	232.0
96	203.5
97	175.0
98	175.0
99	363.0
100	551.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12464367015981068
2	0.05715411381530652
3	0.012013847726246812
4	0.003003461931561703
5	2.6501134690250325E-4
6	8.833711563416773E-5
7	1.7667423126833547E-4
8	4.4168557817083866E-4
9	4.4168557817083866E-4
10	5.300226938050065E-4
11	0.0016784051970491869
12	0.0024734392377566967
13	0.013603915807661833
14	0.008657037332148438
15	0.022879312949249445
16	0.010158768297929291
17	0.019610839670785238
18	0.005388564053684232
19	0.007420317713270091
20	0.0050352155911475615
21	0.007155306366367587
22	0.0067136207881967475
23	0.009363734257221779
24	0.01245553330441765
25	0.015370658120345186
26	0.01793243447373605
27	0.009187060025953445
28	0.006183598094391741
29	0.009187060025953445
30	0.0026501134690250323
31	0.005918586747489239
32	0.007066969250733419
33	0.00856870021651427
34	0.01280888176695432
35	0.015370658120345186
36	0.013603915807661833
37	0.009893756951026787
38	0.006536946556928412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1132027.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.93777592025076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94766332983154	39.704806082021065
2	13.217817835573747	13.46572486126299
3	4.039970573526781	6.173613473961424
4	1.6911352777600284	3.4457067931748546
5	0.867085898745514	2.208371360695412
6	0.509483116253014	1.5571162086508255
7	0.3228114668401973	1.1510308713675401
8	0.21937563895472445	0.893960571155007
9	0.16528798075785386	0.7577461913538852
>10	0.8000629779558378	7.582505434980337
>50	0.09748090324001729	3.488884639494436
>100	0.1044214299880336	11.529631367336092
>500	0.011834427989453182	4.139773861638043
>1k	0.005569142583272086	3.901128282908127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2902	0.25635430957035477	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1937	0.17110899298338292	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1773	0.1566217060193794	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1738	0.15352990697218352	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1618	0.1429294530960834	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1614	0.14257610463354672	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1570	0.13868927154564334	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1564	0.13815924885183833	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1541	0.13612749519225248	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1523	0.13453742711083747	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1424	0.12579205266305485	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1386	0.12243524226895647	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1386	0.12243524226895647	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1325	0.11704667821527226	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1272	0.11236481108666135	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1263	0.11156977704595385	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1262	0.11148143993031968	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1248	0.11024472031144134	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1210	0.10688790991734297	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1210	0.10688790991734297	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1196	0.1056511902984646	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1189	0.10503283048902544	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1178	0.10406112221704958	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1141	0.10079264893858537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	1.7667423126833547E-4	0.0
12	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	0.0
13	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	0.0
14	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	0.0
15	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	0.0
16	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	0.0
17	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	0.0
18	0.0	1.7667423126833547E-4	0.0	2.650113469025032E-4	0.0
19	0.0	1.7667423126833547E-4	0.0	3.5334846253667093E-4	0.0
20	0.0	1.7667423126833547E-4	0.0	5.300226938050064E-4	0.0
21	0.0	1.7667423126833547E-4	0.0	6.183598094391742E-4	0.0
22	0.0	1.7667423126833547E-4	0.0	9.717082719758451E-4	0.0
23	0.0	1.7667423126833547E-4	0.0	0.001678405197049187	8.833711563416773E-5
24	0.0	1.7667423126833547E-4	0.0	0.0023851021221225287	8.833711563416773E-5
25	0.0	1.7667423126833547E-4	0.0	0.003356810394098374	1.7667423126833547E-4
26	0.0	1.7667423126833547E-4	0.0	0.0037984959722692124	1.7667423126833547E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCGG	40	0.0044844407	19.999228	10
TATACTG	90	2.0432708E-8	19.551346	5
TAGCGGC	375	0.0	19.199257	30
CTAGCGG	375	0.0	19.199257	29
TCTAGCG	375	0.0	19.19841	28
ACATCGC	65	2.1009873E-4	17.230104	8
CGAGCCG	460	0.0	17.04282	15
CTATTCC	390	0.0	16.81689	4
CATCGTT	335	0.0	16.715033	28
GTGTAGA	170	1.8189894E-12	16.019905	1
ACAATAC	210	0.0	16.000088	3
ATCGTTT	340	0.0	15.999381	29
CTAGACA	120	3.1179297E-8	15.996554	4
TAGCACT	60	0.0024453874	15.996554	4
CGGTCCA	490	0.0	15.672863	10
AGCGGCG	475	0.0	15.494139	31
GGGTAGA	145	1.198714E-9	15.467495	1
GTATTGA	125	5.0582457E-8	15.379108	1
GTATTAT	125	5.0582457E-8	15.379108	1
CCGGTCC	500	0.0	15.3594055	9
>>END_MODULE