FastQCFastQC Report
Thu 2 Feb 2017
SRR4062981_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062981_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132027
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT31840.28126537617919006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22650.2000835669113899No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19820.17508416318692044No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17020.1503497708093535No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16850.14884803984357264No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16500.14575624079637678No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16370.14460785829313258No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15780.13939596847071667No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC14760.1303855826760316No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14320.1264987495881282No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14320.1264987495881282No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14260.12596872689432317No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13960.12331861342529817No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13760.12155187111261481No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA13700.1210218484188098No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13270.1172233524465406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13250.11704667821527226No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG13110.1158099585963939No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13100.11572162148075972No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC12010.10609287587663545No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT11960.1056511902984646No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA11920.10529784183592795No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC11880.10494449337339128No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT11760.10388444798578125No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT11750.1037961108701471No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT11670.10308941394507375No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC11410.10079264893858537No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA11370.10043930047604872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGGG659.428997E-619.6934224
AGCGTTC507.197385E-419.1993912
ATACCGT3200.019.0019156
AACGAAT701.7877093E-518.28513331
TACCGTC3550.018.0299857
TCTAGCG3450.017.15887628
TAGGTTG753.230828E-517.0683865
CTACACG753.232053E-517.0676334
ACCCAAT1051.18945536E-716.76137212
ACCGTCG3550.016.6777388
TAGCGGC3550.016.67552830
CAAGACG3850.016.6243174
CGTCTTA1456.91216E-1116.55119915
GTCTTAT1456.91216E-1116.55119916
CTAGCGG3600.016.44392429
ATGTTAG703.6947426E-416.0009061
GTAATAC1001.3135505E-616.0009043
GTCGTAG3600.016.00019811
TATTAGG805.6201417E-516.0001982
ATAACGA1703.6379788E-1215.99949212
Produced by FastQC (version 0.11.5)