##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062981_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1132027
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.166657685726577	32.0	32.0	32.0	32.0	32.0
2	31.23491842509057	32.0	32.0	32.0	32.0	32.0
3	31.34415080205684	32.0	32.0	32.0	32.0	32.0
4	31.455508570025273	32.0	32.0	32.0	32.0	32.0
5	31.354683236353903	32.0	32.0	32.0	32.0	32.0
6	34.886365784561676	36.0	36.0	36.0	36.0	36.0
7	34.91067527541304	36.0	36.0	36.0	36.0	36.0
8	34.84996824280693	36.0	36.0	36.0	32.0	36.0
9	34.97650232724131	36.0	36.0	36.0	36.0	36.0
10	34.797893513140586	36.0	36.0	36.0	32.0	36.0
11	34.993479837495045	36.0	36.0	36.0	36.0	36.0
12	34.86829377744524	36.0	36.0	36.0	32.0	36.0
13	34.94290948890795	36.0	36.0	36.0	36.0	36.0
14	34.877659278444774	36.0	36.0	36.0	32.0	36.0
15	34.849799518916065	36.0	36.0	36.0	32.0	36.0
16	34.857630604217036	36.0	36.0	36.0	32.0	36.0
17	34.823710035184675	36.0	36.0	36.0	32.0	36.0
18	34.8278998645792	36.0	36.0	36.0	32.0	36.0
19	34.80802136344804	36.0	36.0	36.0	32.0	36.0
20	34.80355857236621	36.0	36.0	36.0	32.0	36.0
21	34.793661281930554	36.0	36.0	36.0	32.0	36.0
22	34.773016014635694	36.0	36.0	36.0	32.0	36.0
23	34.710282528596935	36.0	36.0	36.0	32.0	36.0
24	34.6830102108872	36.0	36.0	36.0	32.0	36.0
25	34.65908763660231	36.0	36.0	36.0	32.0	36.0
26	34.59488510433055	36.0	36.0	36.0	32.0	36.0
27	34.58154708324095	36.0	36.0	36.0	32.0	36.0
28	34.53860111110424	36.0	36.0	36.0	32.0	36.0
29	34.502472114181025	36.0	36.0	36.0	32.0	36.0
30	34.48009720616204	36.0	36.0	36.0	32.0	36.0
31	34.47927743772896	36.0	36.0	36.0	32.0	36.0
32	34.45342028061168	36.0	36.0	36.0	32.0	36.0
33	34.4088550891454	36.0	36.0	36.0	32.0	36.0
34	34.39660184783578	36.0	36.0	36.0	32.0	36.0
35	34.357906657703396	36.0	36.0	36.0	32.0	36.0
36	34.3235435197217	36.0	36.0	36.0	32.0	36.0
37	34.31756133025096	36.0	36.0	36.0	32.0	36.0
38	33.85801752078351	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	11.0
21	24.0
22	82.0
23	265.0
24	725.0
25	1781.0
26	4074.0
27	7690.0
28	13355.0
29	21547.0
30	32743.0
31	47687.0
32	68834.0
33	111626.0
34	266106.0
35	555477.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14865121538591	18.377306317886468	11.993338898974773	26.48070356775285
2	16.038133366076956	20.49182572500479	36.01760382040358	27.452437088514674
3	17.974571277893546	24.272654274147172	28.65567694056767	29.097097507391606
4	12.029151299662109	15.790110642432808	35.835692674631744	36.34504538327334
5	14.41334879821771	36.90150499943906	32.957606134836006	15.727540067507222
6	34.98435548300127	35.68486942879404	15.985056880319004	13.345718207885685
7	30.526791882136568	30.45665190252486	20.636878081874734	18.379678133463837
8	28.708015198054394	32.45046100352742	19.07627914454848	19.765244653869697
9	27.536822094051487	13.688552178141816	18.279907102227302	40.494718625579395
10	15.899951944142131	26.154842334383964	31.053271331853967	26.891934389619937
11	38.528990583681036	20.745937837630116	21.528643401641915	19.19642817704694
12	24.8500251319982	23.53821432956751	28.167095544878507	23.444664993555783
13	29.60453205138398	18.762908272424838	25.31549628718775	26.31706338900343
14	23.87840572707188	19.42966024661956	24.436784634995455	32.255149391313104
15	25.679069492158757	26.63540710601426	21.95786849606944	25.727654905757547
16	26.452065987941964	25.356860493363666	23.053996157328683	25.137077361365694
17	24.393322774103446	25.553012428148797	24.748968001646602	25.30469679610115
18	25.774650251274927	24.068595537032245	25.82420737314569	24.332546838547138
19	25.962278284881897	24.72405693503777	25.048695834993335	24.264968945087
20	25.935158790382207	23.66003637722422	24.816545895106742	25.588258937286835
21	27.494156494638816	23.800248404188594	23.968530758127464	24.737064343045123
22	25.98180078743705	23.80676432629257	24.845432131919114	25.366002754351264
23	24.42521247284738	23.43309832716004	25.46061180519546	26.68107739479712
24	25.0131843145084	24.896755996102566	24.746406225293214	25.343653464095823
25	25.237317870790953	24.069235158909777	24.86223814647367	25.8312088238256
26	25.05766215410437	25.037256244340895	25.319140478346323	24.58594112320841
27	25.987500276053975	24.164218988096554	24.541154126454803	25.307126609394672
28	24.766547087657802	24.049073034477093	25.763519774705017	25.42086010316008
29	24.706831197489105	24.39570787622557	25.530839812124622	25.366621114160704
30	24.803737013339788	24.533248765267967	25.921466537458915	24.741547683933334
31	25.352045490081064	24.4557771148568	24.324419823908794	25.867757571153337
32	24.96186045032495	24.41169689415535	24.369825101344755	26.256617554174944
33	24.59163959870215	23.981141792554418	25.207172620441032	26.2200459883024
34	25.57465502147917	24.198804445477005	25.279609055261048	24.946931477782776
35	26.390624958591978	23.98732539064881	25.096839563013955	24.525210087745258
36	24.896932670333836	24.966277306106658	24.540845757212505	25.595944266347004
37	26.247695505495894	24.63483644824726	24.47415123490871	24.64331681134814
38	25.0134493258553	24.120714435256403	25.113093592290642	25.752742646597653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	63.5
2	88.0
3	88.0
4	303.0
5	518.0
6	518.0
7	712.5
8	907.0
9	966.5
10	1026.0
11	1026.0
12	1294.0
13	1562.0
14	2031.5
15	2501.0
16	2501.0
17	3742.0
18	4983.0
19	4983.0
20	5878.5
21	6774.0
22	7349.5
23	7925.0
24	7925.0
25	9252.0
26	10579.0
27	10579.0
28	13335.5
29	16092.0
30	19621.0
31	23150.0
32	23150.0
33	30242.5
34	37335.0
35	37335.0
36	40819.5
37	44304.0
38	51445.5
39	58587.0
40	58587.0
41	62112.5
42	65638.0
43	75541.5
44	85445.0
45	85445.0
46	88726.5
47	92008.0
48	92008.0
49	98321.5
50	104635.0
51	105475.0
52	106315.0
53	106315.0
54	102096.0
55	97877.0
56	97877.0
57	95164.5
58	92452.0
59	83518.0
60	74584.0
61	74584.0
62	71838.0
63	69092.0
64	57897.5
65	46703.0
66	46703.0
67	39419.0
68	32135.0
69	32135.0
70	25859.0
71	19583.0
72	15340.0
73	11097.0
74	11097.0
75	8344.5
76	5592.0
77	5592.0
78	5523.5
79	5455.0
80	4232.5
81	3010.0
82	3010.0
83	2818.0
84	2626.0
85	2626.0
86	1697.5
87	769.0
88	621.5
89	474.0
90	474.0
91	286.0
92	98.0
93	64.0
94	30.0
95	30.0
96	25.5
97	21.0
98	21.0
99	19.5
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007243643482001754
2	0.0
3	0.0
4	1.7667423126833547E-4
5	0.0
6	8.833711563416773E-5
7	4.4168557817083866E-4
8	0.0035334846253667093
9	0.0026501134690250323
10	9.71708271975845E-4
11	0.0049468784755133935
12	3.5334846253667093E-4
13	8.833711563416773E-5
14	0.0
15	0.0
16	1.7667423126833547E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	8.833711563416773E-5
22	0.0
23	0.0
24	0.0
25	8.833711563416773E-5
26	1.7667423126833547E-4
27	1.7667423126833547E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1132027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.02661757425779
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35596698473249	37.43474792873134
2	14.11824503989944	13.843396007816239
3	4.370906149430582	6.428722327233144
4	1.8155783283295959	3.5604665751650137
5	0.951995994724145	2.33365717827829
6	0.5335395810715382	1.5694584601154409
7	0.34227367541903314	1.1746364413353203
8	0.23778777708316398	0.9326344328711313
9	0.1741486367297258	0.7684126752623949
>10	0.8661262323883294	7.83457970809744
>50	0.10305352310757303	3.543453318427757
>100	0.11009704372682723	11.613812868419751
>500	0.014124291088375818	4.71764805236339
>1k	0.006156742269292023	4.244374025883352
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3184	0.28126537617919006	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2265	0.2000835669113899	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1982	0.17508416318692044	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1702	0.1503497708093535	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1685	0.14884803984357264	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1650	0.14575624079637678	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1637	0.14460785829313258	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1578	0.13939596847071667	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1476	0.1303855826760316	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1432	0.1264987495881282	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1432	0.1264987495881282	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1426	0.12596872689432317	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1396	0.12331861342529817	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1376	0.12155187111261481	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1370	0.1210218484188098	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1327	0.1172233524465406	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1325	0.11704667821527226	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1311	0.1158099585963939	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1310	0.11572162148075972	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1201	0.10609287587663545	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1196	0.1056511902984646	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1192	0.10529784183592795	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1188	0.10494449337339128	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1176	0.10388444798578125	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1175	0.1037961108701471	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1167	0.10308941394507375	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1141	0.10079264893858537	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1137	0.10043930047604872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.833711563416773E-5	0.0	0.0	0.0
6	0.0	8.833711563416773E-5	0.0	0.0	8.833711563416773E-5
7	0.0	8.833711563416773E-5	0.0	0.0	8.833711563416773E-5
8	0.0	8.833711563416773E-5	0.0	0.0	8.833711563416773E-5
9	0.0	8.833711563416773E-5	0.0	0.0	8.833711563416773E-5
10	0.0	1.7667423126833547E-4	0.0	0.0	8.833711563416773E-5
11	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	8.833711563416773E-5
12	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	8.833711563416773E-5
13	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	8.833711563416773E-5
14	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	8.833711563416773E-5
15	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	8.833711563416773E-5
16	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	8.833711563416773E-5
17	0.0	1.7667423126833547E-4	0.0	1.7667423126833547E-4	8.833711563416773E-5
18	0.0	1.7667423126833547E-4	0.0	2.650113469025032E-4	8.833711563416773E-5
19	0.0	1.7667423126833547E-4	0.0	3.5334846253667093E-4	8.833711563416773E-5
20	0.0	1.7667423126833547E-4	0.0	4.4168557817083866E-4	8.833711563416773E-5
21	0.0	1.7667423126833547E-4	0.0	5.300226938050064E-4	8.833711563416773E-5
22	0.0	1.7667423126833547E-4	0.0	7.950340407075096E-4	8.833711563416773E-5
23	0.0	1.7667423126833547E-4	0.0	0.0015017309657808515	4.4168557817083866E-4
24	0.0	1.7667423126833547E-4	0.0	0.0022084278908541936	4.4168557817083866E-4
25	0.0	1.7667423126833547E-4	0.0	0.0029151248159275354	4.4168557817083866E-4
26	0.0	1.7667423126833547E-4	0.0	0.003268473278464206	4.4168557817083866E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGGGG	65	9.428997E-6	19.693422	4
AGCGTTC	50	7.197385E-4	19.19939	12
ATACCGT	320	0.0	19.001915	6
AACGAAT	70	1.7877093E-5	18.285133	31
TACCGTC	355	0.0	18.029985	7
TCTAGCG	345	0.0	17.158876	28
TAGGTTG	75	3.230828E-5	17.068386	5
CTACACG	75	3.232053E-5	17.067633	4
ACCCAAT	105	1.18945536E-7	16.761372	12
ACCGTCG	355	0.0	16.677738	8
TAGCGGC	355	0.0	16.675528	30
CAAGACG	385	0.0	16.624317	4
CGTCTTA	145	6.91216E-11	16.551199	15
GTCTTAT	145	6.91216E-11	16.551199	16
CTAGCGG	360	0.0	16.443924	29
ATGTTAG	70	3.6947426E-4	16.000906	1
GTAATAC	100	1.3135505E-6	16.000904	3
GTCGTAG	360	0.0	16.000198	11
TATTAGG	80	5.6201417E-5	16.000198	2
ATAACGA	170	3.6379788E-12	15.999492	12
>>END_MODULE