FastQCFastQC Report
Thu 2 Feb 2017
SRR4062980_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062980_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003995
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT39210.1956591708063144No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT37190.18557930533758818No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC35470.1769964495919401No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC31030.15484070569038347No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28270.14106821623806445No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA28130.140369611700628No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG27510.1372757916062665No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC26570.1325851611406216No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC26120.13033964655600438No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC25810.1287927365088236No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG25180.1256490160903595No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25070.12510011252523087No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG25010.12480071058061522No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG24870.12410210604317876No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC23560.1175651635857375No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC22660.11307413441650305No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG22510.11232562955496396No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC21370.10663699260726699No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG21180.1056888864493175No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT20800.1037926741334185No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC20710.10334357121649505No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG20420.1018964618175195No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA20220.1008984553354674No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG20070.10014995047392833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG5800.020.68923628
TAGGACG4450.020.1304054
CTAGCGG6000.019.99959429
GGTATCA9200.019.8454931
GTATCAA24750.019.4129261
AAGACGG8600.019.3445845
TAGCGGC6400.018.99961530
CAAGACG9000.018.6625634
GACGGAC8750.018.4672767
GTCTAAC1159.295036E-1018.104661
CGCAAGA9050.017.6853792
GCGCAAG9100.017.4236341
AGACGGA9650.017.074396
GTCCTAC5500.016.8892461
AGAGCGA9400.016.85114115
GTAGGAC7050.016.7927283
TAACGAA3450.016.69614613
AGCGAAA9300.016.6882517
ACGGACC9750.016.5731968
ATTTGCC9200.016.5209926
Produced by FastQC (version 0.11.5)