##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062980_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2003995
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.312339601645714	32.0	32.0	32.0	32.0	32.0
2	30.973678078039118	32.0	32.0	32.0	32.0	32.0
3	31.01392468544083	32.0	32.0	32.0	32.0	32.0
4	31.07615587863243	32.0	32.0	32.0	32.0	32.0
5	31.000998505485292	32.0	32.0	32.0	32.0	32.0
6	34.64848016087865	36.0	36.0	36.0	32.0	36.0
7	34.59650098927393	36.0	36.0	36.0	32.0	36.0
8	34.577928088642935	36.0	36.0	36.0	32.0	36.0
9	34.71805867779111	36.0	36.0	36.0	32.0	36.0
10	34.457866411842346	36.0	36.0	36.0	32.0	36.0
11	34.67472124431448	36.0	36.0	36.0	32.0	36.0
12	34.51815199139718	36.0	36.0	36.0	32.0	36.0
13	34.60382685585543	36.0	36.0	36.0	32.0	36.0
14	34.50783559839221	36.0	36.0	36.0	32.0	36.0
15	34.44384841279544	36.0	36.0	36.0	32.0	36.0
16	34.464572017395255	36.0	36.0	36.0	32.0	36.0
17	34.38372650630366	36.0	36.0	36.0	32.0	36.0
18	34.414514507271726	36.0	36.0	36.0	32.0	36.0
19	34.407851815997546	36.0	36.0	36.0	32.0	36.0
20	34.39385178106732	36.0	36.0	36.0	32.0	36.0
21	34.37798497501241	36.0	36.0	36.0	32.0	36.0
22	34.348678015663715	36.0	36.0	36.0	32.0	36.0
23	34.29898777192558	36.0	36.0	36.0	32.0	36.0
24	34.292327575667606	36.0	36.0	36.0	32.0	36.0
25	34.2645166280355	36.0	36.0	36.0	32.0	36.0
26	34.21794565355702	36.0	36.0	36.0	32.0	36.0
27	34.22141123106594	36.0	36.0	36.0	32.0	36.0
28	34.2019351345687	36.0	36.0	36.0	32.0	36.0
29	34.16688714293199	36.0	36.0	36.0	32.0	36.0
30	34.149608656708224	36.0	36.0	36.0	32.0	36.0
31	34.15416755031824	36.0	36.0	36.0	32.0	36.0
32	34.122296712317144	36.0	36.0	36.0	32.0	36.0
33	34.084808095828585	36.0	36.0	36.0	32.0	36.0
34	34.09950773330273	36.0	36.0	36.0	32.0	36.0
35	34.07549619634779	36.0	36.0	36.0	32.0	36.0
36	34.02901304643974	36.0	36.0	36.0	32.0	36.0
37	34.037400791918145	36.0	36.0	36.0	32.0	36.0
38	33.43744670021632	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	5.0
8	1.0
9	5.0
10	17.0
11	21.0
12	9.0
13	8.0
14	127.0
15	362.0
16	511.0
17	543.0
18	759.0
19	1025.0
20	1409.0
21	2076.0
22	3096.0
23	4766.0
24	7503.0
25	11262.0
26	17052.0
27	24539.0
28	35103.0
29	48054.0
30	65500.0
31	89664.0
32	123807.0
33	189008.0
34	433906.0
35	943855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.53041142756653	17.234874221628925	11.319054204203004	25.915660146601542
2	17.157259232187187	18.781117628379874	37.34614685623331	26.715476283199624
3	19.56914152194342	22.245300975612434	28.018676822205375	30.166880680238773
4	12.534001613813524	14.849030589101819	35.80420459802121	36.81276319906345
5	15.01138727100707	35.95897588413526	33.51001401203199	15.51962283282568
6	35.50019810517638	35.23667331009299	16.09452361443643	13.168604970294204
7	31.409890154306925	29.724155723098832	19.93744489127457	18.92850923131967
8	28.489392152048815	33.04770288045637	18.7516092910736	19.711295676421216
9	26.84490783133041	14.230068129116477	18.500101547463586	40.42492249208953
10	16.117394150851506	25.91278173877636	30.897601976862088	27.07222213351005
11	38.6184127795221	20.74371892396839	21.63026533516704	19.007602961342464
12	25.038336765719322	23.373675304436087	28.13581769214916	23.452170237695434
13	29.365042132223913	18.772296340954597	25.27878536222134	26.583876164600145
14	23.447565150562426	19.85138385683345	24.287510854268348	32.413540138335776
15	25.3176573937045	27.499344894796344	21.65845185538272	25.524545856116436
16	26.36398524987037	25.590546662081998	23.462168233123514	24.58329985492412
17	24.397466336524452	26.042920151468795	24.540709858997463	25.01890365300929
18	25.479892568738936	24.43826644829097	25.89383827223704	24.188002710733045
19	25.686613366123428	24.641678522968455	24.858510362765408	24.813197748142706
20	25.865095848957175	24.250579624947978	24.389460058027367	25.49486446806748
21	27.369331397742464	23.9629176986297	24.005485393990718	24.662265509637116
22	26.230805699029368	24.113331836215284	24.82930359058812	24.82655887416723
23	24.603577014403676	23.94832003689002	25.44468143930963	26.003421509396674
24	25.047411719378516	24.814794851985603	25.02734907602045	25.11044435261543
25	25.120105567128853	24.457017029886398	24.992239179019933	25.43063822396482
26	24.64516405952879	25.81306067771943	25.496131467227524	24.04564379552426
27	25.51284904826116	24.451319139677192	24.853503782302276	25.182328029759365
28	24.6231564533241	24.60598977384896	25.566575277386594	25.204278495440352
29	24.502235796702198	24.584780612448597	25.592436219906578	25.320547370942627
30	24.278457855711032	25.138514833550158	25.799463755597106	24.7835635551417
31	25.4296427819314	24.762335356414624	24.370843675799975	25.437178185854
32	25.071199621932127	24.565079723116185	24.3141656182802	26.049555036671485
33	24.498224654225524	24.331095125097626	25.201326456519624	25.969353764157226
34	25.567555994516205	24.604698354014005	25.20073882467786	24.627006826791927
35	25.949817532820134	24.225163302136902	25.57075893570128	24.254260229341682
36	24.266721631456097	25.202387375000434	24.924053267269116	25.60683772627435
37	25.852932839336617	24.699469805249247	24.65320751854484	24.794389836869296
38	24.608120199594087	24.48755331566416	25.132057167466705	25.772269317275047
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	43.5
2	65.0
3	65.0
4	209.5
5	354.0
6	354.0
7	468.5
8	583.0
9	596.5
10	610.0
11	610.0
12	868.0
13	1126.0
14	1855.0
15	2584.0
16	2584.0
17	3836.5
18	5089.0
19	5089.0
20	6881.5
21	8674.0
22	10598.0
23	12522.0
24	12522.0
25	15579.5
26	18637.0
27	18637.0
28	24462.5
29	30288.0
30	37397.5
31	44507.0
32	44507.0
33	57750.0
34	70993.0
35	70993.0
36	78500.0
37	86007.0
38	99099.5
39	112192.0
40	112192.0
41	117388.0
42	122584.0
43	139547.0
44	156510.0
45	156510.0
46	156577.5
47	156645.0
48	156645.0
49	168674.5
50	180704.0
51	186432.0
52	192160.0
53	192160.0
54	183756.5
55	175353.0
56	175353.0
57	168356.0
58	161359.0
59	144971.0
60	128583.0
61	128583.0
62	123001.5
63	117420.0
64	98722.5
65	80025.0
66	80025.0
67	67506.5
68	54988.0
69	54988.0
70	43881.5
71	32775.0
72	25165.5
73	17556.0
74	17556.0
75	13411.5
76	9267.0
77	9267.0
78	9029.5
79	8792.0
80	6921.5
81	5051.0
82	5051.0
83	5076.5
84	5102.0
85	5102.0
86	3391.5
87	1681.0
88	1372.0
89	1063.0
90	1063.0
91	798.5
92	534.0
93	443.5
94	353.0
95	353.0
96	336.0
97	319.0
98	319.0
99	618.5
100	918.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11871287104009741
2	0.055439260077994205
3	0.01137727389539395
4	0.002295414908719832
5	1.4970097230781513E-4
6	4.491029169234455E-4
7	9.98006482052101E-5
8	3.4930226871823534E-4
9	2.9940194461563027E-4
10	5.489035651286555E-4
11	0.001946112640001597
12	0.002195614260514622
13	0.015119798203089329
14	0.008732556717955883
15	0.024950162051302526
16	0.01077847000616269
17	0.02065873417847849
18	0.00543913532718395
19	0.006886244726159497
20	0.00563873662359437
21	0.0063872414851334465
22	0.007235546994877731
23	0.010029965144623615
24	0.013522987831805966
25	0.017016010518988323
26	0.019610827372323784
27	0.009431161255392354
28	0.00563873662359437
29	0.011726576164112186
30	0.002894218797951093
31	0.00603793921641521
32	0.006087839540517816
33	0.007984051856416806
34	0.013572888155908574
35	0.017315412463603953
36	0.014371293341550255
37	0.010129765792828824
38	0.0063872414851334465
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2003995.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.46146783768181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.51131192433324	39.11334573726265
2	13.35786926577473	13.481153806696996
3	4.257578661144758	6.445310060272698
4	1.7688027049431818	3.5702552322677974
5	0.8697052858049404	2.194330265395393
6	0.5038838363592252	1.5256030801421254
7	0.3353867085793431	1.1846873925713737
8	0.22659622782064764	0.9147502609849303
9	0.18264742818705304	0.8294991590786649
>10	0.8138215123105697	7.32554834054934
>50	0.0716061423985435	2.554566222866721
>100	0.0785044731821897	8.80891334849486
>500	0.015222017239822848	5.574180115312978
>1k	0.007063811921747857	6.477856978103517
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3921	0.1956591708063144	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3719	0.18557930533758818	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3547	0.1769964495919401	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	3103	0.15484070569038347	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2827	0.14106821623806445	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2813	0.140369611700628	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2751	0.1372757916062665	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2657	0.1325851611406216	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2612	0.13033964655600438	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	2581	0.1287927365088236	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2518	0.1256490160903595	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2507	0.12510011252523087	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2501	0.12480071058061522	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	2487	0.12410210604317876	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2356	0.1175651635857375	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2266	0.11307413441650305	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2251	0.11232562955496396	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	2137	0.10663699260726699	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2118	0.1056888864493175	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2080	0.1037926741334185	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	2071	0.10334357121649505	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2042	0.1018964618175195	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	2022	0.1008984553354674	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	2007	0.10014995047392833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4970097230781513E-4
2	0.0	0.0	0.0	0.0	1.4970097230781513E-4
3	0.0	0.0	0.0	0.0	1.4970097230781513E-4
4	0.0	0.0	0.0	4.9900324102605044E-5	1.4970097230781513E-4
5	0.0	0.0	0.0	4.9900324102605044E-5	1.4970097230781513E-4
6	0.0	0.0	0.0	4.9900324102605044E-5	1.4970097230781513E-4
7	0.0	0.0	0.0	1.4970097230781513E-4	1.4970097230781513E-4
8	0.0	0.0	0.0	1.4970097230781513E-4	1.4970097230781513E-4
9	0.0	0.0	0.0	1.4970097230781513E-4	1.4970097230781513E-4
10	0.0	0.0	0.0	1.9960129641042018E-4	1.9960129641042018E-4
11	0.0	0.0	0.0	1.9960129641042018E-4	1.9960129641042018E-4
12	0.0	0.0	0.0	1.9960129641042018E-4	2.9940194461563027E-4
13	0.0	0.0	0.0	1.9960129641042018E-4	2.9940194461563027E-4
14	0.0	0.0	0.0	1.9960129641042018E-4	2.9940194461563027E-4
15	0.0	0.0	0.0	2.4950162051302525E-4	2.9940194461563027E-4
16	0.0	0.0	0.0	2.4950162051302525E-4	2.9940194461563027E-4
17	0.0	0.0	0.0	2.4950162051302525E-4	2.9940194461563027E-4
18	0.0	0.0	0.0	2.9940194461563027E-4	2.9940194461563027E-4
19	0.0	0.0	0.0	3.9920259282084035E-4	2.9940194461563027E-4
20	0.0	0.0	0.0	4.491029169234454E-4	3.4930226871823534E-4
21	0.0	0.0	0.0	5.489035651286555E-4	3.4930226871823534E-4
22	0.0	0.0	0.0	6.487042133338656E-4	3.4930226871823534E-4
23	0.0	0.0	0.0	7.984051856416807E-4	3.4930226871823534E-4
24	0.0	0.0	0.0	0.0012475081025651261	3.4930226871823534E-4
25	0.0	0.0	0.0	0.0014471093989755464	3.4930226871823534E-4
26	0.0	0.0	0.0	0.0016467106953859665	3.9920259282084035E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	580	0.0	20.689236	28
TAGGACG	445	0.0	20.130405	4
CTAGCGG	600	0.0	19.999594	29
GGTATCA	920	0.0	19.845493	1
GTATCAA	2475	0.0	19.412926	1
AAGACGG	860	0.0	19.344584	5
TAGCGGC	640	0.0	18.999615	30
CAAGACG	900	0.0	18.662563	4
GACGGAC	875	0.0	18.467276	7
GTCTAAC	115	9.295036E-10	18.10466	1
CGCAAGA	905	0.0	17.685379	2
GCGCAAG	910	0.0	17.423634	1
AGACGGA	965	0.0	17.07439	6
GTCCTAC	550	0.0	16.889246	1
AGAGCGA	940	0.0	16.851141	15
GTAGGAC	705	0.0	16.792728	3
TAACGAA	345	0.0	16.696146	13
AGCGAAA	930	0.0	16.68825	17
ACGGACC	975	0.0	16.573196	8
ATTTGCC	920	0.0	16.52099	26
>>END_MODULE