FastQCFastQC Report
Thu 2 Feb 2017
SRR4062980_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062980_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2003995
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT39060.1949106659447753No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC37670.18797452089451322No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT36610.18268508653963708No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC30240.15089858008627766No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC28970.1445612389252468No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC28660.14301432887806606No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG28190.14066901364524362No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG27950.1394714058667811No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA27670.13807419679190816No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT27250.13597838317959876No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC27100.1352298783180597No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG26750.1334833669744685No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG26390.1316869553067747No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC24050.12001027946676514No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG23880.11916197395702086No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT21700.10828370330265295No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG21700.10828370330265295No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG21360.10658709228316438No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC21010.1048405809395732No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC20640.10299426894777682No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC20110.10034955177033875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22300.020.5924111
CGTAATG400.00448531219.9994682
TCTAGCG6750.019.43603728
CTAGCGG6600.019.39294229
GGTATCA7700.018.4939381
AAGACGG8950.018.058545
TAGCGGC7100.018.0272430
CGCTATA450.008853856517.7773082
GACGGAC9050.016.6212467
GCGCAAG9200.016.3482071
TAGGACG4800.016.3337154
CAAGACG9550.016.251694
CGCAAGA9250.016.086062
AGACGGA9800.016.002376
TCGCGTA4500.016.001979
GTATAGG1501.1459633E-1016.0003741
AGCGGCG8200.015.80406531
ATCGTTT5200.015.69150129
GGCGTTA4650.015.48307331
GCAAGAC10700.015.4018463
Produced by FastQC (version 0.11.5)