##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062980_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2003995
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22676803085836	32.0	32.0	32.0	32.0	32.0
2	31.249204713584614	32.0	32.0	32.0	32.0	32.0
3	31.35190656663315	32.0	32.0	32.0	32.0	32.0
4	31.45106499766716	32.0	32.0	32.0	32.0	32.0
5	31.36579731985359	32.0	32.0	32.0	32.0	32.0
6	34.898455834470646	36.0	36.0	36.0	36.0	36.0
7	34.92682716274242	36.0	36.0	36.0	36.0	36.0
8	34.849248625869826	36.0	36.0	36.0	32.0	36.0
9	34.976983974510915	36.0	36.0	36.0	36.0	36.0
10	34.81771361704994	36.0	36.0	36.0	32.0	36.0
11	34.995561366171074	36.0	36.0	36.0	36.0	36.0
12	34.88649672279622	36.0	36.0	36.0	32.0	36.0
13	34.95342802751504	36.0	36.0	36.0	36.0	36.0
14	34.89524375060816	36.0	36.0	36.0	32.0	36.0
15	34.86339636575939	36.0	36.0	36.0	32.0	36.0
16	34.87089488746229	36.0	36.0	36.0	32.0	36.0
17	34.833806471573034	36.0	36.0	36.0	32.0	36.0
18	34.83480697307129	36.0	36.0	36.0	32.0	36.0
19	34.83522364077755	36.0	36.0	36.0	32.0	36.0
20	34.83209788447576	36.0	36.0	36.0	32.0	36.0
21	34.81509085601511	36.0	36.0	36.0	32.0	36.0
22	34.800376747446975	36.0	36.0	36.0	32.0	36.0
23	34.74365255402334	36.0	36.0	36.0	32.0	36.0
24	34.72006566882652	36.0	36.0	36.0	32.0	36.0
25	34.6961090222281	36.0	36.0	36.0	32.0	36.0
26	34.627401764974465	36.0	36.0	36.0	32.0	36.0
27	34.614669198276445	36.0	36.0	36.0	32.0	36.0
28	34.58462720715371	36.0	36.0	36.0	32.0	36.0
29	34.55494998740017	36.0	36.0	36.0	32.0	36.0
30	34.52745440981639	36.0	36.0	36.0	32.0	36.0
31	34.53238605884745	36.0	36.0	36.0	32.0	36.0
32	34.50420784482995	36.0	36.0	36.0	32.0	36.0
33	34.477940314222344	36.0	36.0	36.0	32.0	36.0
34	34.47923223361336	36.0	36.0	36.0	32.0	36.0
35	34.45734146043279	36.0	36.0	36.0	32.0	36.0
36	34.42305245272568	36.0	36.0	36.0	32.0	36.0
37	34.428938195953585	36.0	36.0	36.0	32.0	36.0
38	33.97842559487424	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	8.0
21	39.0
22	143.0
23	445.0
24	1253.0
25	3194.0
26	6608.0
27	13303.0
28	23026.0
29	36449.0
30	55830.0
31	81484.0
32	119191.0
33	190883.0
34	467170.0
35	1004965.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.91673632545919	18.506249329404163	11.971314717176986	26.605699627959666
2	16.166158099196853	19.800448603913683	36.56830481113975	27.465088485749718
3	18.544507346575216	23.076804083842525	28.299871007662198	30.07881756192006
4	12.07410009620797	15.735741460045748	35.80588146060463	36.38427698314165
5	14.510372785547263	36.47051837480551	33.130987418126004	15.888121421521223
6	35.51329770113967	35.690194526627586	15.735084902991177	13.061422869241559
7	31.152063936574407	29.973063684077633	19.949889869490107	18.924982509857855
8	28.37070367489025	33.33359947423864	18.51392573970122	19.781771111169885
9	26.77181110928136	14.059114431286835	18.311850332599768	40.857224126832044
10	15.883788911432983	26.282776123841927	30.820310043942683	27.013124920782406
11	38.74631278005031	20.67657710213331	21.488900788020846	19.088209329795532
12	24.882871113563997	23.471935225193352	28.014075925566456	23.631117735676195
13	29.399427541482286	18.77042177897273	25.28059050253794	26.54956017700705
14	23.31592643694221	19.85369224973116	24.198563369669085	32.631817943657545
15	25.35315706875516	27.64902108039192	21.448656309022727	25.54916554183019
16	26.401313376530382	25.712439402293917	23.201405193126728	24.684842028048973
17	24.42850406313389	26.189236999094305	24.24901259733682	25.133246340434983
18	25.513886012689653	24.580550350674528	25.718477341510333	24.187086295125486
19	25.697469305063137	24.70684807097822	24.77271649879366	24.82296612516498
20	25.98873749685004	24.266128408504013	24.19861326999319	25.546520824652756
21	27.415192982403614	24.023111880242976	23.813280598565562	24.74841453878785
22	26.230168353797467	24.1778668000004	24.72088239785149	24.87108244835064
23	24.517188209522004	23.94348275190994	25.458111068418503	26.081217970149552
24	25.08359551795289	24.876808574871696	24.88324571668093	25.156350190494486
25	25.053605423167223	24.508444382346266	24.959593212557916	25.478356981928595
26	24.675086190420824	25.837615201250703	25.335268137164153	24.15203047116432
27	25.64345284639218	24.425534420529413	24.690206003713584	25.240806729364824
28	24.625310941394567	24.61622908240789	25.538536772796338	25.21992320340121
29	24.560839722654	24.545869625423215	25.52201976551838	25.371270886404407
30	24.380549851671287	25.014034466153856	25.730902522211878	24.874513159962973
31	25.496271198281434	24.705800164172068	24.376258423798465	25.421670213748037
32	25.162188528414493	24.61223705647968	24.2658789068835	25.959695508222325
33	24.53903328102116	24.458094955326732	25.04187884700311	25.960992916648994
34	25.659944261337976	24.47176764413085	25.21443416774992	24.653853926781256
35	26.002559886626464	24.261337977390163	25.399763971466992	24.336338164516377
36	24.317276240709184	25.177757429534502	24.905601061878897	25.599365267877417
37	25.966881154893102	24.7121874056572	24.484741728397527	24.836189711052175
38	24.735590657661323	24.300210329866093	25.095222293468794	25.86897671900379
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	22.0
2	37.0
3	37.0
4	141.5
5	246.0
6	246.0
7	347.0
8	448.0
9	482.0
10	516.0
11	516.0
12	752.0
13	988.0
14	1703.5
15	2419.0
16	2419.0
17	3733.5
18	5048.0
19	5048.0
20	6604.0
21	8160.0
22	10227.0
23	12294.0
24	12294.0
25	15740.5
26	19187.0
27	19187.0
28	24779.0
29	30371.0
30	37326.0
31	44281.0
32	44281.0
33	58001.0
34	71721.0
35	71721.0
36	79223.5
37	86726.0
38	99661.5
39	112597.0
40	112597.0
41	117377.0
42	122157.0
43	139579.0
44	157001.0
45	157001.0
46	156719.5
47	156438.0
48	156438.0
49	168723.5
50	181009.0
51	185882.5
52	190756.0
53	190756.0
54	183508.0
55	176260.0
56	176260.0
57	169357.5
58	162455.0
59	146106.0
60	129757.0
61	129757.0
62	123661.5
63	117566.0
64	98629.5
65	79693.0
66	79693.0
67	67731.5
68	55770.0
69	55770.0
70	44519.0
71	33268.0
72	25590.5
73	17913.0
74	17913.0
75	13396.0
76	8879.0
77	8879.0
78	8699.0
79	8519.0
80	6773.5
81	5028.0
82	5028.0
83	4609.5
84	4191.0
85	4191.0
86	2705.5
87	1220.0
88	1001.5
89	783.0
90	783.0
91	469.0
92	155.0
93	111.5
94	68.0
95	68.0
96	50.0
97	32.0
98	32.0
99	31.5
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008982058338468909
2	0.0
3	0.0
4	1.4970097230781513E-4
5	4.990032410260505E-5
6	9.98006482052101E-5
7	4.491029169234455E-4
8	0.002195614260514622
9	0.0042415275487214295
10	0.0010479068061547058
11	0.005688636947696975
12	2.9940194461563027E-4
13	3.4930226871823534E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.996012964104202E-4
22	4.990032410260505E-5
23	2.495016205130252E-4
24	0.0
25	0.0
26	9.98006482052101E-5
27	9.98006482052101E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	2003995.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.55117545289817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.69916107931661	36.75283251199101
2	14.425885013258528	14.00787348684098
3	4.5650767845576015	6.64919531769025
4	1.9325501396339113	3.753103236035556
5	0.9579462439107236	2.325470808127716
6	0.5388248952035901	1.5696349215251384
7	0.3636401868095894	1.235861095806193
8	0.2609461752302807	1.0135394829894455
9	0.18535068491700696	0.8099094261348406
>10	0.8921997109980524	7.728284159140113
>50	0.06932530237578818	2.3720125775283307
>100	0.08404717260290906	8.88198245002186
>500	0.016352746145825343	5.565203033505571
>1k	0.008693865039552714	7.335097492662899
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	3906	0.1949106659447753	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	3767	0.18797452089451322	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	3661	0.18268508653963708	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	3024	0.15089858008627766	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2897	0.1445612389252468	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	2866	0.14301432887806606	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	2819	0.14066901364524362	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2795	0.1394714058667811	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2767	0.13807419679190816	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2725	0.13597838317959876	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	2710	0.1352298783180597	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	2675	0.1334833669744685	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGG	2639	0.1316869553067747	No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCC	2405	0.12001027946676514	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2388	0.11916197395702086	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTAT	2170	0.10828370330265295	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	2170	0.10828370330265295	No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACG	2136	0.10658709228316438	No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTC	2101	0.1048405809395732	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	2064	0.10299426894777682	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCC	2011	0.10034955177033875	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	4.9900324102605044E-5
6	0.0	0.0	0.0	0.0	9.980064820521009E-5
7	0.0	0.0	0.0	0.0	9.980064820521009E-5
8	0.0	0.0	0.0	0.0	9.980064820521009E-5
9	0.0	0.0	0.0	4.9900324102605044E-5	9.980064820521009E-5
10	0.0	0.0	0.0	9.980064820521009E-5	9.980064820521009E-5
11	0.0	0.0	0.0	9.980064820521009E-5	9.980064820521009E-5
12	0.0	0.0	0.0	9.980064820521009E-5	1.9960129641042018E-4
13	0.0	0.0	0.0	9.980064820521009E-5	2.4950162051302525E-4
14	0.0	0.0	0.0	9.980064820521009E-5	2.9940194461563027E-4
15	0.0	0.0	0.0	1.4970097230781513E-4	2.9940194461563027E-4
16	0.0	0.0	0.0	1.4970097230781513E-4	2.9940194461563027E-4
17	0.0	0.0	0.0	1.4970097230781513E-4	3.4930226871823534E-4
18	0.0	0.0	0.0	1.9960129641042018E-4	3.4930226871823534E-4
19	0.0	0.0	0.0	2.4950162051302525E-4	4.491029169234454E-4
20	0.0	0.0	0.0	2.4950162051302525E-4	4.491029169234454E-4
21	0.0	0.0	0.0	2.9940194461563027E-4	4.491029169234454E-4
22	0.0	0.0	0.0	3.9920259282084035E-4	4.491029169234454E-4
23	0.0	0.0	0.0	5.489035651286555E-4	4.491029169234454E-4
24	0.0	0.0	0.0	9.481061579494959E-4	4.491029169234454E-4
25	0.0	0.0	0.0	0.001147707454359916	4.491029169234454E-4
26	0.0	0.0	0.0	0.0013473087507703363	4.990032410260505E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2230	0.0	20.592411	1
CGTAATG	40	0.004485312	19.999468	2
TCTAGCG	675	0.0	19.436037	28
CTAGCGG	660	0.0	19.392942	29
GGTATCA	770	0.0	18.493938	1
AAGACGG	895	0.0	18.05854	5
TAGCGGC	710	0.0	18.02724	30
CGCTATA	45	0.0088538565	17.777308	2
GACGGAC	905	0.0	16.621246	7
GCGCAAG	920	0.0	16.348207	1
TAGGACG	480	0.0	16.333715	4
CAAGACG	955	0.0	16.25169	4
CGCAAGA	925	0.0	16.08606	2
AGACGGA	980	0.0	16.00237	6
TCGCGTA	450	0.0	16.00197	9
GTATAGG	150	1.1459633E-10	16.000374	1
AGCGGCG	820	0.0	15.804065	31
ATCGTTT	520	0.0	15.691501	29
GGCGTTA	465	0.0	15.483073	31
GCAAGAC	1070	0.0	15.401846	3
>>END_MODULE