FastQCFastQC Report
Thu 2 Feb 2017
SRR4062979_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062979_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246676
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT47790.3833393760688422No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT30080.24128161607346257No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG22440.17999865241650598No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA21440.17197732209491481No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20830.16708431059874418No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG19340.1551325284195733No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC19230.1542501820841983No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC19090.15312719583917553No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG18860.15128228986520956No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA18820.15096143665234593No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT18590.14911653067837996No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC18490.14831439764622084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT17910.14366202605969797No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG17330.13900965447317506No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG16700.13395621637057264No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC16630.13339472324806126No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT16520.13251237691268622No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC16300.13074768424193617No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT16190.12986533790656113No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA15460.12400976677179956No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA15150.1215231543721063No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT14920.11967824839814033No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT14820.11887611536598121No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC14560.1167905694823675No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT14430.11574779654056067No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14060.11277990432157192No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG13910.11157670477333324No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13800.11069435843795823No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT13700.1098922254057991No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT13150.10548049372892396No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.10419708087746936No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT12880.10331473454209435No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC12800.10267302811636705No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC12760.1023521749035034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTATA1257.2759576E-1219.2026232
TCTAGCG4450.017.61755828
CCGACGT550.001364951317.4541326
CTAGCGG4500.017.0662629
ACGCTTC3750.017.06557531
ATCGTTT4050.016.9872529
CATCGTT4100.016.38985428
TAGAACA3250.016.2425084
TAGCGGC4750.016.16738730
AAACGCT4100.015.99961929
CGCTTCG4000.015.99897832
TCATAAC600.002443757615.9983363
CGGCATC4250.015.81138925
AACGCTT4100.015.60875830
GTCCTAC1753.6379788E-1215.5530941
GGCATCG4350.015.44790726
GTCCTAT5200.015.3947481
TCGTTTA4650.015.13881830
GCATCGT4450.015.10076327
GTTATTC2650.015.0927693
Produced by FastQC (version 0.11.5)