##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062979_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1246676
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174036397588466	32.0	32.0	32.0	32.0	32.0
2	30.8050832774514	32.0	32.0	32.0	32.0	32.0
3	30.86541731773131	32.0	32.0	32.0	32.0	32.0
4	30.92322784749205	32.0	32.0	32.0	32.0	32.0
5	30.869460870346426	32.0	32.0	32.0	32.0	32.0
6	34.49439870503643	36.0	36.0	36.0	32.0	36.0
7	34.40863223483888	36.0	36.0	36.0	32.0	36.0
8	34.402041909846666	36.0	36.0	36.0	32.0	36.0
9	34.51063708613946	36.0	36.0	36.0	32.0	36.0
10	34.23505626161088	36.0	36.0	36.0	32.0	36.0
11	34.512880652230415	36.0	36.0	36.0	32.0	36.0
12	34.32759995379714	36.0	36.0	36.0	32.0	36.0
13	34.419340710818204	36.0	36.0	36.0	32.0	36.0
14	34.316449502517095	36.0	36.0	36.0	32.0	36.0
15	34.26546111419486	36.0	36.0	36.0	32.0	36.0
16	34.27459500303206	36.0	36.0	36.0	32.0	36.0
17	34.190525846330566	36.0	36.0	36.0	32.0	36.0
18	34.227391078355566	36.0	36.0	36.0	32.0	36.0
19	34.2015664053852	36.0	36.0	36.0	32.0	36.0
20	34.17434201027372	36.0	36.0	36.0	32.0	36.0
21	34.16393754271358	36.0	36.0	36.0	32.0	36.0
22	34.13408375552269	36.0	36.0	36.0	32.0	36.0
23	34.08549214070055	36.0	36.0	36.0	32.0	36.0
24	34.07388687999127	36.0	36.0	36.0	32.0	36.0
25	34.05256698612951	36.0	36.0	36.0	32.0	36.0
26	34.01188119447234	36.0	36.0	36.0	32.0	36.0
27	34.019126862151836	36.0	36.0	36.0	32.0	36.0
28	34.002327790059326	36.0	36.0	36.0	32.0	36.0
29	33.962392794920255	36.0	36.0	36.0	32.0	36.0
30	33.94125658952286	36.0	36.0	36.0	32.0	36.0
31	33.95684203433771	36.0	36.0	36.0	32.0	36.0
32	33.90097106224873	36.0	36.0	36.0	32.0	36.0
33	33.85539466549448	36.0	36.0	36.0	32.0	36.0
34	33.862491938563025	36.0	36.0	36.0	32.0	36.0
35	33.823173783725686	36.0	36.0	36.0	32.0	36.0
36	33.77891047874508	36.0	36.0	36.0	32.0	36.0
37	33.779979722076945	36.0	36.0	36.0	32.0	36.0
38	33.16988215061492	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	5.0
9	4.0
10	12.0
11	17.0
12	9.0
13	7.0
14	224.0
15	436.0
16	504.0
17	581.0
18	794.0
19	1020.0
20	1319.0
21	1926.0
22	2893.0
23	4303.0
24	6107.0
25	9215.0
26	12910.0
27	18353.0
28	25513.0
29	33874.0
30	44629.0
31	59720.0
32	80929.0
33	120019.0
34	265774.0
35	555574.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.869314494625314	17.27200151634894	11.237848326554738	25.62083566247101
2	16.99831693475052	20.17535340758511	37.00688876921138	25.81944088845298
3	18.730706286301064	24.80730353303597	28.336809678143954	28.125180502519015
4	12.540228262299498	15.428641560166719	35.957328362088056	36.07380181544573
5	14.828908623191486	36.51558989406203	32.82673162890349	15.828769853842989
6	33.64068495963654	35.83324242463134	16.976638602615605	13.549434013116521
7	29.929668975740288	30.580439504730982	21.14847803278478	18.34141348674395
8	28.543823581078858	31.698069014309333	19.734588732053176	20.023518672558634
9	28.209871096601347	13.674829746444528	18.60019090858046	39.515108248373664
10	16.522615903043878	25.897613396360054	30.454644263464097	27.125126437131968
11	38.0158858758168	21.043731881325993	22.09230877830203	18.84807346455518
12	25.60545084238906	23.727672859614657	28.055570484657473	22.61130581333881
13	29.716711312854656	18.930144159452276	25.26717514382651	26.08596938386656
14	24.456976912092674	20.017488407913103	24.454490028398606	31.071044651595614
15	25.536845010574826	26.574269309892195	22.426497726171334	25.46238795336164
16	26.21791652560661	25.38695915168221	23.41401701063927	24.981107312071906
17	24.21116563275235	25.30897082468172	24.870025256820465	25.60983828574546
18	25.528199237281747	23.992173875375624	26.12406284644399	24.355564040898642
19	26.07234250784152	24.298996446248506	25.202595923213295	24.42606512269668
20	26.332014801991676	23.415817081652413	24.54586074480041	25.706307371555496
21	28.350103202278877	23.484795612960568	23.817625970554662	24.347475214205897
22	26.68125605662908	23.47172396531918	24.65770018161865	25.189319796433086
23	24.57036596107903	23.28254685421724	25.52509935613056	26.621987828573168
24	25.156863358596738	24.781402155051037	24.95597556934921	25.10575891700302
25	25.253222189684983	23.924071451780478	25.08899381034711	25.73371254818743
26	24.848486307205743	25.23607574320736	25.659367373168955	24.256070576417937
27	25.784509639859998	24.062788046621737	24.648969189189625	25.503733124328644
28	24.489386638258477	24.30063156341023	25.727324723706047	25.482657074625237
29	24.505208437789552	24.45731567906236	25.53654730914094	25.500928574007148
30	24.232829868116124	24.871994590202203	26.023582033759894	24.871593507921776
31	25.37281584862433	24.679326228106344	24.206999231506007	25.740858691763318
32	24.982431933770798	24.6332365351602	24.15504821190778	26.229283319161222
33	24.43742955585594	23.931234482233034	25.248063663456488	26.383272298454543
34	25.692061092953516	24.333129561794923	25.137545788427378	24.83726355682418
35	26.447123723763237	23.931555360154295	25.36973359284617	24.251587323236297
36	24.578690221184626	25.268718862066475	24.508973340714178	25.64361757603472
37	25.885859144986483	25.048494621068084	24.557852598814307	24.507793635131122
38	24.68181198308987	24.665046246159523	25.058760298093198	25.594381472657407
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1052.0
1	744.0
2	436.0
3	436.0
4	847.5
5	1259.0
6	1259.0
7	1453.0
8	1647.0
9	1567.0
10	1487.0
11	1487.0
12	1937.5
13	2388.0
14	2915.0
15	3442.0
16	3442.0
17	5021.5
18	6601.0
19	6601.0
20	7797.5
21	8994.0
22	8824.5
23	8655.0
24	8655.0
25	10127.5
26	11600.0
27	11600.0
28	14922.5
29	18245.0
30	22240.5
31	26236.0
32	26236.0
33	33629.0
34	41022.0
35	41022.0
36	44990.5
37	48959.0
38	56738.5
39	64518.0
40	64518.0
41	67755.5
42	70993.0
43	82236.0
44	93479.0
45	93479.0
46	94335.5
47	95192.0
48	95192.0
49	103548.0
50	111904.0
51	113693.0
52	115482.0
53	115482.0
54	110530.0
55	105578.0
56	105578.0
57	104467.5
58	103357.0
59	92799.5
60	82242.0
61	82242.0
62	80398.0
63	78554.0
64	65328.5
65	52103.0
66	52103.0
67	43746.0
68	35389.0
69	35389.0
70	28267.0
71	21145.0
72	16456.5
73	11768.0
74	11768.0
75	8793.5
76	5819.0
77	5819.0
78	5926.0
79	6033.0
80	4739.5
81	3446.0
82	3446.0
83	3642.5
84	3839.0
85	3839.0
86	2566.0
87	1293.0
88	1094.5
89	896.0
90	896.0
91	677.0
92	458.0
93	377.0
94	296.0
95	296.0
96	278.0
97	260.0
98	260.0
99	434.5
100	609.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12673701908114057
2	0.05895677786369514
3	0.012513275301682234
4	0.0032085321286364704
5	2.4063990964773527E-4
6	3.20853212863647E-4
7	0.0
8	5.614931225113823E-4
9	4.8127981929547053E-4
10	7.219197289432058E-4
11	0.0027272523093409998
12	0.0034491720382842056
13	0.016764580372125554
14	0.010909009237363999
15	0.025668257029091764
16	0.012994555120977703
17	0.02245972490045529
18	0.006577490863704764
19	0.008502610140886647
20	0.006978557379784322
21	0.007620263805511616
22	0.00866303674731847
23	0.012031995482386763
24	0.016764580372125554
25	0.01933140607503473
26	0.020374179016841583
27	0.010668369327716263
28	0.006978557379784322
29	0.011310075753443557
30	0.003930451857579676
31	0.006577490863704764
32	0.0077004771087275286
33	0.009705809689125322
34	0.014598821185295938
35	0.019090766165386998
36	0.01652394046247782
37	0.011711142269523116
38	0.006898344076568411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1246676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.050351937164464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.30063906050485	36.37022272764476
2	13.502095136396331	12.70556656113048
3	4.278409468683043	6.0390201369850205
4	1.7564817448413286	3.3057233706395692
5	0.8697514525476859	2.0461055970114312
6	0.5108886915711459	1.442249564348393
7	0.30887680321548766	1.0172933607560493
8	0.22431711864294768	0.8443359502145092
9	0.1653155838171537	0.7000340759365403
>10	0.8353150838440481	7.319622206165066
>50	0.09828875959405405	3.266728271568172
>100	0.12171693550572255	12.45668867258011
>500	0.019686984639177178	6.39374531684843
>1k	0.008217176197221778	6.092664188171441
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4779	0.3833393760688422	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3008	0.24128161607346257	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2244	0.17999865241650598	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2144	0.17197732209491481	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2083	0.16708431059874418	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1934	0.1551325284195733	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1923	0.1542501820841983	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1909	0.15312719583917553	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1886	0.15128228986520956	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1882	0.15096143665234593	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1859	0.14911653067837996	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1849	0.14831439764622084	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1791	0.14366202605969797	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1733	0.13900965447317506	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1670	0.13395621637057264	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1663	0.13339472324806126	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1652	0.13251237691268622	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1630	0.13074768424193617	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1619	0.12986533790656113	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1546	0.12400976677179956	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1515	0.1215231543721063	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1492	0.11967824839814033	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	1482	0.11887611536598121	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1456	0.1167905694823675	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1443	0.11574779654056067	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1406	0.11277990432157192	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1391	0.11157670477333324	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1380	0.11069435843795823	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1370	0.1098922254057991	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	1315	0.10548049372892396	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1299	0.10419708087746936	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1288	0.10331473454209435	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATC	1280	0.10267302811636705	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	1276	0.1023521749035034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.021330321591176E-5
2	0.0	0.0	0.0	0.0	8.021330321591176E-5
3	0.0	0.0	0.0	0.0	8.021330321591176E-5
4	0.0	0.0	0.0	0.0	8.021330321591176E-5
5	0.0	0.0	0.0	0.0	8.021330321591176E-5
6	0.0	0.0	0.0	0.0	8.021330321591176E-5
7	0.0	0.0	0.0	0.0	8.021330321591176E-5
8	0.0	0.0	0.0	0.0	8.021330321591176E-5
9	0.0	0.0	0.0	8.021330321591176E-5	8.021330321591176E-5
10	0.0	0.0	0.0	1.604266064318235E-4	1.604266064318235E-4
11	0.0	0.0	0.0	1.604266064318235E-4	1.604266064318235E-4
12	0.0	0.0	0.0	1.604266064318235E-4	7.219197289432057E-4
13	0.0	0.0	0.0	1.604266064318235E-4	7.219197289432057E-4
14	0.0	0.0	0.0	1.604266064318235E-4	7.219197289432057E-4
15	0.0	0.0	0.0	1.604266064318235E-4	7.219197289432057E-4
16	0.0	0.0	0.0	1.604266064318235E-4	7.219197289432057E-4
17	0.0	0.0	0.0	1.604266064318235E-4	7.219197289432057E-4
18	0.0	0.0	0.0	3.20853212863647E-4	7.219197289432057E-4
19	0.0	0.0	0.0	3.20853212863647E-4	7.219197289432057E-4
20	0.0	0.0	0.0	4.0106651607955875E-4	8.823463353750293E-4
21	0.0	0.0	0.0	4.8127981929547053E-4	9.625596385909411E-4
22	0.0	0.0	0.0	0.001283412851454588	0.0010427729418068527
23	0.0	0.0	0.0	0.002566825702909176	0.0010427729418068527
24	0.0	0.0	0.0	0.0038502385543637643	0.0010427729418068527
25	0.0	0.0	0.0	0.0042513050704433225	0.0010427729418068527
26	0.0	0.0	0.0	0.0052940780122501755	0.0011229862450227645
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTATA	125	7.2759576E-12	19.202623	2
TCTAGCG	445	0.0	17.617558	28
CCGACGT	55	0.0013649513	17.45413	26
CTAGCGG	450	0.0	17.06626	29
ACGCTTC	375	0.0	17.065575	31
ATCGTTT	405	0.0	16.98725	29
CATCGTT	410	0.0	16.389854	28
TAGAACA	325	0.0	16.242508	4
TAGCGGC	475	0.0	16.167387	30
AAACGCT	410	0.0	15.999619	29
CGCTTCG	400	0.0	15.998978	32
TCATAAC	60	0.0024437576	15.998336	3
CGGCATC	425	0.0	15.811389	25
AACGCTT	410	0.0	15.608758	30
GTCCTAC	175	3.6379788E-12	15.553094	1
GGCATCG	435	0.0	15.447907	26
GTCCTAT	520	0.0	15.394748	1
TCGTTTA	465	0.0	15.138818	30
GCATCGT	445	0.0	15.100763	27
GTTATTC	265	0.0	15.092769	3
>>END_MODULE