FastQCFastQC Report
Thu 2 Feb 2017
SRR4062979_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062979_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1246676
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT57800.46363289258796997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT39840.31956980001219243No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG25040.20085411125264305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT24400.19572045984682468No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA22940.18400931757730157No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC21180.16989177621130108No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC20470.16419663168297136No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA20310.16291321883151677No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT20110.16130895276719853No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG20060.160907886251119No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG19880.15946404679323256No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC19640.15753892751605067No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG18450.1479935444333572No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA18030.14462458569828887No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC17340.13908986777639099No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT17050.13676368198312952No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG17030.13660325537669774No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG16590.1330738700351976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15980.128180858539027No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT15800.12673701908114057No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT15640.125453606229686No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG15580.12497232641039051No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC15210.12200443419140176No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC15100.12112208785602675No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT14860.11919696857884486No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA14850.11911675527562894No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT14780.11855526215311758No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT13870.11125585156046959No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG13600.10909009237363998No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG13420.10764625291575358No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13340.10700454649002628No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATA400.004483720819.9999772
TCTAGCG4750.017.17823628
TAGCGGC4850.016.82404730
AACGAAT1252.8558134E-916.6393131
CTAGCGG5000.016.31932429
GTATTAG3100.016.0031911
GCTCTAA805.6118937E-516.003191
TAATACC1102.018678E-716.0006224
TTAGACC703.6956187E-416.0006224
CCAACGA1304.803951E-915.99933829
ATACCGT5050.015.8434726
TACCGTC4950.015.840277
TCTAGAT3150.015.7460122
ACCGTCG5250.015.5440858
CGTCGTA5150.015.53458310
GATATAC2900.015.4513541
GGACCGT1255.1300958E-815.3618296
GTACTAT859.4171875E-515.0618251
GACTATA859.4171875E-515.0618251
CCGATAA2800.014.8583159
Produced by FastQC (version 0.11.5)