##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1246676
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.17286047056332	32.0	32.0	32.0	32.0	32.0
2	31.292463318456438	32.0	32.0	32.0	32.0	32.0
3	31.37378757592189	32.0	32.0	32.0	32.0	32.0
4	31.476364348074398	32.0	32.0	32.0	32.0	32.0
5	31.40063577064129	32.0	32.0	32.0	32.0	32.0
6	34.91659741584822	36.0	36.0	36.0	36.0	36.0
7	34.93362910652006	36.0	36.0	36.0	36.0	36.0
8	34.88768132217192	36.0	36.0	36.0	32.0	36.0
9	34.97635953527621	36.0	36.0	36.0	36.0	36.0
10	34.82279196840238	36.0	36.0	36.0	32.0	36.0
11	35.002640621941865	36.0	36.0	36.0	36.0	36.0
12	34.89177941983322	36.0	36.0	36.0	32.0	36.0
13	34.947198791025095	36.0	36.0	36.0	36.0	36.0
14	34.88783854024622	36.0	36.0	36.0	32.0	36.0
15	34.870355248677285	36.0	36.0	36.0	32.0	36.0
16	34.86742345244474	36.0	36.0	36.0	32.0	36.0
17	34.82603739865049	36.0	36.0	36.0	32.0	36.0
18	34.82169224401529	36.0	36.0	36.0	32.0	36.0
19	34.816447096118	36.0	36.0	36.0	32.0	36.0
20	34.800367537355335	36.0	36.0	36.0	32.0	36.0
21	34.79481276610763	36.0	36.0	36.0	32.0	36.0
22	34.77031722757156	36.0	36.0	36.0	32.0	36.0
23	34.71138772223096	36.0	36.0	36.0	32.0	36.0
24	34.683564935877484	36.0	36.0	36.0	32.0	36.0
25	34.663358402664365	36.0	36.0	36.0	32.0	36.0
26	34.59700836464326	36.0	36.0	36.0	32.0	36.0
27	34.59281080248597	36.0	36.0	36.0	32.0	36.0
28	34.5546044040312	36.0	36.0	36.0	32.0	36.0
29	34.504297026653276	36.0	36.0	36.0	32.0	36.0
30	34.49167145272709	36.0	36.0	36.0	32.0	36.0
31	34.487753032864994	36.0	36.0	36.0	32.0	36.0
32	34.45268939163022	36.0	36.0	36.0	32.0	36.0
33	34.407871812724395	36.0	36.0	36.0	32.0	36.0
34	34.40092453853287	36.0	36.0	36.0	32.0	36.0
35	34.3598922254058	36.0	36.0	36.0	32.0	36.0
36	34.32857614969728	36.0	36.0	36.0	32.0	36.0
37	34.32602456452198	36.0	36.0	36.0	32.0	36.0
38	33.872867529334	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	5.0
20	8.0
21	40.0
22	107.0
23	329.0
24	932.0
25	2269.0
26	4768.0
27	9052.0
28	15801.0
29	24237.0
30	36312.0
31	51006.0
32	73321.0
33	116167.0
34	279251.0
35	633069.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.25922015912566	18.50056395109108	11.922792448349941	26.31742344143332
2	15.998944392929678	21.210964195989977	36.23018330344051	26.559908107639835
3	17.720482306549577	25.512883860762543	28.68900981489978	28.0776240177881
4	11.960374596426494	16.208153688812242	36.11374255519683	35.71772915956444
5	14.198797442158186	36.97568574352919	32.66750944110579	16.158007373206832
6	33.65207528523942	36.19332109809156	16.61848505460939	13.536118562059626
7	29.589513808006622	30.747320056342186	21.199549519198374	18.463616616452818
8	28.272718740750186	31.91617193961081	19.67076485965955	20.14034445997945
9	28.114825043898456	13.404490677644556	18.46807425156041	40.01261002689658
10	16.342160885241825	26.10444666369875	30.476270690201456	27.07712176085797
11	38.07310196010798	20.8182959454202	22.19009076579377	18.91851132867806
12	25.309624343853077	23.769764621327823	28.121029428751104	22.799581606067996
13	29.626349791525293	18.906305898735354	25.270359372217598	26.196984937521755
14	24.34441667281635	19.857364704221467	24.61313123858966	31.185087384372522
15	25.379729777424124	26.662340495846554	22.409350946035698	25.54857878069362
16	26.13349418774405	25.382136176520603	23.386509405811935	25.097860229923413
17	24.18832158475819	25.315158068335318	24.86091013222361	25.635610214682885
18	25.57817748958029	23.74586500421922	26.284455624396397	24.391501881804093
19	26.007960368211148	24.195701208654054	25.387510467836073	24.408827955298733
20	26.1710340136491	23.262660065646568	24.862193545075066	25.704112375629272
21	28.30791641132098	23.33790014406309	24.003028854329433	24.351154590286487
22	26.56183322691702	23.436803146928312	24.870054448790224	25.131309177364447
23	24.36855885108513	23.089714054699233	25.763793523877972	26.77793357033767
24	25.127298512203648	24.62051086248552	25.074197305474716	25.177993319836105
25	25.17947755387535	23.67788210871487	25.373834699368082	25.7688056380417
26	24.9591714286631	24.99021397700766	25.803657084920218	24.24695750940902
27	25.892453211580236	23.80746882108904	24.772033792260377	25.528044175070345
28	24.496821948926588	24.01778810212116	26.01397636595234	25.471413582999915
29	24.55834555249319	24.115568118741358	25.800207912881934	25.52587841588352
30	24.31618159008435	24.551286781810187	26.23392124337037	24.898610384735086
31	25.35414173369825	24.334390009914365	24.48454931353455	25.826918942852835
32	25.027513163003057	24.2120647225101	24.575511199381395	26.184910915105448
33	24.438907944004697	23.572524055969634	25.64940690283602	26.339161097189645
34	25.54857878069362	23.94126461085318	25.57488874414844	24.93526786430476
35	26.583330392178883	23.616641372738385	25.531814200321495	24.268214034761236
36	24.76144563623588	24.918503283932633	24.685162784877544	25.63488829495394
37	25.95686449406261	24.720697278202195	24.763771822029142	24.558666405706052
38	24.773337076623818	24.14205787394469	25.418092125052638	25.66651292437885
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	604.0
1	401.5
2	199.0
3	199.0
4	698.0
5	1197.0
6	1197.0
7	1450.5
8	1704.0
9	1673.5
10	1643.0
11	1643.0
12	2105.0
13	2567.0
14	3068.5
15	3570.0
16	3570.0
17	5541.5
18	7513.0
19	7513.0
20	8559.0
21	9605.0
22	9308.0
23	9011.0
24	9011.0
25	10002.0
26	10993.0
27	10993.0
28	14365.0
29	17737.0
30	21736.0
31	25735.0
32	25735.0
33	33620.5
34	41506.0
35	41506.0
36	45258.5
37	49011.0
38	56806.0
39	64601.0
40	64601.0
41	67663.0
42	70725.0
43	82375.0
44	94025.0
45	94025.0
46	94348.0
47	94671.0
48	94671.0
49	103658.5
50	112646.0
51	113966.5
52	115287.0
53	115287.0
54	110823.5
55	106360.0
56	106360.0
57	105175.5
58	103991.0
59	93357.5
60	82724.0
61	82724.0
62	80962.5
63	79201.0
64	65516.5
65	51832.0
66	51832.0
67	43519.5
68	35207.0
69	35207.0
70	28229.5
71	21252.0
72	16512.0
73	11772.0
74	11772.0
75	8719.0
76	5666.0
77	5666.0
78	5884.5
79	6103.0
80	4668.5
81	3234.0
82	3234.0
83	3238.5
84	3243.0
85	3243.0
86	2027.0
87	811.0
88	692.5
89	574.0
90	574.0
91	329.5
92	85.0
93	61.0
94	37.0
95	37.0
96	25.5
97	14.0
98	14.0
99	17.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00914431656661394
2	0.0
3	0.0
4	8.021330321591176E-5
5	0.0
6	3.20853212863647E-4
7	6.41706425727294E-4
8	0.002326185793261441
9	0.003930451857579676
10	8.823463353750293E-4
11	0.004893011496170617
12	3.20853212863647E-4
13	1.604266064318235E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	3.20853212863647E-4
24	0.0
25	1.604266064318235E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	8.021330321591176E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1246676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.654511723444166
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26245969708917	33.608083888789125
2	14.696043696401645	13.124893110584301
3	4.63299106908222	6.206518620268322
4	1.8908820158239665	3.377456525730442
5	0.9951295300839652	2.2218511633739952
6	0.5459653112419618	1.4627888634868818
7	0.36983484462295113	1.1560356083467607
8	0.2427018962191083	0.8670187736014641
9	0.1764388284382929	0.7090910759671655
>10	0.9220191407581478	7.619005562908078
>50	0.10405789357830601	3.303274095988767
>100	0.13077033662402482	12.862998909662307
>500	0.021313396025090504	6.66741768037859
>1k	0.0092117220108442	6.347375179250638
>5k	1.8062200021263142E-4	0.46619094166319786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	5780	0.46363289258796997	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3984	0.31956980001219243	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	2504	0.20085411125264305	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	2440	0.19572045984682468	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	2294	0.18400931757730157	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	2118	0.16989177621130108	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	2047	0.16419663168297136	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	2031	0.16291321883151677	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	2011	0.16130895276719853	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	2006	0.160907886251119	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1988	0.15946404679323256	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1964	0.15753892751605067	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1845	0.1479935444333572	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1803	0.14462458569828887	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1734	0.13908986777639099	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	1705	0.13676368198312952	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1703	0.13660325537669774	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1659	0.1330738700351976	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1598	0.128180858539027	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	1580	0.12673701908114057	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	1564	0.125453606229686	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	1558	0.12497232641039051	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1521	0.12200443419140176	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	1510	0.12112208785602675	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	1486	0.11919696857884486	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	1485	0.11911675527562894	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	1478	0.11855526215311758	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	1387	0.11125585156046959	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	1360	0.10909009237363998	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	1342	0.10764625291575358	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1334	0.10700454649002628	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	8.021330321591176E-5	0.0
5	0.0	0.0	0.0	8.021330321591176E-5	0.0
6	0.0	0.0	0.0	8.021330321591176E-5	1.604266064318235E-4
7	0.0	0.0	0.0	1.604266064318235E-4	1.604266064318235E-4
8	0.0	0.0	0.0	1.604266064318235E-4	1.604266064318235E-4
9	0.0	0.0	0.0	1.604266064318235E-4	1.604266064318235E-4
10	0.0	0.0	0.0	2.4063990964773527E-4	1.604266064318235E-4
11	0.0	0.0	0.0	2.4063990964773527E-4	1.604266064318235E-4
12	0.0	0.0	0.0	2.4063990964773527E-4	3.20853212863647E-4
13	0.0	0.0	0.0	2.4063990964773527E-4	4.0106651607955875E-4
14	0.0	0.0	0.0	2.4063990964773527E-4	4.0106651607955875E-4
15	0.0	0.0	0.0	2.4063990964773527E-4	6.41706425727294E-4
16	0.0	0.0	0.0	2.4063990964773527E-4	6.41706425727294E-4
17	0.0	0.0	0.0	2.4063990964773527E-4	6.41706425727294E-4
18	0.0	0.0	0.0	4.0106651607955875E-4	6.41706425727294E-4
19	0.0	0.0	0.0	4.0106651607955875E-4	6.41706425727294E-4
20	0.0	0.0	0.0	4.8127981929547053E-4	6.41706425727294E-4
21	0.0	0.0	0.0	5.614931225113823E-4	7.219197289432057E-4
22	0.0	0.0	0.0	0.0013636261546704999	9.625596385909411E-4
23	0.0	0.0	0.0	0.0026470390061250878	9.625596385909411E-4
24	0.0	0.0	0.0	0.004090878464011499	9.625596385909411E-4
25	0.0	0.0	0.0	0.0044117316768751465	9.625596385909411E-4
26	0.0	0.0	0.0	0.005374291315466087	0.0010427729418068527
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTATA	40	0.0044837208	19.999977	2
TCTAGCG	475	0.0	17.178236	28
TAGCGGC	485	0.0	16.824047	30
AACGAAT	125	2.8558134E-9	16.63931	31
CTAGCGG	500	0.0	16.319324	29
GTATTAG	310	0.0	16.003191	1
GCTCTAA	80	5.6118937E-5	16.00319	1
TAATACC	110	2.018678E-7	16.000622	4
TTAGACC	70	3.6956187E-4	16.000622	4
CCAACGA	130	4.803951E-9	15.999338	29
ATACCGT	505	0.0	15.843472	6
TACCGTC	495	0.0	15.84027	7
TCTAGAT	315	0.0	15.746012	2
ACCGTCG	525	0.0	15.544085	8
CGTCGTA	515	0.0	15.534583	10
GATATAC	290	0.0	15.451354	1
GGACCGT	125	5.1300958E-8	15.361829	6
GTACTAT	85	9.4171875E-5	15.061825	1
GACTATA	85	9.4171875E-5	15.061825	1
CCGATAA	280	0.0	14.858315	9
>>END_MODULE