FastQCFastQC Report
Thu 2 Feb 2017
SRR4062978_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062978_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692993
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT33100.47763830226279347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT22330.32222547702502047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT13300.19192113051646986No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12670.18283012959726866No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC12490.18023270076321118No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT12290.17734666872536953No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG12240.1766251607159091No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG11860.17114169984400995No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11670.1683999694080604No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC11380.16421522295319No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11120.16046338130399584No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10980.1584431588775067No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10970.1582988572756146No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10870.1568558412566938No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG10740.1549799204320967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10680.15411411082074422No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10140.14632182431857174No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA9500.13708652179747846No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT9460.13650931538991015No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9420.13593210898234181No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA9420.13593210898234181No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA9070.13088155291611894No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG8640.12467658403475937No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8510.12280066321016231No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT8470.12222345680259397No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC8130.11731720233826316No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC8090.11673999593069483No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT7850.11327675748528485No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG7840.11313245588339277No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7810.11269955107771651No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG7590.1095249158360907No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT7500.10822620141906195No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7410.10692748700203321No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7380.10649458219635696No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT7290.10519586777932823No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7120.10274274054716281No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC7120.10274274054716281No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG6960.10043391491688948No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAA400.004443648320.0294971
TCGGACA400.00448282419.99914420
CAATACT400.00448469719.9977024
ATACCGT1900.019.3661946
TACCGTC2000.018.3978847
GTCCTAG450.0087720317.8039951
CCTTTAG450.0087720317.8039951
AAAAACG450.00884533117.778315
GTAGGAC904.365811E-717.7757343
TTAAGTC450.00885268917.7757343
ACCGTCG2000.017.5979778
GCCGTTA550.001363636617.45505715
CTAGCGG2950.017.35393529
AACGCTT2550.016.9404530
AATACTT957.6907963E-716.8401685
TAGCGGC3050.016.78616530
CGCTTCG2600.016.61467432
TCTAGCG3100.016.5142328
AAACGCT2650.016.30001329
CCGTTAA600.00244011516.0004716
Produced by FastQC (version 0.11.5)