##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062978_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692993
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138857102452693	32.0	32.0	32.0	32.0	32.0
2	30.694843959462794	32.0	32.0	32.0	32.0	32.0
3	30.771727852950896	32.0	32.0	32.0	32.0	32.0
4	30.837965174251398	32.0	32.0	32.0	32.0	32.0
5	30.744692947836413	32.0	32.0	32.0	32.0	32.0
6	34.385214569266935	36.0	36.0	36.0	32.0	36.0
7	34.273239412230716	36.0	36.0	36.0	32.0	36.0
8	34.251044382843695	36.0	36.0	36.0	32.0	36.0
9	34.3810327088441	36.0	36.0	36.0	32.0	36.0
10	34.091443925119016	36.0	36.0	36.0	32.0	36.0
11	34.41095797504448	36.0	36.0	36.0	32.0	36.0
12	34.18992255333026	36.0	36.0	36.0	32.0	36.0
13	34.30865391136707	36.0	36.0	36.0	32.0	36.0
14	34.18806683472993	36.0	36.0	36.0	32.0	36.0
15	34.13704756036497	36.0	36.0	36.0	32.0	36.0
16	34.156773589343615	36.0	36.0	36.0	32.0	36.0
17	34.04177387073174	36.0	36.0	36.0	32.0	36.0
18	34.09443385431022	36.0	36.0	36.0	32.0	36.0
19	34.08654055668672	36.0	36.0	36.0	32.0	36.0
20	34.04355166646705	36.0	36.0	36.0	32.0	36.0
21	34.02293385358871	36.0	36.0	36.0	32.0	36.0
22	34.0054531575355	36.0	36.0	36.0	32.0	36.0
23	33.9467584809659	36.0	36.0	36.0	32.0	36.0
24	33.92822149718684	36.0	36.0	36.0	32.0	36.0
25	33.899756563197606	36.0	36.0	36.0	32.0	36.0
26	33.856207782762596	36.0	36.0	36.0	32.0	36.0
27	33.876050696038774	36.0	36.0	36.0	32.0	36.0
28	33.86651668920177	36.0	36.0	36.0	32.0	36.0
29	33.81509625638354	36.0	36.0	36.0	32.0	36.0
30	33.782475436259816	36.0	36.0	36.0	32.0	36.0
31	33.813809086094665	36.0	36.0	36.0	32.0	36.0
32	33.74284300130016	36.0	36.0	36.0	32.0	36.0
33	33.69692911761013	36.0	36.0	36.0	27.0	36.0
34	33.68674575356461	36.0	36.0	36.0	27.0	36.0
35	33.63638882355233	36.0	36.0	36.0	27.0	36.0
36	33.60207534563841	36.0	36.0	36.0	27.0	36.0
37	33.60339426227971	36.0	36.0	36.0	27.0	36.0
38	32.97765778297905	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	2.0
10	9.0
11	4.0
12	3.0
13	0.0
14	183.0
15	308.0
16	354.0
17	435.0
18	514.0
19	679.0
20	906.0
21	1186.0
22	1784.0
23	2708.0
24	3848.0
25	5675.0
26	7975.0
27	10962.0
28	15178.0
29	20073.0
30	26802.0
31	35244.0
32	47696.0
33	70884.0
34	151950.0
35	287629.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.53413277468757	17.246984035252474	11.408798670808352	24.810084519251607
2	16.92877232903453	20.20571877400389	36.761113260833156	26.10439563612842
3	18.704692300920335	25.006746995602548	28.46741453685303	27.821146166624093
4	12.405245226004476	15.455003297386765	35.79259220777721	36.34715926883154
5	14.849132022107101	36.55045527352487	32.58315415806866	16.01725854629937
6	33.682832821782675	35.54389595247269	16.98420325805097	13.789067967693663
7	30.077504390376237	30.891509726649474	20.823731264240763	18.207254618733522
8	28.816247252850324	31.34716035273185	19.797948313903067	20.038644080514754
9	27.913309244551165	14.013148780938309	18.92561530291836	39.147926671592174
10	16.835596473253585	25.746114662549243	29.917314824167736	27.500974040029437
11	37.54345357232016	21.45643242744029	21.86914215541263	19.130971844826913
12	25.472817161971445	24.02139185597151	27.657026093331062	22.848764888725984
13	29.5878517432379	18.808467526619772	25.547765497773145	26.05591523236918
14	24.470010303952158	20.091407889306115	24.668007815995082	30.770573990746648
15	25.487524482670526	26.485739069939974	22.951430134360606	25.075306313028893
16	26.222242747071476	25.500233069284356	23.542306765359122	24.735217418285043
17	23.8620323356301	25.627329120829955	25.131123549125505	25.379514994414436
18	25.502085981998935	23.76850578461352	26.610953894819172	24.11845433856837
19	25.774051331635267	24.656646631406534	25.709832670702582	23.85946936625562
20	26.108941667460368	23.18697525679767	25.30658423742853	25.39749883831343
21	28.07896062214898	23.52468349224262	24.14261861915599	24.253737266452415
22	26.364422201345267	23.601592613597827	24.929828890726448	25.104156294330465
23	24.089381991987715	23.4558458501218	25.778138240761052	26.676633917129433
24	24.70868896323001	25.1977913182528	25.282074521360887	24.811445197156306
25	25.100665910409504	23.901334418648958	25.420631965431546	25.577367705509996
26	24.657370149925818	25.23164318002067	25.892656117400513	24.218330552653
27	25.536950476303176	24.46355461720406	24.849013119443068	25.15048178704969
28	24.283976396871964	24.256413438601708	26.26086103218228	25.19874913234405
29	24.111486491362005	24.734351526206947	26.21964507083719	24.934516911593864
30	24.229012590641798	24.99816010678596	26.37555467368953	24.397272628882718
31	25.061836359490762	24.89746853344647	24.443905691254013	25.59678941580875
32	24.401007280415037	24.925428058099012	24.548347295278912	26.125217366207043
33	24.096528116512587	24.272445731075507	25.615423024859368	26.015603127552538
34	25.259914564451886	24.49907637245281	25.656497142527275	24.58451192056803
35	26.266210993312107	24.365776006142355	25.46810911914377	23.899903881401773
36	24.369348891246148	25.563036246473132	24.730298241436294	25.33731662084443
37	25.734122631815925	25.354289573640276	24.84313125598901	24.068456538554788
38	24.43279063256724	24.801790595046715	25.603719727427794	25.16169904495825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1193.0
1	810.0
2	427.0
3	427.0
4	779.0
5	1131.0
6	1131.0
7	1243.5
8	1356.0
9	1284.0
10	1212.0
11	1212.0
12	1460.0
13	1708.0
14	2142.0
15	2576.0
16	2576.0
17	3637.0
18	4698.0
19	4698.0
20	5329.0
21	5960.0
22	5670.0
23	5380.0
24	5380.0
25	5809.0
26	6238.0
27	6238.0
28	7991.0
29	9744.0
30	12032.0
31	14320.0
32	14320.0
33	18422.0
34	22524.0
35	22524.0
36	24416.0
37	26308.0
38	30691.0
39	35074.0
40	35074.0
41	37221.5
42	39369.0
43	45936.5
44	52504.0
45	52504.0
46	54342.5
47	56181.0
48	56181.0
49	59537.5
50	62894.0
51	63539.5
52	64185.0
53	64185.0
54	60580.0
55	56975.0
56	56975.0
57	56243.5
58	55512.0
59	49822.0
60	44132.0
61	44132.0
62	43503.5
63	42875.0
64	35363.0
65	27851.0
66	27851.0
67	23279.0
68	18707.0
69	18707.0
70	15199.5
71	11692.0
72	9228.0
73	6764.0
74	6764.0
75	4982.5
76	3201.0
77	3201.0
78	3404.5
79	3608.0
80	2858.5
81	2109.0
82	2109.0
83	2191.5
84	2274.0
85	2274.0
86	1519.5
87	765.0
88	664.0
89	563.0
90	563.0
91	404.5
92	246.0
93	215.5
94	185.0
95	185.0
96	172.5
97	160.0
98	160.0
99	276.0
100	392.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12164625039502563
2	0.05728773595115679
3	0.013275747374071598
4	0.002886032037841652
5	4.329048056762478E-4
6	2.8860320378416523E-4
7	0.0
8	2.8860320378416523E-4
9	1.4430160189208262E-4
10	4.329048056762478E-4
11	0.0017316192227049912
12	0.0027417304359495694
13	0.012554239364611185
14	0.007936588104064543
15	0.02251104989516489
16	0.009523905724877452
17	0.01948071625543115
18	0.004906254464330809
19	0.006926476890819965
20	0.005050556066222891
21	0.005772064075683304
22	0.006349270483251635
23	0.008225191307848708
24	0.013131445772179517
25	0.015440271402452837
26	0.016883287421373665
27	0.0075036832983882956
28	0.004906254464330809
29	0.009523905724877452
30	0.002597428834057487
31	0.005050556066222891
32	0.005483460871899139
33	0.007936588104064543
34	0.01053401693812203
35	0.014285858587316177
36	0.014141556985424096
37	0.008225191307848708
38	0.005627762473791222
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	692993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.11259100236789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.76397659541945	39.66528150255649
2	11.56486331847887	11.359608043174797
3	3.2933725519473107	4.852381774866386
4	1.3376398228317308	2.6277983010885855
5	0.7165580949228629	1.759601232269124
6	0.4506418989142285	1.3279314761942953
7	0.30262958833024284	1.040404623781473
8	0.20102273736434784	0.7898197985881307
9	0.1689305699043192	0.7466956188756974
>10	0.9122363386838698	8.821855426831172
>50	0.1248753649838066	4.401227133286641
>100	0.14435944811766452	14.703426274381249
>500	0.013875038980633919	4.586230228288754
>1k	0.005018631120654822	3.3177385658172596
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	3310	0.47763830226279347	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2233	0.32222547702502047	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1330	0.19192113051646986	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1267	0.18283012959726866	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1249	0.18023270076321118	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1229	0.17734666872536953	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1224	0.1766251607159091	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1186	0.17114169984400995	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1167	0.1683999694080604	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1138	0.16421522295319	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1112	0.16046338130399584	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1098	0.1584431588775067	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1097	0.1582988572756146	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1087	0.1568558412566938	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1074	0.1549799204320967	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1068	0.15411411082074422	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1014	0.14632182431857174	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	950	0.13708652179747846	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	946	0.13650931538991015	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	942	0.13593210898234181	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	942	0.13593210898234181	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	907	0.13088155291611894	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	864	0.12467658403475937	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	851	0.12280066321016231	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	847	0.12222345680259397	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	813	0.11731720233826316	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	809	0.11673999593069483	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	785	0.11327675748528485	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	784	0.11313245588339277	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	781	0.11269955107771651	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTG	759	0.1095249158360907	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	750	0.10822620141906195	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	741	0.10692748700203321	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	738	0.10649458219635696	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATT	729	0.10519586777932823	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	712	0.10274274054716281	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	712	0.10274274054716281	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	696	0.10043391491688948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4430160189208262E-4
2	0.0	0.0	0.0	0.0	1.4430160189208262E-4
3	0.0	0.0	0.0	0.0	1.4430160189208262E-4
4	0.0	0.0	0.0	0.0	1.4430160189208262E-4
5	0.0	0.0	0.0	0.0	1.4430160189208262E-4
6	0.0	0.0	0.0	0.0	1.4430160189208262E-4
7	0.0	0.0	0.0	0.0	1.4430160189208262E-4
8	0.0	0.0	0.0	0.0	1.4430160189208262E-4
9	0.0	0.0	0.0	0.0	1.4430160189208262E-4
10	0.0	0.0	0.0	0.0	1.4430160189208262E-4
11	0.0	0.0	0.0	0.0	1.4430160189208262E-4
12	0.0	0.0	0.0	0.0	4.329048056762478E-4
13	0.0	0.0	0.0	0.0	7.21508009460413E-4
14	0.0	0.0	0.0	0.0	7.21508009460413E-4
15	0.0	0.0	0.0	2.8860320378416523E-4	8.658096113524956E-4
16	0.0	0.0	0.0	2.8860320378416523E-4	8.658096113524956E-4
17	0.0	0.0	0.0	2.8860320378416523E-4	8.658096113524956E-4
18	0.0	0.0	0.0	2.8860320378416523E-4	8.658096113524956E-4
19	0.0	0.0	0.0	2.8860320378416523E-4	8.658096113524956E-4
20	0.0	0.0	0.0	2.8860320378416523E-4	8.658096113524956E-4
21	0.0	0.0	0.0	8.658096113524956E-4	8.658096113524956E-4
22	0.0	0.0	0.0	0.001154412815136661	8.658096113524956E-4
23	0.0	0.0	0.0	0.0033189368435179	8.658096113524956E-4
24	0.0	0.0	0.0	0.006782175288927882	8.658096113524956E-4
25	0.0	0.0	0.0	0.007936588104064543	8.658096113524956E-4
26	0.0	0.0	0.0	0.009668207326769535	8.658096113524956E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTCTAA	40	0.0044436483	20.029497	1
TCGGACA	40	0.004482824	19.999144	20
CAATACT	40	0.004484697	19.997702	4
ATACCGT	190	0.0	19.366194	6
TACCGTC	200	0.0	18.397884	7
GTCCTAG	45	0.00877203	17.803995	1
CCTTTAG	45	0.00877203	17.803995	1
AAAAACG	45	0.008845331	17.7783	15
GTAGGAC	90	4.365811E-7	17.775734	3
TTAAGTC	45	0.008852689	17.775734	3
ACCGTCG	200	0.0	17.597977	8
GCCGTTA	55	0.0013636366	17.455057	15
CTAGCGG	295	0.0	17.353935	29
AACGCTT	255	0.0	16.94045	30
AATACTT	95	7.6907963E-7	16.840168	5
TAGCGGC	305	0.0	16.786165	30
CGCTTCG	260	0.0	16.614674	32
TCTAGCG	310	0.0	16.51423	28
AAACGCT	265	0.0	16.300013	29
CCGTTAA	60	0.002440115	16.00047	16
>>END_MODULE