FastQCFastQC Report
Thu 2 Feb 2017
SRR4062978_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062978_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences692993
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT42600.614724824060272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT32220.4649397612962901No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT19250.277780583642259No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT13630.1966830833789086No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC13590.19610587697134027No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13290.19177682891457778No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA12670.18283012959726866No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC12360.1783567799386141No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA11810.17042019183454957No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT11670.1683999694080604No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC11390.1643595245550821No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA11240.16219500052670086No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG11130.16060768290588795No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10740.1549799204320967No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG10710.15454701562642045No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG10700.15440271402452838No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG10380.14978506276398176No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10360.14949645956019758No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC10320.14891925315262924No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG9790.14127126825234887No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT9320.134489092963421No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG9300.13420048975963683No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT8650.12482088563665146No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT8650.12482088563665146No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA8360.12063613918178107No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT8360.12063613918178107No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT8090.11673999593069483No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC7840.11313245588339277No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT7700.1111122334569036No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT7480.10793759821527779No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG7390.10663888379824904No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG7190.1037528517604074No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7070.10202123253770241No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT6940.10014531171310534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC308.408158E-426.6695543
GCGACGC552.2171134E-623.27188932
TAGGACG350.002064418322.8596154
CTTATTC453.5322283E-421.3356423
GCCCATA400.004477037620.0036091
CTAGAAC400.00447890820.0021633
CCTAATA400.00447890820.0021632
TACTATT400.00447890820.0021637
TCGTGAA400.004482650719.99927713
TAGGGTC902.0323569E-819.557674
TATAGAC507.186758E-419.2020782
CGTCTTA751.5073219E-619.19930815
TAAACGC3350.018.62619428
GTATAGA701.782822E-518.2890131
TATACCG450.00884131317.7797015
TCTAGCG2250.017.77713828
CCATAGG1201.6516424E-917.336461
AAACGCT3600.017.33270629
TAGCGGC2250.017.06605130
CTAGCGG2250.017.06605129
Produced by FastQC (version 0.11.5)