##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062978_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	692993
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.118817938997942	32.0	32.0	32.0	32.0	32.0
2	31.285916307956935	32.0	32.0	32.0	32.0	32.0
3	31.370487147777826	32.0	32.0	32.0	32.0	32.0
4	31.465964302669725	32.0	32.0	32.0	32.0	32.0
5	31.403079107581174	32.0	32.0	32.0	32.0	32.0
6	34.90965709610342	36.0	36.0	36.0	36.0	36.0
7	34.910520019682735	36.0	36.0	36.0	36.0	36.0
8	34.85787446626445	36.0	36.0	36.0	32.0	36.0
9	34.96008906294869	36.0	36.0	36.0	36.0	36.0
10	34.81879903548809	36.0	36.0	36.0	32.0	36.0
11	34.987118195999095	36.0	36.0	36.0	36.0	36.0
12	34.87669572419924	36.0	36.0	36.0	32.0	36.0
13	34.93040766645551	36.0	36.0	36.0	36.0	36.0
14	34.86001157298847	36.0	36.0	36.0	32.0	36.0
15	34.83749186499719	36.0	36.0	36.0	32.0	36.0
16	34.8440056393066	36.0	36.0	36.0	32.0	36.0
17	34.81072824689427	36.0	36.0	36.0	32.0	36.0
18	34.78414211976167	36.0	36.0	36.0	32.0	36.0
19	34.791435122721296	36.0	36.0	36.0	32.0	36.0
20	34.77153448880436	36.0	36.0	36.0	32.0	36.0
21	34.77174084009506	36.0	36.0	36.0	32.0	36.0
22	34.74427303017491	36.0	36.0	36.0	32.0	36.0
23	34.678272363501506	36.0	36.0	36.0	32.0	36.0
24	34.64447115627431	36.0	36.0	36.0	32.0	36.0
25	34.63399341696092	36.0	36.0	36.0	32.0	36.0
26	34.56489170886286	36.0	36.0	36.0	32.0	36.0
27	34.55326821483045	36.0	36.0	36.0	32.0	36.0
28	34.51631113156987	36.0	36.0	36.0	32.0	36.0
29	34.46557468834462	36.0	36.0	36.0	32.0	36.0
30	34.44766541653379	36.0	36.0	36.0	32.0	36.0
31	34.44294386811988	36.0	36.0	36.0	32.0	36.0
32	34.37880036306283	36.0	36.0	36.0	32.0	36.0
33	34.3214159450384	36.0	36.0	36.0	32.0	36.0
34	34.304806830660624	36.0	36.0	36.0	32.0	36.0
35	34.24515254843844	36.0	36.0	36.0	32.0	36.0
36	34.20996604583308	36.0	36.0	36.0	32.0	36.0
37	34.2124999819623	36.0	36.0	36.0	32.0	36.0
38	33.76534539309921	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	4.0
20	6.0
21	28.0
22	86.0
23	225.0
24	640.0
25	1401.0
26	2925.0
27	5514.0
28	9370.0
29	14119.0
30	20669.0
31	29343.0
32	41410.0
33	65177.0
34	154590.0
35	347484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37930317873111	18.592387555237416	12.277787559278298	25.750521706753183
2	15.911704735834272	21.302812582522478	36.12633893848856	26.659143743154694
3	17.68473851828229	25.441671127991196	28.97042250066018	27.903167853066336
4	11.927987624676764	16.18200498707056	36.0040231344662	35.88598425378648
5	14.130301460476513	36.92447109855366	32.555162894863294	16.390064546106526
6	33.598944865164384	36.09666489656446	16.456178426302177	13.84821181196897
7	29.60345748134894	31.112137260277926	21.009538377177158	18.27486688119598
8	28.666999529566596	31.436351514766702	19.71014952855646	20.186499427110242
9	27.97539850439993	13.65738001575835	18.6493998262541	39.71782165358762
10	16.6602451712519	25.78295345498099	30.204261275496584	27.352540098270527
11	37.62428531434624	21.20260562628254	22.047096377579145	19.126012681792076
12	25.2733440982639	23.991364967221795	27.79199152658548	22.94329940792882
13	29.4799939970447	18.850001154414482	25.632330531954196	26.037674316586628
14	24.396494625486838	19.904818663391982	24.793324030690066	30.905362680431114
15	25.6474452122893	26.434610450610613	22.77526612822929	25.142678208870795
16	26.081937335586364	25.467501114729874	23.489414755993206	24.961146793690556
17	23.803992248117947	25.422479014939547	25.262448538441223	25.511080198501286
18	25.379477137575705	23.43154981363448	26.927544722673968	24.261428326115848
19	25.658123530829315	24.476726316138837	26.10040794062855	23.7647422124033
20	25.996366485664357	22.912641253230554	25.693044518487202	25.397947742617895
21	27.915842941456585	23.420972885611626	24.569330004761973	24.093854168169816
22	26.055674444042005	23.391722571512265	25.44066101677795	25.111941967667782
23	24.02123548502073	22.91530481636847	26.38331522343003	26.68014447518077
24	24.562585769264626	24.711793625621038	25.747590524002405	24.97803008111193
25	24.951117691283148	23.50174821895234	25.971332961034125	25.575801128730387
26	24.726656690615922	24.741952660416484	26.365201380100522	24.166189268867075
27	25.689292676838004	23.969361883886272	25.330847497738073	25.01049794153765
28	24.17022971372005	23.857239539216124	26.863041906628204	25.109488840435617
29	24.265757374172612	24.157242569549766	26.67949026902148	24.897509787256148
30	24.190431937984943	24.39389719665278	26.965207440767802	24.450463424594478
31	25.114683698103736	24.12867085237513	25.11785833334536	25.638787116175777
32	24.467923918423416	24.392887085439536	25.125939223051315	26.013249773085732
33	23.99389315620793	23.81611358267688	26.107767322325042	26.08222593879015
34	25.221466883503872	23.905003369442404	26.241534907279007	24.631994839774716
35	26.310943977789098	23.80139481928389	25.888573189050973	23.99908801387604
36	24.45840001269854	25.070527407924754	25.382218868011652	25.08885371136505
37	25.75884604894999	24.717565689696723	25.419737284503597	24.103850976849696
38	24.563811074025494	24.22008366631024	25.983742934612426	25.232362325051838
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	683.0
1	474.0
2	265.0
3	265.0
4	705.0
5	1145.0
6	1145.0
7	1311.0
8	1477.0
9	1377.0
10	1277.0
11	1277.0
12	1572.0
13	1867.0
14	2352.0
15	2837.0
16	2837.0
17	4288.5
18	5740.0
19	5740.0
20	6158.0
21	6576.0
22	6204.0
23	5832.0
24	5832.0
25	5831.5
26	5831.0
27	5831.0
28	7670.5
29	9510.0
30	11573.0
31	13636.0
32	13636.0
33	18127.5
34	22619.0
35	22619.0
36	24389.5
37	26160.0
38	30634.5
39	35109.0
40	35109.0
41	37334.0
42	39559.0
43	45964.5
44	52370.0
45	52370.0
46	54439.5
47	56509.0
48	56509.0
49	60108.5
50	63708.0
51	63818.5
52	63929.0
53	63929.0
54	60427.0
55	56925.0
56	56925.0
57	56474.5
58	56024.0
59	49909.5
60	43795.0
61	43795.0
62	43223.0
63	42651.0
64	35176.0
65	27701.0
66	27701.0
67	23329.0
68	18957.0
69	18957.0
70	15350.5
71	11744.0
72	9239.0
73	6734.0
74	6734.0
75	4973.5
76	3213.0
77	3213.0
78	3449.0
79	3685.0
80	2840.0
81	1995.0
82	1995.0
83	1972.5
84	1950.0
85	1950.0
86	1249.0
87	548.0
88	437.0
89	326.0
90	326.0
91	191.5
92	57.0
93	41.5
94	26.0
95	26.0
96	18.5
97	11.0
98	11.0
99	11.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.010822620141906195
2	0.0
3	0.0
4	1.4430160189208262E-4
5	0.0
6	1.4430160189208262E-4
7	4.329048056762478E-4
8	0.0021645240283812393
9	0.0038961432510862303
10	0.001154412815136661
11	0.00447334965865456
12	2.8860320378416523E-4
13	1.4430160189208262E-4
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	4.329048056762478E-4
22	0.0
23	2.8860320378416523E-4
24	0.0
25	2.8860320378416523E-4
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	2.8860320378416523E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	692993.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.67312526135369
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.19545910127665	36.96299582764298
2	12.474953197663796	11.644901064481742
3	3.585061079632295	5.019780145178459
4	1.4691097501435355	2.74271773564501
5	0.7681087310429577	1.79250175091537
6	0.49200751536574405	1.3778117036515662
7	0.3154985665241142	1.0307712880610258
8	0.2244096530654385	0.8379119877904108
9	0.16068282077795643	0.6749612479365485
>10	0.9941389170360245	8.902518976175065
>50	0.14036638465002754	4.62210678471688
>100	0.15841792738539698	15.505606668163201
>500	0.01587291610344824	4.817194059785513
>1k	0.005913439332657188	4.068220759856254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4260	0.614724824060272	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3222	0.4649397612962901	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1925	0.277780583642259	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1363	0.1966830833789086	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1359	0.19610587697134027	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1329	0.19177682891457778	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1267	0.18283012959726866	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1236	0.1783567799386141	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1181	0.17042019183454957	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1167	0.1683999694080604	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1139	0.1643595245550821	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1124	0.16219500052670086	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1113	0.16060768290588795	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC	1074	0.1549799204320967	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1071	0.15454701562642045	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1070	0.15440271402452838	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG	1038	0.14978506276398176	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA	1036	0.14949645956019758	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1032	0.14891925315262924	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	979	0.14127126825234887	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCT	932	0.134489092963421	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCG	930	0.13420048975963683	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAAT	865	0.12482088563665146	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTT	865	0.12482088563665146	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTA	836	0.12063613918178107	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGT	836	0.12063613918178107	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT	809	0.11673999593069483	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCC	784	0.11313245588339277	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGT	770	0.1111122334569036	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	748	0.10793759821527779	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGG	739	0.10663888379824904	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAG	719	0.1037528517604074	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	707	0.10202123253770241	No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGT	694	0.10014531171310534	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4430160189208262E-4
2	0.0	0.0	0.0	0.0	1.4430160189208262E-4
3	0.0	0.0	0.0	0.0	1.4430160189208262E-4
4	0.0	0.0	0.0	0.0	1.4430160189208262E-4
5	0.0	0.0	0.0	0.0	1.4430160189208262E-4
6	0.0	0.0	0.0	0.0	1.4430160189208262E-4
7	0.0	0.0	0.0	0.0	1.4430160189208262E-4
8	0.0	0.0	0.0	0.0	1.4430160189208262E-4
9	0.0	0.0	0.0	0.0	1.4430160189208262E-4
10	0.0	0.0	0.0	1.4430160189208262E-4	1.4430160189208262E-4
11	0.0	0.0	0.0	1.4430160189208262E-4	1.4430160189208262E-4
12	0.0	0.0	0.0	1.4430160189208262E-4	2.8860320378416523E-4
13	0.0	0.0	0.0	1.4430160189208262E-4	4.329048056762478E-4
14	0.0	0.0	0.0	1.4430160189208262E-4	4.329048056762478E-4
15	0.0	0.0	0.0	4.329048056762478E-4	4.329048056762478E-4
16	0.0	0.0	0.0	4.329048056762478E-4	4.329048056762478E-4
17	0.0	0.0	0.0	4.329048056762478E-4	4.329048056762478E-4
18	0.0	0.0	0.0	4.329048056762478E-4	4.329048056762478E-4
19	0.0	0.0	0.0	4.329048056762478E-4	4.329048056762478E-4
20	0.0	0.0	0.0	4.329048056762478E-4	4.329048056762478E-4
21	0.0	0.0	0.0	0.0010101112132445782	4.329048056762478E-4
22	0.0	0.0	0.0	0.001154412815136661	4.329048056762478E-4
23	0.0	0.0	0.0	0.0031746352416258173	4.329048056762478E-4
24	0.0	0.0	0.0	0.0066378736870358	4.329048056762478E-4
25	0.0	0.0	0.0	0.0075036832983882956	4.329048056762478E-4
26	0.0	0.0	0.0	0.009523905724877452	4.329048056762478E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTATAC	30	8.408158E-4	26.669554	3
GCGACGC	55	2.2171134E-6	23.271889	32
TAGGACG	35	0.0020644183	22.859615	4
CTTATTC	45	3.5322283E-4	21.335642	3
GCCCATA	40	0.0044770376	20.003609	1
CTAGAAC	40	0.004478908	20.002163	3
CCTAATA	40	0.004478908	20.002163	2
TACTATT	40	0.004478908	20.002163	7
TCGTGAA	40	0.0044826507	19.999277	13
TAGGGTC	90	2.0323569E-8	19.55767	4
TATAGAC	50	7.186758E-4	19.202078	2
CGTCTTA	75	1.5073219E-6	19.199308	15
TAAACGC	335	0.0	18.626194	28
GTATAGA	70	1.782822E-5	18.289013	1
TATACCG	45	0.008841313	17.779701	5
TCTAGCG	225	0.0	17.777138	28
CCATAGG	120	1.6516424E-9	17.33646	1
AAACGCT	360	0.0	17.332706	29
TAGCGGC	225	0.0	17.066051	30
CTAGCGG	225	0.0	17.066051	29
>>END_MODULE