FastQCFastQC Report
Thu 2 Feb 2017
SRR4062977_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062977_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227483
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT26080.2124673009728037No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC17980.14647860703569826No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT17610.14346430867066998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT16010.13042950493000718No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15360.12513411591036291No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14650.11934992175044379No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14510.11820937642313581No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14350.11690589604906952No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14080.11470627291783268No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13810.11250664978659582No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG13670.11136610445928782No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCG13350.10875914371115526No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG12410.10110119651351587No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12390.10093826146675758No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACG350.00206842622.8537084
CTAGCGG4150.019.27578429
TCTAGCG4150.019.27499828
AAGACGG3850.018.6969685
TAGCGGC4450.018.69458230
TAGGACG4600.018.4320134
AGCGGCG4800.018.33132631
CAAGACG4200.017.9020734
CGAACGA1700.017.8825816
CGCAAGA3500.017.8360652
CGGAGTG450.008862426517.7736595
CGGTCCA5000.017.60022410
ACGAACG1750.017.37235815
ATCGTTT2650.016.904529
AACGAAT1051.19074684E-716.7600731
CCGGTCC5700.016.560949
GTCCTAT4550.016.5519621
TAACGAA1850.016.43331113
GCACACG4200.016.3784927
GACGTGA2850.016.2782557
Produced by FastQC (version 0.11.5)