FastQCFastQC Report
Thu 2 Feb 2017
SRR4062977_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062977_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1227483
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT28690.23373032457475992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT21420.17450343507812327No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC18820.15332187899954622No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT16720.13621369908992628No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16510.1345028810989643No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG15770.12847428436890776No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA15750.12831134932214946No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC13710.11169197455280439No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG13490.10989968903846327No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG13440.10949235142156755No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC13290.1082703385708804No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13230.10778153343060555No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT13130.10696685819681415No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC12660.10313788459799444No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA12650.1030564170746153No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA12540.10216027431744473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACG308.41863E-426.66588632
GCGAAGT400.0044844119.99941430
CTAGCGG4400.019.27216329
TCTAGCG4350.019.12587728
CGCCGGT4700.018.7251457
TAGCGGC4650.018.58010130
CTATTAG701.786618E-518.2866691
CGGTCCA4950.017.77870610
AAGACGG3500.017.3730435
GCGCAAG3450.017.1603161
CAAGACG3750.017.0668624
CGCAAGA3400.016.940682
TATTAGG1151.8417268E-816.6951642
ACGAACG1354.4383341E-1016.59210815
TAGGACG4500.016.3557434
AGCGGCG5300.016.30140931
TCTTGCG5650.016.1411192
GCCGGTC5500.016.0014888
TACCGTC3000.016.0014887
CGAACGA1304.802132E-915.99953216
Produced by FastQC (version 0.11.5)