##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062977_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1227483
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.180075813677256	32.0	32.0	32.0	32.0	32.0
2	31.23619960520838	32.0	32.0	32.0	32.0	32.0
3	31.345775053503797	32.0	32.0	32.0	32.0	32.0
4	31.444346683416388	32.0	32.0	32.0	32.0	32.0
5	31.37629034373592	32.0	32.0	32.0	32.0	32.0
6	34.89472196356284	36.0	36.0	36.0	36.0	36.0
7	34.917492136347306	36.0	36.0	36.0	36.0	36.0
8	34.84272042871469	36.0	36.0	36.0	32.0	36.0
9	34.97796222025071	36.0	36.0	36.0	36.0	36.0
10	34.80457081686671	36.0	36.0	36.0	32.0	36.0
11	34.98468329092949	36.0	36.0	36.0	36.0	36.0
12	34.88018326934059	36.0	36.0	36.0	32.0	36.0
13	34.94154379327453	36.0	36.0	36.0	36.0	36.0
14	34.875484222592085	36.0	36.0	36.0	32.0	36.0
15	34.84205972710009	36.0	36.0	36.0	32.0	36.0
16	34.867383906742496	36.0	36.0	36.0	32.0	36.0
17	34.828214321501804	36.0	36.0	36.0	32.0	36.0
18	34.82531326299427	36.0	36.0	36.0	32.0	36.0
19	34.82695890696653	36.0	36.0	36.0	32.0	36.0
20	34.805666555056156	36.0	36.0	36.0	32.0	36.0
21	34.80791913207759	36.0	36.0	36.0	32.0	36.0
22	34.78970869657665	36.0	36.0	36.0	32.0	36.0
23	34.7181859137764	36.0	36.0	36.0	32.0	36.0
24	34.70562525102181	36.0	36.0	36.0	32.0	36.0
25	34.68181799666472	36.0	36.0	36.0	32.0	36.0
26	34.61680121028153	36.0	36.0	36.0	32.0	36.0
27	34.600080001107955	36.0	36.0	36.0	32.0	36.0
28	34.57130730120091	36.0	36.0	36.0	32.0	36.0
29	34.52506389090521	36.0	36.0	36.0	32.0	36.0
30	34.50348884668871	36.0	36.0	36.0	32.0	36.0
31	34.511586718512596	36.0	36.0	36.0	32.0	36.0
32	34.47166192933018	36.0	36.0	36.0	32.0	36.0
33	34.43134691071078	36.0	36.0	36.0	32.0	36.0
34	34.42420954098753	36.0	36.0	36.0	32.0	36.0
35	34.3892941898177	36.0	36.0	36.0	32.0	36.0
36	34.3590713679945	36.0	36.0	36.0	32.0	36.0
37	34.34361127608285	36.0	36.0	36.0	32.0	36.0
38	33.918018416548335	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	7.0
21	26.0
22	74.0
23	255.0
24	839.0
25	2053.0
26	4353.0
27	8335.0
28	14825.0
29	23405.0
30	35502.0
31	50896.0
32	73299.0
33	117111.0
34	283313.0
35	613189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.127941233982085	18.469727288133384	11.888940308430342	26.513391169454188
2	15.663610545816558	20.46759137812204	36.97650963517184	26.892288440889562
3	18.11984361494212	24.39536840836085	28.542880023592993	28.941907953104035
4	11.859184556314203	15.94243160168117	36.29178177386334	35.90660206814128
5	13.974787410324552	37.215046737956236	33.37124291842976	15.43892293328945
6	34.60593751603892	36.00620130787962	16.285602326060726	13.102258850020734
7	30.35584373475022	30.361139149654125	20.8020191009689	18.480998014626753
8	28.026047475622246	33.51123588640198	19.124510876605257	19.338205761370514
9	27.319580489656854	13.965021251703764	18.283960564789385	40.43143769385
10	15.55700931838438	26.77516118572734	31.630122545145856	26.03770695074242
11	38.00208565865263	20.9241260194064	22.065942660681262	19.007845661259708
12	24.914499764558666	23.623971675348397	28.597730964690314	22.86379759540262
13	29.639131164448845	19.242318828300537	25.20639813862851	25.912151868622107
14	23.230953096702763	19.8736764582483	24.946577671544127	31.94879277350481
15	25.005560158470626	27.602989206367827	22.08470504275823	25.306745592403317
16	25.794165784780727	25.720030338505705	23.92807069425809	24.55773318245548
17	23.958946885618783	26.23743057948664	25.02421622132445	24.779406313570128
18	25.1224660545197	24.599200151855463	26.42456148068853	23.853772312936307
19	25.58650506768729	24.982586316877708	25.283120010623367	24.147788604811634
20	25.705121781727325	24.295896562314915	25.190572904064656	24.8084087518931
21	26.727316571648302	24.389767018986834	24.308055026468818	24.574861382896042
22	25.75752638327894	24.621216441463094	25.00558052989779	24.61567664536018
23	23.949698610406188	24.37381922815913	25.764227715133675	25.912254446301002
24	24.762461068707264	25.140877714803384	25.313588864367166	24.783072352122186
25	24.84767560178219	24.638955126727218	25.407440779027585	25.105928492463008
26	24.335978583817454	25.64247325624876	25.823575560720595	24.197972599213184
27	25.261043162449216	24.883439961090982	24.830648833910804	25.024868042549
28	24.235284724920835	24.90934701335986	25.759705022391348	25.09566323932796
29	24.257199488709823	24.996109925758645	25.759460619821212	24.98722996571032
30	24.19585444360533	25.21330234308744	26.080361194411655	24.510482018895576
31	24.99358443253389	24.956842579489898	24.862584654940232	25.186988333035977
32	24.706329945099036	25.004093743049804	24.73818374674028	25.55139256511088
33	24.113816647562533	24.72979259183223	25.566464056936024	25.589926703669214
34	24.973543421782622	24.987718770850595	25.69273871817369	24.34599908919309
35	25.684836368405918	24.69720558248057	25.586912405304187	24.031045643809325
36	24.178746263695707	25.522634529358047	25.39440464755927	24.904214559386972
37	25.453061264392257	25.179574788408477	24.885884366626666	24.4814795805726
38	24.43931922367841	25.04780114722753	25.38597338777545	25.126906241318604
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	53.5
2	66.0
3	66.0
4	261.0
5	456.0
6	456.0
7	635.0
8	814.0
9	826.5
10	839.0
11	839.0
12	1091.5
13	1344.0
14	1854.0
15	2364.0
16	2364.0
17	3699.5
18	5035.0
19	5035.0
20	6034.0
21	7033.0
22	8082.0
23	9131.0
24	9131.0
25	10894.5
26	12658.0
27	12658.0
28	16444.0
29	20230.0
30	24976.0
31	29722.0
32	29722.0
33	38102.5
34	46483.0
35	46483.0
36	51618.0
37	56753.0
38	63767.5
39	70782.0
40	70782.0
41	74042.0
42	77302.0
43	86384.0
44	95466.0
45	95466.0
46	98165.5
47	100865.0
48	100865.0
49	107255.5
50	113646.0
51	113735.0
52	113824.0
53	113824.0
54	108637.5
55	103451.0
56	103451.0
57	99902.5
58	96354.0
59	86075.0
60	75796.0
61	75796.0
62	71646.0
63	67496.0
64	56253.5
65	45011.0
66	45011.0
67	37661.0
68	30311.0
69	30311.0
70	24202.0
71	18093.0
72	14071.5
73	10050.0
74	10050.0
75	7531.0
76	5012.0
77	5012.0
78	4886.5
79	4761.0
80	3719.5
81	2678.0
82	2678.0
83	2533.0
84	2388.0
85	2388.0
86	1505.5
87	623.0
88	538.5
89	454.0
90	454.0
91	271.5
92	89.0
93	60.0
94	31.0
95	31.0
96	21.5
97	12.0
98	12.0
99	15.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008717025001568249
2	8.146752337914251E-5
3	0.0
4	3.2587009351657005E-4
5	8.146752337914251E-5
6	0.0
7	4.888051402748551E-4
8	0.0026069607481325604
9	0.004236311215715411
10	0.001384947897445423
11	0.004317778739094554
12	8.146752337914251E-5
13	8.146752337914251E-5
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	8.146752337914251E-5
22	8.146752337914251E-5
23	1.6293504675828503E-4
24	0.0
25	3.2587009351657005E-4
26	0.0
27	3.2587009351657005E-4
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	1.6293504675828503E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1227483.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.93661861844657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76136137337504	41.941848916469326
2	13.677151682936964	14.753986282184318
3	4.116954724393147	6.66163850517015
4	1.636274192190303	3.530203882375005
5	0.8215434636270101	2.215563823806384
6	0.47632423056274575	1.54147910175527
7	0.2923807138707272	1.1039018938804197
8	0.1965063531779924	0.8479110581970515
9	0.13585605804617326	0.659485475085779
>10	0.7079838267413335	6.994746243986455
>50	0.07853281963161002	2.97963213538699
>100	0.08455700897604586	9.815451149551349
>500	0.010474740838399502	3.883323459223968
>1k	0.004098811632417197	3.0708280729275668
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	2869	0.23373032457475992	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2142	0.17450343507812327	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1882	0.15332187899954622	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1672	0.13621369908992628	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1651	0.1345028810989643	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1577	0.12847428436890776	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1575	0.12831134932214946	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1371	0.11169197455280439	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1349	0.10989968903846327	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG	1344	0.10949235142156755	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGC	1329	0.1082703385708804	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1323	0.10778153343060555	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGT	1313	0.10696685819681415	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCAC	1266	0.10313788459799444	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1265	0.1030564170746153	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	1254	0.10216027431744473	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.146752337914253E-5
2	0.0	0.0	0.0	0.0	8.146752337914253E-5
3	0.0	0.0	0.0	0.0	8.146752337914253E-5
4	0.0	0.0	0.0	0.0	8.146752337914253E-5
5	0.0	0.0	0.0	0.0	8.146752337914253E-5
6	0.0	0.0	0.0	0.0	8.146752337914253E-5
7	0.0	0.0	0.0	0.0	8.146752337914253E-5
8	0.0	0.0	0.0	0.0	8.146752337914253E-5
9	0.0	0.0	0.0	0.0	8.146752337914253E-5
10	0.0	0.0	0.0	0.0	8.146752337914253E-5
11	0.0	0.0	0.0	0.0	8.146752337914253E-5
12	0.0	0.0	0.0	0.0	1.6293504675828505E-4
13	0.0	0.0	0.0	0.0	2.4440257013742757E-4
14	0.0	0.0	0.0	0.0	2.4440257013742757E-4
15	0.0	0.0	0.0	0.0	2.4440257013742757E-4
16	0.0	0.0	0.0	0.0	2.4440257013742757E-4
17	0.0	0.0	0.0	0.0	2.4440257013742757E-4
18	0.0	0.0	0.0	8.146752337914253E-5	2.4440257013742757E-4
19	0.0	0.0	0.0	2.4440257013742757E-4	2.4440257013742757E-4
20	0.0	0.0	0.0	4.888051402748551E-4	2.4440257013742757E-4
21	0.0	0.0	0.0	7.332077104122826E-4	4.073376168957126E-4
22	0.0	0.0	0.0	8.961427571705677E-4	4.073376168957126E-4
23	0.0	0.0	0.0	0.0012220128506871379	4.073376168957126E-4
24	0.0	0.0	0.0	0.0021181556078577056	4.073376168957126E-4
25	0.0	0.0	0.0	0.0025254932247534183	4.073376168957126E-4
26	0.0	0.0	0.0	0.003747506075440556	4.073376168957126E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTACG	30	8.41863E-4	26.665886	32
GCGAAGT	40	0.00448441	19.999414	30
CTAGCGG	440	0.0	19.272163	29
TCTAGCG	435	0.0	19.125877	28
CGCCGGT	470	0.0	18.725145	7
TAGCGGC	465	0.0	18.580101	30
CTATTAG	70	1.786618E-5	18.286669	1
CGGTCCA	495	0.0	17.778706	10
AAGACGG	350	0.0	17.373043	5
GCGCAAG	345	0.0	17.160316	1
CAAGACG	375	0.0	17.066862	4
CGCAAGA	340	0.0	16.94068	2
TATTAGG	115	1.8417268E-8	16.695164	2
ACGAACG	135	4.4383341E-10	16.592108	15
TAGGACG	450	0.0	16.355743	4
AGCGGCG	530	0.0	16.301409	31
TCTTGCG	565	0.0	16.141119	2
GCCGGTC	550	0.0	16.001488	8
TACCGTC	300	0.0	16.001488	7
CGAACGA	130	4.802132E-9	15.999532	16
>>END_MODULE