FastQCFastQC Report
Thu 2 Feb 2017
SRR4062976_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062976_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030245
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT17540.1702507655945916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT11480.1114298055316939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.024.3771081
TCGACTC401.5939363E-423.99879824
ACGCAAT502.795178E-522.40214311
GTATCAA9900.020.864661
CGTAAGA555.8347836E-520.36261729
ACGTAAG555.8369642E-520.3616328
CCGATAA751.5068745E-619.2018369
ATAGCGT507.2070764E-419.1953126
AACGTAA601.1388976E-418.66482527
TAAGACC701.7914768E-518.2803614
TACGGAC450.00884479717.77947813
GTACGGA450.00884479717.77947812
GTATAGG753.2117165E-517.0799081
AACGAAC753.230513E-517.06829814
TCGTTTA854.9852915E-616.94032930
ATCGTTT854.9852915E-616.94032929
CGAACGA703.6932435E-416.0015316
GGTCGTG600.00244249115.9991997
TACGGGT600.00244645115.9953164
TAGCGCG1357.910785E-915.40663729
Produced by FastQC (version 0.11.5)