##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062976_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1030245
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.358350683575267	32.0	32.0	32.0	32.0	32.0
2	31.072812777543206	32.0	32.0	32.0	32.0	32.0
3	31.093369052992248	32.0	32.0	32.0	32.0	32.0
4	31.157116996442593	32.0	32.0	32.0	32.0	32.0
5	31.103575363141776	32.0	32.0	32.0	32.0	32.0
6	34.74929749719727	36.0	36.0	36.0	32.0	36.0
7	34.71438832510714	36.0	36.0	36.0	32.0	36.0
8	34.717035268309964	36.0	36.0	36.0	32.0	36.0
9	34.814619823440054	36.0	36.0	36.0	32.0	36.0
10	34.61707554999054	36.0	36.0	36.0	32.0	36.0
11	34.7829128022946	36.0	36.0	36.0	32.0	36.0
12	34.65775713543866	36.0	36.0	36.0	32.0	36.0
13	34.69840863095671	36.0	36.0	36.0	32.0	36.0
14	34.63771627137234	36.0	36.0	36.0	32.0	36.0
15	34.592575552417145	36.0	36.0	36.0	32.0	36.0
16	34.604906599886434	36.0	36.0	36.0	32.0	36.0
17	34.560011453586284	36.0	36.0	36.0	32.0	36.0
18	34.57457983295236	36.0	36.0	36.0	32.0	36.0
19	34.56390664356537	36.0	36.0	36.0	32.0	36.0
20	34.55210556712238	36.0	36.0	36.0	32.0	36.0
21	34.549829409509385	36.0	36.0	36.0	32.0	36.0
22	34.51602774097424	36.0	36.0	36.0	32.0	36.0
23	34.4717926318497	36.0	36.0	36.0	32.0	36.0
24	34.45858800576562	36.0	36.0	36.0	32.0	36.0
25	34.44358380773505	36.0	36.0	36.0	32.0	36.0
26	34.403977694626036	36.0	36.0	36.0	32.0	36.0
27	34.411380788064974	36.0	36.0	36.0	32.0	36.0
28	34.397887881038	36.0	36.0	36.0	32.0	36.0
29	34.36787463176235	36.0	36.0	36.0	32.0	36.0
30	34.347673611616656	36.0	36.0	36.0	32.0	36.0
31	34.34886070788987	36.0	36.0	36.0	32.0	36.0
32	34.32045581390834	36.0	36.0	36.0	32.0	36.0
33	34.29948216201001	36.0	36.0	36.0	32.0	36.0
34	34.295167654295824	36.0	36.0	36.0	32.0	36.0
35	34.27326509713709	36.0	36.0	36.0	32.0	36.0
36	34.23581866449243	36.0	36.0	36.0	32.0	36.0
37	34.233534741736186	36.0	36.0	36.0	32.0	36.0
38	33.701720464549695	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	4.0
10	6.0
11	6.0
12	11.0
13	8.0
14	65.0
15	154.0
16	257.0
17	288.0
18	373.0
19	441.0
20	567.0
21	798.0
22	1279.0
23	1954.0
24	3062.0
25	4889.0
26	7431.0
27	11187.0
28	16001.0
29	21999.0
30	30716.0
31	42160.0
32	58531.0
33	89286.0
34	213290.0
35	525476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.846482383981105	17.16132104350192	11.426141967960273	26.566054604556694
2	16.600855001330476	19.53839251203742	37.633946000120424	26.226806486511684
3	19.203390603810547	22.900069019677886	28.62171294997481	29.274827426536753
4	13.355328678007956	15.417222374293596	35.573635871242786	35.65381307645566
5	15.019354638318688	35.66504633853727	34.16734288188041	15.148256141263621
6	34.466173966893216	35.10553831041639	16.743444744050425	13.684842978639969
7	30.335502720226742	30.044989298661967	20.854262820979475	18.765245160131812
8	27.709271626028887	33.50452321789098	19.54437800900761	19.241827147072527
9	27.2985376251405	14.514065627299216	18.486142090887384	39.701254656672894
10	15.890937504549932	26.98889088411867	32.26370682417119	24.856464787160213
11	36.7226933171815	21.455333985618662	22.342907950115702	19.47906474708413
12	24.309685610535237	23.530822087342596	29.20534371818031	22.954148583941862
13	29.910434314938033	19.760465671664974	24.69434472207067	25.63475529132632
14	23.634258000885318	19.1351433208819	25.552936699620243	31.67766197861253
15	25.313373482161666	26.74628471234494	22.24269685175063	25.697644953742767
16	25.31821411791202	26.05987240371062	23.69607930695722	24.92583417142014
17	24.1737352516002	25.834468416847322	25.328365178776867	24.663431152775605
18	24.788217239073056	25.355883372177562	25.79939680619851	24.056502582550866
19	25.704578437225468	25.08690439788187	25.275905082244904	23.932612082647754
20	25.71278252124855	24.632105984538743	25.193945182820997	24.461166311391707
21	26.126101639633887	24.663817295997063	24.7607916759289	24.44928938844015
22	25.968900067270905	24.46467209558099	24.95012925139031	24.616298585757786
23	25.005654598669107	24.667349425075354	25.177767962451526	25.149228013804013
24	24.846615046481464	24.877583274438688	25.381520802106227	24.894280876973617
25	25.22937605635307	24.344490751662253	25.402566992564633	25.023566199420046
26	24.881413523615358	25.06150186884132	25.370321829037422	24.686762778505898
27	25.08144492420477	25.15803615805169	24.948453808399602	24.812065109343937
28	25.028926983633927	24.79324001630783	25.307616144751403	24.87021685530684
29	24.79655342129047	25.19563197792885	25.25950784517965	24.74830675560103
30	24.803364760674313	25.09068565831915	25.332967063900753	24.772982517105785
31	25.228383083683674	24.801466155815945	24.86582402829805	25.10432673220233
32	25.037857902788673	24.94758136536953	24.73363567884073	25.280925053001063
33	24.559528455189845	24.89734464823433	25.177110817301624	25.3660160792742
34	25.171439000333944	24.660325559360995	25.31317226221819	24.855063178086873
35	25.30070771889289	24.741910743930024	25.464968400205812	24.492413136971276
36	24.961799306077356	25.109068563207344	25.068683585642365	24.860448545072934
37	25.33879153351221	24.735664943910102	24.973013167244588	24.952530355333103
38	24.916761957642272	24.867450091149294	25.24156019639324	24.974227754815185
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	38.0
2	65.0
3	65.0
4	174.0
5	283.0
6	283.0
7	344.5
8	406.0
9	387.5
10	369.0
11	369.0
12	545.5
13	722.0
14	1084.0
15	1446.0
16	1446.0
17	2334.0
18	3222.0
19	3222.0
20	4329.5
21	5437.0
22	6312.0
23	7187.0
24	7187.0
25	8682.5
26	10178.0
27	10178.0
28	13176.0
29	16174.0
30	20651.0
31	25128.0
32	25128.0
33	30351.5
34	35575.0
35	35575.0
36	40223.5
37	44872.0
38	50468.0
39	56064.0
40	56064.0
41	61287.0
42	66510.0
43	72820.5
44	79131.0
45	79131.0
46	83613.5
47	88096.0
48	88096.0
49	91902.5
50	95709.0
51	95978.0
52	96247.0
53	96247.0
54	95312.0
55	94377.0
56	94377.0
57	89752.5
58	85128.0
59	77430.5
60	69733.0
61	69733.0
62	62128.5
63	54524.0
64	45431.5
65	36339.0
66	36339.0
67	30127.0
68	23915.0
69	23915.0
70	19114.0
71	14313.0
72	11144.5
73	7976.0
74	7976.0
75	6137.0
76	4298.0
77	4298.0
78	3549.5
79	2801.0
80	2184.5
81	1568.0
82	1568.0
83	1314.5
84	1061.0
85	1061.0
86	732.5
87	404.0
88	308.0
89	212.0
90	212.0
91	163.0
92	114.0
93	107.0
94	100.0
95	100.0
96	85.5
97	71.0
98	71.0
99	275.0
100	479.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11744779154472965
2	0.052317652597197756
3	0.01009468621541478
4	0.0026207358443865294
5	9.706429053283442E-5
6	2.9119287159850324E-4
7	0.0
8	4.8532145266417213E-4
9	2.9119287159850324E-4
10	9.706429053283443E-4
11	0.002038350101189523
12	0.002523671553853695
13	0.015821479356852013
14	0.010580007668078952
15	0.023683686890011598
16	0.011744779154472966
17	0.021160015336157904
18	0.006017986013035734
19	0.008735786147955098
20	0.005532664560371562
21	0.007376886080495416
22	0.007959271823692424
23	0.010677071958611787
24	0.015239093613655006
25	0.016306800809516183
26	0.018927536653902715
27	0.009803493343816277
28	0.006309178884634237
29	0.011841843445005799
30	0.003688443040247708
31	0.006600371756232741
32	0.00747395037102825
33	0.009609364762750608
34	0.013686064965129653
35	0.016986250843246023
36	0.015433222194720674
37	0.01096826483021029
38	0.006503307465699906
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1030245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.14387356408479
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.04153476584197	22.7197049833955
2	16.06865566669243	12.579788515236462
3	8.257151477455722	9.69650680299068
4	5.024523985430559	7.867173264216208
5	3.3129768934136994	6.484137431826014
6	2.256945494722187	5.300735345186166
7	1.5737800467335892	4.312269301691336
8	1.1664077340575656	3.652617349289599
9	0.8518767532657215	3.0011180329814815
>10	3.351368994403693	19.97067146050809
>50	0.059955843411171786	1.6158977413598514
>100	0.034076151519288916	2.4479968667575847
>500	2.4873101746353856E-4	0.06883560917834655
>1k	4.974620349270771E-4	0.28254729538268986
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1754	0.1702507655945916	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1148	0.1114298055316939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8532145266417213E-4
2	0.0	0.0	0.0	0.0	4.8532145266417213E-4
3	0.0	0.0	0.0	0.0	4.8532145266417213E-4
4	0.0	0.0	0.0	0.0	4.8532145266417213E-4
5	0.0	0.0	0.0	0.0	4.8532145266417213E-4
6	0.0	0.0	0.0	0.0	4.8532145266417213E-4
7	0.0	0.0	0.0	0.0	4.8532145266417213E-4
8	0.0	0.0	0.0	0.0	4.8532145266417213E-4
9	0.0	0.0	0.0	0.0	4.8532145266417213E-4
10	0.0	0.0	0.0	0.0	4.8532145266417213E-4
11	0.0	0.0	0.0	0.0	4.8532145266417213E-4
12	0.0	0.0	0.0	0.0	0.0017471572295910196
13	0.0	0.0	0.0	0.0	0.0026207358443865294
14	0.0	0.0	0.0	0.0	0.0026207358443865294
15	0.0	0.0	0.0	0.0	0.0031060572970507015
16	0.0	0.0	0.0	0.0	0.0031060572970507015
17	0.0	0.0	0.0	9.706429053283442E-5	0.0031060572970507015
18	0.0	0.0	0.0	9.706429053283442E-5	0.003397250168649205
19	0.0	0.0	0.0	9.706429053283442E-5	0.003397250168649205
20	0.0	0.0	0.0	9.706429053283442E-5	0.003979635911846211
21	0.0	0.0	0.0	1.9412858106566884E-4	0.0041737644929118805
22	0.0	0.0	0.0	2.911928715985033E-4	0.004367893073977549
23	0.0	0.0	0.0	3.882571621313377E-4	0.004367893073977549
24	0.0	0.0	0.0	4.8532145266417213E-4	0.004367893073977549
25	0.0	0.0	0.0	6.794500337298409E-4	0.004367893073977549
26	0.0	0.0	0.0	8.735786147955098E-4	0.004562021655043218
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	335	0.0	24.377108	1
TCGACTC	40	1.5939363E-4	23.998798	24
ACGCAAT	50	2.795178E-5	22.402143	11
GTATCAA	990	0.0	20.86466	1
CGTAAGA	55	5.8347836E-5	20.362617	29
ACGTAAG	55	5.8369642E-5	20.36163	28
CCGATAA	75	1.5068745E-6	19.201836	9
ATAGCGT	50	7.2070764E-4	19.195312	6
AACGTAA	60	1.1388976E-4	18.664825	27
TAAGACC	70	1.7914768E-5	18.280361	4
TACGGAC	45	0.008844797	17.779478	13
GTACGGA	45	0.008844797	17.779478	12
GTATAGG	75	3.2117165E-5	17.079908	1
AACGAAC	75	3.230513E-5	17.068298	14
TCGTTTA	85	4.9852915E-6	16.940329	30
ATCGTTT	85	4.9852915E-6	16.940329	29
CGAACGA	70	3.6932435E-4	16.00153	16
GGTCGTG	60	0.002442491	15.999199	7
TACGGGT	60	0.002446451	15.995316	4
TAGCGCG	135	7.910785E-9	15.406637	29
>>END_MODULE