FastQCFastQC Report
Thu 2 Feb 2017
SRR4062976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030245
Sequences flagged as poor quality0
Sequence length38
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT17380.1686977369460662No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT12310.11948614164591917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3350.025.3173891
GTACGAG350.002064519622.8607161
GTATCAA9700.020.4568041
GTATAAT753.2288335E-517.0693341
TAAGACC805.6217737E-515.9993934
CAATACT1451.2205419E-915.4476914
CTATTAG650.004156869414.7715391
ATTATAC1103.5427493E-614.5449043
ATACCGT1553.1250238E-914.4531696
TACCGTC1553.1250238E-914.4531697
CCGTCGT1258.5766806E-714.0815179
TATCAAC14000.014.056612
ACCGTGT1155.606169E-613.9145428
GTCTAGT700.006796125313.7164291
GCGAAAG2450.013.71376618
GTTAGTA700.00680486713.7137662
ATCGTTT1053.683355E-513.71376629
TCAATAC700.00680486713.7137663
TAGGACG700.00680486713.7137664
TATAACC1402.055458E-713.7137664
Produced by FastQC (version 0.11.5)