##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1030245
Sequences flagged as poor quality	0
Sequence length	38
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.266382268295406	32.0	32.0	32.0	32.0	32.0
2	31.347037840513664	32.0	32.0	32.0	32.0	32.0
3	31.389716038418047	32.0	32.0	32.0	32.0	32.0
4	31.47179360249261	32.0	32.0	32.0	32.0	32.0
5	31.412810545064524	32.0	32.0	32.0	32.0	32.0
6	34.925407063368425	36.0	36.0	36.0	36.0	36.0
7	34.963429087255946	36.0	36.0	36.0	36.0	36.0
8	34.89334769884833	36.0	36.0	36.0	36.0	36.0
9	34.99992914306791	36.0	36.0	36.0	36.0	36.0
10	34.8628840712646	36.0	36.0	36.0	32.0	36.0
11	35.015323539546415	36.0	36.0	36.0	36.0	36.0
12	34.93784099898568	36.0	36.0	36.0	36.0	36.0
13	34.98140636450553	36.0	36.0	36.0	36.0	36.0
14	34.925099369567434	36.0	36.0	36.0	32.0	36.0
15	34.897933015933106	36.0	36.0	36.0	32.0	36.0
16	34.922933865245646	36.0	36.0	36.0	32.0	36.0
17	34.90014705240016	36.0	36.0	36.0	32.0	36.0
18	34.8891341379963	36.0	36.0	36.0	32.0	36.0
19	34.87679047216924	36.0	36.0	36.0	32.0	36.0
20	34.87933743915282	36.0	36.0	36.0	32.0	36.0
21	34.86815757416925	36.0	36.0	36.0	32.0	36.0
22	34.84591335070784	36.0	36.0	36.0	32.0	36.0
23	34.80193837388194	36.0	36.0	36.0	32.0	36.0
24	34.77854685050643	36.0	36.0	36.0	32.0	36.0
25	34.7752262811273	36.0	36.0	36.0	32.0	36.0
26	34.71003207974802	36.0	36.0	36.0	32.0	36.0
27	34.695763629039696	36.0	36.0	36.0	32.0	36.0
28	34.670437614353865	36.0	36.0	36.0	32.0	36.0
29	34.643984683254956	36.0	36.0	36.0	32.0	36.0
30	34.63229037753156	36.0	36.0	36.0	32.0	36.0
31	34.61985450062849	36.0	36.0	36.0	32.0	36.0
32	34.597209401647184	36.0	36.0	36.0	32.0	36.0
33	34.58300598401351	36.0	36.0	36.0	32.0	36.0
34	34.57044004096113	36.0	36.0	36.0	32.0	36.0
35	34.555007789409316	36.0	36.0	36.0	32.0	36.0
36	34.52925372120224	36.0	36.0	36.0	32.0	36.0
37	34.5162034273401	36.0	36.0	36.0	32.0	36.0
38	34.13742168125058	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	17.0
22	41.0
23	197.0
24	642.0
25	1523.0
26	3362.0
27	6772.0
28	11709.0
29	18695.0
30	27719.0
31	40140.0
32	56535.0
33	89162.0
34	221181.0
35	552548.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.276976004395486	18.37863916509003	12.022070636520807	27.322314193993673
2	15.74373086013521	20.42368562817582	36.854728729574035	26.977854782114935
3	18.212852282709452	23.765415022640248	28.85488403243889	29.166848662211414
4	12.80503725820025	16.366624184779706	35.396794736775696	35.43154382024435
5	14.466364796723111	36.35222689748555	33.73119986022742	15.45020844556392
6	34.54427741804603	35.52016368953732	16.39846133387948	13.537097558537162
7	30.16547536311786	30.36610744639134	20.865736660441602	18.60268053004919
8	27.721980363322217	33.76023451415481	19.327130744553322	19.190654377969647
9	27.417826795133383	14.300302270816792	18.31708732850451	39.96478360554532
10	15.583844541077807	27.32440984624942	32.319944867215405	24.77180074545737
11	36.977746025521306	21.414524198260917	22.26261359466821	19.345116181549564
12	24.26274317196609	23.604739875640384	29.143265924961025	22.9892510274325
13	29.8508607175963	19.766851574140134	24.73916398526564	25.643123722997927
14	23.5988527000859	19.11399715601629	25.45248945639144	31.83466068750637
15	25.354842780115412	26.918742629180436	21.99913612781426	25.727278462889895
16	25.334459279103516	26.038660706919224	23.669806696465404	24.957073317511856
17	24.092036360283235	25.877145727472588	25.240306917286663	24.79051099495751
18	24.872530320457756	25.4210406262588	25.67476668171163	24.03166237157181
19	25.627399307931608	25.05928201544293	25.413566675887772	23.89975200073769
20	25.77018087930541	24.615407014836276	25.05967027260506	24.554741833253253
21	26.12480744833986	24.700871541956605	24.716110665153753	24.45821034454978
22	25.789302544540377	24.673839717737042	24.988328019063427	24.548529718659154
23	25.081242890033817	24.635717364041916	25.126183700172	25.156856045752267
24	24.950181752884024	24.888642992686204	25.28815961251935	24.87301564191042
25	25.040888411763053	24.510236905273803	25.405365530268103	25.043509152695044
26	25.01482657037889	25.099175438851923	25.164305577799457	24.72169241296973
27	25.12705727963473	25.31662402304697	24.756562523052793	24.799756174265514
28	24.97745681852375	24.772165844046803	25.355910487311274	24.894466850118178
29	24.914365029677406	25.025115385175372	25.18430082164922	24.876218763498002
30	24.915529801163803	25.008808584365855	25.307281277754324	24.76838033671602
31	25.223611859315014	24.759159229115404	24.919800629947243	25.09742828162233
32	25.145475105436088	24.922033108629503	24.734407835029533	25.19808395090488
33	24.61861013642386	24.82788074681265	25.195172022188896	25.35833709457459
34	25.018903270581273	24.731787099185144	25.38735931744391	24.861950312789677
35	25.216429101815585	24.761585836378725	25.384059131565795	24.637925930239895
36	24.991337012069945	25.067629544428755	25.06927963736781	24.871753806133494
37	25.396871617916126	24.749258671481055	24.942707802512995	24.911161908089824
38	24.931278415598634	24.680852302949592	25.12744553717372	25.260423744278054
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	29.5
2	54.0
3	54.0
4	137.0
5	220.0
6	220.0
7	287.5
8	355.0
9	328.0
10	301.0
11	301.0
12	506.5
13	712.0
14	1033.5
15	1355.0
16	1355.0
17	2296.5
18	3238.0
19	3238.0
20	4340.5
21	5443.0
22	6241.0
23	7039.0
24	7039.0
25	8682.5
26	10326.0
27	10326.0
28	13325.5
29	16325.0
30	20713.5
31	25102.0
32	25102.0
33	30611.5
34	36121.0
35	36121.0
36	40759.0
37	45397.0
38	51071.0
39	56745.0
40	56745.0
41	61784.0
42	66823.0
43	72931.5
44	79040.0
45	79040.0
46	83978.5
47	88917.0
48	88917.0
49	92092.5
50	95268.0
51	96011.0
52	96754.0
53	96754.0
54	95322.0
55	93890.0
56	93890.0
57	89227.0
58	84564.0
59	77380.5
60	70197.0
61	70197.0
62	62294.5
63	54392.0
64	45332.0
65	36272.0
66	36272.0
67	29815.5
68	23359.0
69	23359.0
70	18886.5
71	14414.0
72	11089.0
73	7764.0
74	7764.0
75	5903.5
76	4043.0
77	4043.0
78	3403.5
79	2764.0
80	2189.5
81	1615.0
82	1615.0
83	1256.5
84	898.0
85	898.0
86	625.0
87	352.0
88	238.5
89	125.0
90	125.0
91	80.0
92	35.0
93	25.5
94	16.0
95	16.0
96	9.0
97	2.0
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009512300472217773
2	0.0
3	0.0
4	1.9412858106566884E-4
5	0.0
6	1.9412858106566884E-4
7	9.706429053283442E-5
8	0.002911928715985033
9	0.00417376449291188
10	4.8532145266417213E-4
11	0.004562021655043218
12	6.794500337298409E-4
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.9412858106566884E-4
22	0.0
23	9.706429053283442E-5
24	0.0
25	1.9412858106566884E-4
26	0.0
27	9.706429053283442E-5
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	9.706429053283442E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1030245.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.18898857589713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	54.84641880218237	20.3968284226323
2	16.983764445580963	12.632180438848767
3	9.06418724026422	10.112638671839356
4	5.4339200105193335	8.083279567741693
5	3.5422094728998146	6.586559381055289
6	2.423794715077567	5.408308438160369
7	1.7222536968899138	4.483421114090493
8	1.2744177073276692	3.791544444698377
9	0.9241373371098405	3.0930959585104065
>10	3.6787256540498885	20.799523166800356
>50	0.0679012253063379	1.7313002080681583
>100	0.03748332928004276	2.530586384009898
>500	2.6212117066493827E-4	0.06131505348234918
>1k	5.242423413298765E-4	0.2894187500621538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	1738	0.1686977369460662	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	1231	0.11948614164591917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.8532145266417213E-4
2	0.0	0.0	0.0	0.0	4.8532145266417213E-4
3	0.0	0.0	0.0	0.0	4.8532145266417213E-4
4	0.0	0.0	0.0	0.0	4.8532145266417213E-4
5	0.0	0.0	0.0	0.0	8.735786147955098E-4
6	0.0	0.0	0.0	0.0	0.0011647714863940132
7	0.0	0.0	0.0	0.0	0.0012618357769268475
8	0.0	0.0	0.0	0.0	0.0012618357769268475
9	0.0	0.0	0.0	0.0	0.0012618357769268475
10	0.0	0.0	0.0	0.0	0.0012618357769268475
11	0.0	0.0	0.0	0.0	0.0012618357769268475
12	0.0	0.0	0.0	0.0	0.0024266072633208607
13	0.0	0.0	0.0	0.0	0.0026207358443865294
14	0.0	0.0	0.0	0.0	0.0026207358443865294
15	0.0	0.0	0.0	0.0	0.003397250168649205
16	0.0	0.0	0.0	0.0	0.003882571621313377
17	0.0	0.0	0.0	9.706429053283442E-5	0.003979635911846211
18	0.0	0.0	0.0	9.706429053283442E-5	0.0041737644929118805
19	0.0	0.0	0.0	9.706429053283442E-5	0.0041737644929118805
20	0.0	0.0	0.0	9.706429053283442E-5	0.004659085945576053
21	0.0	0.0	0.0	1.9412858106566884E-4	0.004853214526641721
22	0.0	0.0	0.0	2.911928715985033E-4	0.00504734310770739
23	0.0	0.0	0.0	3.882571621313377E-4	0.00504734310770739
24	0.0	0.0	0.0	3.882571621313377E-4	0.00504734310770739
25	0.0	0.0	0.0	5.823857431970066E-4	0.00504734310770739
26	0.0	0.0	0.0	7.765143242626754E-4	0.0053385359793058935
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	335	0.0	25.317389	1
GTACGAG	35	0.0020645196	22.860716	1
GTATCAA	970	0.0	20.456804	1
GTATAAT	75	3.2288335E-5	17.069334	1
TAAGACC	80	5.6217737E-5	15.999393	4
CAATACT	145	1.2205419E-9	15.447691	4
CTATTAG	65	0.0041568694	14.771539	1
ATTATAC	110	3.5427493E-6	14.544904	3
ATACCGT	155	3.1250238E-9	14.453169	6
TACCGTC	155	3.1250238E-9	14.453169	7
CCGTCGT	125	8.5766806E-7	14.081517	9
TATCAAC	1400	0.0	14.05661	2
ACCGTGT	115	5.606169E-6	13.914542	8
GTCTAGT	70	0.0067961253	13.716429	1
GCGAAAG	245	0.0	13.713766	18
GTTAGTA	70	0.006804867	13.713766	2
ATCGTTT	105	3.683355E-5	13.713766	29
TCAATAC	70	0.006804867	13.713766	3
TAGGACG	70	0.006804867	13.713766	4
TATAACC	140	2.055458E-7	13.713766	4
>>END_MODULE