FastQCFastQC Report
Thu 2 Feb 2017
SRR4062975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344870
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT40420.30054949549027044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT28070.20871905834764698No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG16840.12521656368273515No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG16210.12053209603902237No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16080.1195654598585737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT15340.11406306929294281No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG14850.11041959445894399No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC14840.11034523782967871No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC14800.11004781131261758No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG14710.10937860164923004No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC14390.10699918951274101No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14000.10409928097139501No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13970.10387621108359915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTT3300.017.93914229
CGAGTAT450.00885364217.77686918
CTATTAG753.2039E-517.0854911
GTCCTAA4300.016.390151
CATCGTT3650.016.2189528
TAGCGGC3200.015.99977730
CTAGCGG3250.015.75362629
GACGTGA3600.015.5547597
TCTAGCG3200.015.499783528
CGTGAAA4000.015.1997889
GTCCTAC5500.015.1439561
GGACGTG6800.015.0552546
ATTAGAC756.2495156E-414.9320143
TCGTTTA4200.014.856935530
ACGTATG650.00416245414.76902532
TACCGTC3150.014.7294067
CGAGCCG4350.014.71243915
CGTTTAT4250.014.68214931
TAGGACG7200.014.6637344
TTAGGAC4050.014.6159933
Produced by FastQC (version 0.11.5)