##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062975_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1344870
Sequences flagged as poor quality	0
Sequence length	38
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.202479050019704	32.0	32.0	32.0	32.0	32.0
2	30.788192167272673	32.0	32.0	32.0	32.0	32.0
3	30.84733245592511	32.0	32.0	32.0	32.0	32.0
4	30.916404559548507	32.0	32.0	32.0	32.0	32.0
5	30.822152326990714	32.0	32.0	32.0	32.0	32.0
6	34.46961416345074	36.0	36.0	36.0	32.0	36.0
7	34.38714150810115	36.0	36.0	36.0	32.0	36.0
8	34.36938440146631	36.0	36.0	36.0	32.0	36.0
9	34.51352026589931	36.0	36.0	36.0	32.0	36.0
10	34.23243064385405	36.0	36.0	36.0	32.0	36.0
11	34.52266315703377	36.0	36.0	36.0	32.0	36.0
12	34.321862336136576	36.0	36.0	36.0	32.0	36.0
13	34.41337303977336	36.0	36.0	36.0	32.0	36.0
14	34.31281164722241	36.0	36.0	36.0	32.0	36.0
15	34.26025043312737	36.0	36.0	36.0	32.0	36.0
16	34.263086394967544	36.0	36.0	36.0	32.0	36.0
17	34.19314060095028	36.0	36.0	36.0	32.0	36.0
18	34.21654063218006	36.0	36.0	36.0	32.0	36.0
19	34.197907604452475	36.0	36.0	36.0	32.0	36.0
20	34.18254031988222	36.0	36.0	36.0	32.0	36.0
21	34.153859480842016	36.0	36.0	36.0	32.0	36.0
22	34.130927896376605	36.0	36.0	36.0	32.0	36.0
23	34.09144006483898	36.0	36.0	36.0	32.0	36.0
24	34.07183593953319	36.0	36.0	36.0	32.0	36.0
25	34.05185333898444	36.0	36.0	36.0	32.0	36.0
26	33.99232639585982	36.0	36.0	36.0	32.0	36.0
27	34.00315495177973	36.0	36.0	36.0	32.0	36.0
28	33.97898235517187	36.0	36.0	36.0	32.0	36.0
29	33.94277142028598	36.0	36.0	36.0	32.0	36.0
30	33.91321242945415	36.0	36.0	36.0	32.0	36.0
31	33.93187743053232	36.0	36.0	36.0	32.0	36.0
32	33.87575676459435	36.0	36.0	36.0	32.0	36.0
33	33.846858060630396	36.0	36.0	36.0	32.0	36.0
34	33.84812881542454	36.0	36.0	36.0	32.0	36.0
35	33.80410671663432	36.0	36.0	36.0	32.0	36.0
36	33.75943920230208	36.0	36.0	36.0	32.0	36.0
37	33.75070899046005	36.0	36.0	36.0	32.0	36.0
38	33.14175124733246	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	11.0
11	10.0
12	7.0
13	10.0
14	207.0
15	431.0
16	551.0
17	619.0
18	777.0
19	1021.0
20	1335.0
21	1788.0
22	2830.0
23	4303.0
24	6370.0
25	9574.0
26	13900.0
27	19780.0
28	27624.0
29	37205.0
30	49393.0
31	66459.0
32	89200.0
33	134055.0
34	295784.0
35	581621.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43445794936622	17.33774365067666	11.355971572660797	25.871826827296317
2	16.385200483278677	20.17926544021807	38.10521429124088	25.33031978526238
3	18.70573467426967	24.660155332066168	28.733011484816306	27.90109850884786
4	12.457885168068945	15.645488108206582	36.40833262073042	35.48829410299405
5	14.416953931538263	36.77141548464236	33.71602268772846	15.095607896090918
6	33.40659945318094	35.98595848368875	17.21334940429142	13.394092658838892
7	29.503818212912776	30.789667402797292	21.450846550224185	18.255667834065747
8	27.77201926136989	33.15804971194199	19.868938810351647	19.200992216336473
9	27.904948363000408	14.088063595785167	18.743089475629223	39.2638985655852
10	16.010653841606644	26.536750474210464	31.774814907347714	25.677780776835178
11	37.1639768955302	21.12037940347162	22.831502147454582	18.8841415535436
12	25.071346671207472	23.859828886962188	29.068916645970305	21.999907795860036
13	29.838696483152	19.609718369562792	25.183204801177983	25.368380346107223
14	23.68092876535691	20.181401342848833	25.267168022677772	30.870501869116485
15	24.78580098531547	27.695932598710044	22.535149463312468	24.98311695266202
16	25.269813129356365	25.706853510043302	24.412241295073276	24.611092065527053
17	23.734100254574418	25.932825969416857	25.64337121085351	24.689702565155212
18	24.788518738845923	24.58120166567519	26.998661511005356	23.631618084473526
19	25.66820026339096	24.792362108287744	25.701886346279544	23.83755128204175
20	25.896850732717176	24.198294304905826	25.386659171066984	24.518195791310013
21	27.03863139664497	24.15832395762303	24.403195450283206	24.399849195448798
22	25.93952869263889	24.632911204146435	24.96471515575154	24.462844947463136
23	24.039478322171306	24.435986787017246	25.77780950638712	25.746725384424323
24	24.851527117774356	25.068975005391575	25.655429876030905	24.42406800080316
25	24.733406660910525	24.617391427217918	25.71358664813398	24.935615263737578
26	24.310746560147315	25.523071053475103	26.13774879946334	24.028433586914243
27	25.018553744881917	25.05781778214706	25.198588279387423	24.725040193583602
28	24.082255160839118	25.07460267060086	25.883724682868937	24.959417485691084
29	24.282682348601803	25.168881234327735	25.66838596835652	24.880050448713945
30	24.12924391580541	25.28061021521567	26.11089671090251	24.479249158076406
31	25.062091017251635	24.880948839976206	24.807183224271267	25.249776918500892
32	24.370213439957883	25.377068831281825	24.881226013752425	25.37149171500787
33	23.973806303174342	24.801320095690578	25.92606363557268	25.2988099655624
34	24.90966299220562	24.962463383672183	25.90796222466641	24.219911399455786
35	25.506230645434773	24.988993369248664	25.71319992503592	23.79157606028064
36	24.15745628321427	25.593710910716172	25.396563340814676	24.852269465254878
37	25.136588859167908	25.487217127019647	24.974325681977557	24.40186833183489
38	24.32011386215184	25.53696355697272	25.436352603838237	24.706569977037205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	845.0
1	623.5
2	402.0
3	402.0
4	811.5
5	1221.0
6	1221.0
7	1432.0
8	1643.0
9	1540.0
10	1437.0
11	1437.0
12	1849.0
13	2261.0
14	2872.5
15	3484.0
16	3484.0
17	5139.5
18	6795.0
19	6795.0
20	8038.5
21	9282.0
22	9893.5
23	10505.0
24	10505.0
25	12690.5
26	14876.0
27	14876.0
28	19128.0
29	23380.0
30	29423.0
31	35466.0
32	35466.0
33	44009.5
34	52553.0
35	52553.0
36	57740.5
37	62928.0
38	70566.5
39	78205.0
40	78205.0
41	81524.5
42	84844.0
43	93742.5
44	102641.0
45	102641.0
46	105408.0
47	108175.0
48	108175.0
49	113925.5
50	119676.0
51	120586.5
52	121497.0
53	121497.0
54	115692.0
55	109887.0
56	109887.0
57	107295.0
58	104703.0
59	94191.5
60	83680.0
61	83680.0
62	78913.0
63	74146.0
64	61052.0
65	47958.0
66	47958.0
67	40066.5
68	32175.0
69	32175.0
70	25710.0
71	19245.0
72	15083.0
73	10921.0
74	10921.0
75	8162.0
76	5403.0
77	5403.0
78	5309.0
79	5215.0
80	4072.5
81	2930.0
82	2930.0
83	3052.0
84	3174.0
85	3174.0
86	2125.0
87	1076.0
88	904.0
89	732.0
90	732.0
91	536.0
92	340.0
93	308.0
94	276.0
95	276.0
96	248.5
97	221.0
98	221.0
99	446.5
100	672.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.12313457806330723
2	0.05338805981247258
3	0.010261214838608935
4	0.002602482024284875
5	1.487132585305643E-4
6	7.435662926528215E-5
7	0.0
8	1.487132585305643E-4
9	7.435662926528215E-5
10	2.2306988779584644E-4
11	0.0016358458438362072
12	0.0020819856194279
13	0.01487132585305643
14	0.00869972562403801
15	0.02424026114048198
16	0.010632997984935347
17	0.020299359789422027
18	0.00520496404856975
19	0.007510019555793497
20	0.006022886970487853
21	0.006171600229018419
22	0.007435662926528215
23	0.009815075063017242
24	0.013384193267750786
25	0.016284101809096792
26	0.019555793496769204
27	0.009517648545956115
28	0.00587417371195729
29	0.010261214838608935
30	0.0024537687657543105
31	0.00520496404856975
32	0.0066177400046101105
33	0.008848438882568576
34	0.013607263155546634
35	0.016804598213953765
36	0.014276472818934172
37	0.010484284726404784
38	0.006394670116814265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
38	1344870.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.60742485816513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.09648262274943	45.90663046328783
2	11.870518266187652	13.439189415613884
3	3.2399732709981794	5.502196304414789
4	1.304766897753877	2.954379764880953
5	0.6613639074910969	1.8719053848602372
6	0.40410672398400443	1.3725264607562284
7	0.2588116197635121	1.025546152272811
8	0.18128068064631914	0.8209466006338832
9	0.1313037341322774	0.6689489637140446
>10	0.6860733246527704	7.122161210230265
>50	0.07195807084633526	2.892850688061921
>100	0.08030620344729064	9.812251516043197
>500	0.00949431079802616	3.671668900566967
>1k	0.003560366549259809	2.9387981746629563
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4042	0.30054949549027044	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	2807	0.20871905834764698	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG	1684	0.12521656368273515	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG	1621	0.12053209603902237	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA	1608	0.1195654598585737	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	1534	0.11406306929294281	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG	1485	0.11041959445894399	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC	1484	0.11034523782967871	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC	1480	0.11004781131261758	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGG	1471	0.10937860164923004	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC	1439	0.10699918951274101	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	1400	0.10409928097139501	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT	1397	0.10387621108359915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.435662926528215E-5
2	0.0	0.0	0.0	0.0	7.435662926528215E-5
3	0.0	0.0	0.0	0.0	7.435662926528215E-5
4	0.0	0.0	0.0	0.0	7.435662926528215E-5
5	0.0	0.0	0.0	0.0	7.435662926528215E-5
6	0.0	0.0	0.0	0.0	7.435662926528215E-5
7	0.0	0.0	0.0	0.0	7.435662926528215E-5
8	0.0	0.0	0.0	0.0	7.435662926528215E-5
9	0.0	0.0	0.0	0.0	7.435662926528215E-5
10	0.0	0.0	0.0	0.0	7.435662926528215E-5
11	0.0	0.0	0.0	0.0	2.2306988779584644E-4
12	0.0	0.0	0.0	0.0	2.974265170611286E-4
13	0.0	0.0	0.0	0.0	3.717831463264107E-4
14	0.0	0.0	0.0	0.0	3.717831463264107E-4
15	0.0	0.0	0.0	0.0	5.20496404856975E-4
16	0.0	0.0	0.0	7.435662926528215E-5	5.20496404856975E-4
17	0.0	0.0	0.0	1.487132585305643E-4	5.20496404856975E-4
18	0.0	0.0	0.0	1.487132585305643E-4	5.20496404856975E-4
19	0.0	0.0	0.0	1.487132585305643E-4	5.20496404856975E-4
20	0.0	0.0	0.0	2.974265170611286E-4	5.20496404856975E-4
21	0.0	0.0	0.0	5.948530341222572E-4	5.948530341222572E-4
22	0.0	0.0	0.0	0.00104099280971395	6.692096633875393E-4
23	0.0	0.0	0.0	0.0018589157316320536	6.692096633875393E-4
24	0.0	0.0	0.0	0.003048621799876568	6.692096633875393E-4
25	0.0	0.0	0.0	0.003494761575468261	6.692096633875393E-4
26	0.0	0.0	0.0	0.004758824272978058	6.692096633875393E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTTT	330	0.0	17.939142	29
CGAGTAT	45	0.008853642	17.776869	18
CTATTAG	75	3.2039E-5	17.085491	1
GTCCTAA	430	0.0	16.39015	1
CATCGTT	365	0.0	16.21895	28
TAGCGGC	320	0.0	15.999777	30
CTAGCGG	325	0.0	15.753626	29
GACGTGA	360	0.0	15.554759	7
TCTAGCG	320	0.0	15.4997835	28
CGTGAAA	400	0.0	15.199788	9
GTCCTAC	550	0.0	15.143956	1
GGACGTG	680	0.0	15.055254	6
ATTAGAC	75	6.2495156E-4	14.932014	3
TCGTTTA	420	0.0	14.8569355	30
ACGTATG	65	0.004162454	14.769025	32
TACCGTC	315	0.0	14.729406	7
CGAGCCG	435	0.0	14.712439	15
CGTTTAT	425	0.0	14.682149	31
TAGGACG	720	0.0	14.663734	4
TTAGGAC	405	0.0	14.615993	3
>>END_MODULE