FastQCFastQC Report
Thu 2 Feb 2017
SRR4062975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1344870
Sequences flagged as poor quality0
Sequence length38
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT47440.35274784923449853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT35850.26656851591603653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT20570.1529515863986854No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCG18490.1374854075115067No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTA16360.12164744547800159No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAAC16200.12045773940975708No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGC15570.11577327176604431No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGAC15310.11383999940514697No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA14810.11012216794188286No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG14790.10997345468335229No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.10893246187363835No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCG14610.10863503535657722No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA14170.1053633436689048No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATG13830.1028352182738852No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAAT13610.10119937243004899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTT703.086643E-822.85618832
GTATCGC400.00448013320.0028826
TCTAGCG3150.018.28494828
CTAGCGG3250.017.72233629
ATACCGT3650.017.5367746
CCGTGTA550.001363832217.4564119
ACCGTCG3600.017.335838
TATACTG1404.0017767E-1117.1453275
TACCGTC3750.017.0691287
AATCGCT1456.91216E-1116.55103115
CGTCGTA3750.016.21386110
TAGTACT1102.0183143E-716.0011164
TCGAACT908.586379E-615.99933219
CCGTCGT3850.015.7938979
TAAACGC3250.015.75318828
TAGCGGC3700.015.566915530
CAAATCG1753.6379788E-1215.54220713
GCATTAC1255.12955E-815.3622137
TATTAGC2300.015.3042762
GTCGTAG4000.015.20049511
Produced by FastQC (version 0.11.5)